US5087369A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fluidized bed separation and recovery of proteins from fluids in a rotary column
Assignee: Snow Brand Milk Products Co Ltd
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Filing: 1991-01-22
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Granted: 1992-02-11
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Expires: 2011-01-22
Abstract
Abstract Disclosed is a method for separating and recovering very small amounts of biologically active proteins (such as enzymes, antigens and antibodies) present in a various fluids, with a high recovery and on a large scale, in which a fluid containing a desired protein is introduced into a rotary column through orifices therein and brought into contact with a gel carrier capable of selectively adsorbing the desired protein, while the carrier is being gentle fluidized in a liquid by the rotary motion successively by wash water and eluting fluid, so that the column, after which the fluid is displaced, the carrier having the desired protein adsorbed thereon is washed and the desired protein is recovered therefrom by elution while the carrier fluidized.
Inventors
- 👤 Morimasa Tanimoto
- 👤 Kaoru Sato
- 👤 Shinichi Dosako
- 👤 Yoshihiko Honda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 04:19
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/14; C07K1/16; C07K1/22; C07K14/00; C07K14/195; C07K14/235; C07K14/435; C07K14/79
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
182 words
In a method for removing and recovering a specific protein or specific proteins from a protein-containing fluid by means of an affinity gel carrier having thereon a substance or group which imparts an affinity to the carrier for the protein, onto which the protein or proteins are absorbed, washed while absorbed thereon and eluted therefrom, the improvement which comprises the steps of charging a particulate affinity gel carrier having a particle size from 10 μm to 7 mm into a rotary column; passing the protein-containing fluid through the column by way of orifices while rotating the column to non-mechanically fluidize the carrier, whereby the fluid comes into contact with the carrier in a state of turbulent flow, until the protein contained therein becomes absorbed on the carrier; washing the column of fluidized carrier by passing a washing liquid through the fluidized carrier in the column being rotated; and eluting the desired protein from the washed column of fluid carrier by passing an eluent through the column being rotated without causing any significant damage to the carrier used to adsorb the protein thereon.
Claim 2
Dependent Claim
182 words
In a method for removing and recovering a specific protein or specific proteins from a protein-containing fluid by means of an affinity gel carrier having thereon a substance or group which imparts an affinity to the carrier for the protein, onto which the protein or proteins are absorbed, washed while absorbed thereon and eluted therefrom, the improvement which comprises the steps of charging a particulate affinity gel carrier having a particle size from 10 μm to 7 mm into a rotary column; passing the protein-containing fluid through the column by way of orifices while rotating the column to non-mechanically fluidize the carrier, whereby the fluid comes into contact with the carrier in a state of turbulent flow, until the protein contained therein becomes absorbed on the carrier; washing the column of fluidized carrier by passing a washing liquid through the fluidized carrier in the column being rotated; and eluting the desired protein from the washed column of fluid carrier by passing an eluent through the column being rotated without causing any significant damage to the carrier used to adsorb the protein thereon.
Claim 3
Dependent Claim
18 words
A method as claimed in claim 1 wherein the protein-containing fluid is lactoferrin-containing milk or lactoferrin-containing skim milk.
Claim 4
Dependent Claim
33 words
A method as claimed in claim 2 wherein the protein-containing fluid is lactoferrin-containing skim milk, the washing liquid is an aqueous sodium chloride solution, and the eluent is an aqueous sodium chloride solution.
Claim 5
Dependent Claim
17 words
The lactoferrin obtained by the method of claim 3 having a purity of about 80% or higher.
Claim 6
Dependent Claim
44 words
A method as claimed in claim 2 wherein the protein-containing fluid is lactoferrin-containing skim milk, the carrier is a carrier gel having monoclonal anti-lactoferrin antibody immobilized thereon, the washing liquid is an aqueous sodium chloride solution, and the eluent is an acetate buffer solution.
Claim 7
Dependent Claim
18 words
The lactoferrin obtained by the method of claim 5 and having a purity of about 90% or higher.
Claim 8
Dependent Claim
36 words
A method as claimed in claim 2 wherein the gel carrier is cellulose or chitosan having groups thereon which render the gel carrier effective for removing a specific protein or specific proteins from a protein-containing fluid.
Claim 9
Dependent Claim
13 words
A method as claimed in claim 7, wherein the groups are sulfate groups.
Claim 10
Dependent Claim
99 words
A method as claimed in claim 1 wherein the protein-containing fluid is passed through the column under the conditions: ##EQU3## where V 0 (liters) is the volume of the carrier container section of the column. (b) Rotational speed of column, r: 1rpm≦r≦1,000 rpm (c) Flow rate, V s (liters/hr): V.sub.s ≦10.sup.2 V.sub.0 (liters/hr) (d) Passage time, T (hr): ##EQU4## where C (g/liter) is the concentration in the fluid of the protein to be recovered, q is the saturated adsorption capacity (g/liter of gel) of the carrier, and V 1 is the volume of carrier (liters) charged into the column.
Claim 11
Dependent Claim
62 words
A method as claimed in claim 9, wherein the protein-containing fluid is lactoferrin-containing milk or lactoferrin-containing skim milk; wherein the gel carrier is cellulose or chitosan having groups thereon which render the gel carrier effective for removing the lactoferrin from the milk; and wherein the washing liquid is an aqueous sodium chloride solution and the eluent is an aqueous sodium chloride solution.
References Cited (Backward Citations)
US4072667A
Cited
US4156681A
Cited
US4116948A
Cited
US4111660A
Cited
US4190530A
Cited
US4242450A
Cited
JPS5643228A
Cited
US4422941A
Cited
US4663163A
Cited
US4675113A
Cited
US4668771A
Cited
US4667018A
Cited
US4741998A
Cited
US4791193A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-02-10 | 1995-08-12 | 1996-02-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-02-10 | 1999-08-12 | 2000-02-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-02-10 | 2003-08-12 | 2004-02-11 | $7700.00 | Window Closed / Lapsed |