US4741998A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Monoclonal antibody to MHS-5: a new probe for sexual assault analyses
Assignee: UVA Licensing and Ventures Group
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Filing: 1985-06-05
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Granted: 1988-05-03
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Expires: 2005-06-05
Abstract
Abstract A new probe for forensic analysis of sexual assaults is disclosed. The probe is a monoclonal antibody to a new protein marker, MHS-5, in semen. Also disclosed is the hybridoma producing the antibody as well as an assay utilizing the antibody for forensic analysis of criminal evidence.
Inventors
- 👤 John C. Herr
- 👤 Mark Sigman
- 👤 William M. Sutherland
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 04:28
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/8215; Y10S435/948; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
81 words
A probe for sexual assault analyses, comprising, an antibody which specifically binds to a seminal fluid antigen contained in male human ejaculate wherein said antibody has no cross reactivity with other human biological fluids, semen of an animal selected from a group consisting of rabbits, sheep, dogs, pigs, rhesus monkeys, cats, goats, squirrel monkeys, bulls, stallions, rats and mice, and human epididymal sperm, wherein said seminal fluid antigen has the identifying characteristics of the MHS-5 antigen and originates in seminal vesicles.
Claim 2
Dependent Claim
81 words
A probe for sexual assault analyses, comprising, an antibody which specifically binds to a seminal fluid antigen contained in male human ejaculate wherein said antibody has no cross reactivity with other human biological fluids, semen of an animal selected from a group consisting of rabbits, sheep, dogs, pigs, rhesus monkeys, cats, goats, squirrel monkeys, bulls, stallions, rats and mice, and human epididymal sperm, wherein said seminal fluid antigen has the identifying characteristics of the MHS-5 antigen and originates in seminal vesicles.
Claim 3
Dependent Claim
20 words
The probe of claim 1 wherein said antibody is the monoclonal antibody which is the product of hybridoma ATCC-HB 8830.
Claim 4
Dependent Claim
21 words
The probe of claim 2 wherein said monoclonal antibody binds to MHS-5 present in the semen of gorillas, chimpanzees and orangutans.
Claim 5
Dependent Claim
20 words
The ATCC-HB-8830 hybridoma cell line which produces a monoclonal antibody to a seminal fluid antigen contained in male human ejaculate.
Claim 6
Dependent Claim
20 words
The ATCC-HB-8830 hybridoma cell line which produces a monoclonal antibody to a seminal fluid antigen contained in male human ejaculate.
Claim 7
Independent Claim
49 words
A method for making probes for sexual assault analyses, comprising the steps of: (a) culturing hybridoma ATCC HB 8830 in an in vitro culture medium or in vivo under conditions suitable for the production by said hybridoma of monoclonal antibodies; and (b) harvesting monoclonal antibodies generated by said hybridoma.
Claim 8
Independent Claim
49 words
A method for making probes for sexual assault analyses, comprising the steps of: (a) culturing hybridoma ATCC HB 8830 in an in vitro culture medium or in vivo under conditions suitable for the production by said hybridoma of monoclonal antibodies; and (b) harvesting monoclonal antibodies generated by said hybridoma.
Claim 9
Dependent Claim
63 words
A kit for for the analysis of sexual assault by enzyme linked immunosorbent assay comprising an antibody which specifically binds to the MHS-5 human seminal fluid antigen in a biological fluid and reagent means for detecting the binding of said antibody to the MHS-5 antigen, wherein said antibody and said means are present in amounts sufficient to perform said enzyme linked immunosorbent assay.
Claim 10
Dependent Claim
63 words
A kit for for the analysis of sexual assault by enzyme linked immunosorbent assay comprising an antibody which specifically binds to the MHS-5 human seminal fluid antigen in a biological fluid and reagent means for detecting the binding of said antibody to the MHS-5 antigen, wherein said antibody and said means are present in amounts sufficient to perform said enzyme linked immunosorbent assay.
Claim 11
Dependent Claim
12 words
The kit of claim 6 wherein said antibody is an IgG antibody.
Claim 12
Dependent Claim
15 words
The kit of claim 7 wherein said IgG antibody is of the subclass IgGI antibody.
Claim 13
Dependent Claim
16 words
The kit of claim 6 wherein the antibody is the monoclonal antibody by hybridoma ATCC-HB 8830.
References Cited (Backward Citations)
US4511661A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-05-03 | 1991-11-02 | 1992-05-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-05-03 | 1995-11-02 | 1996-05-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-05-03 | 1999-11-02 | 2000-05-03 | $7700.00 | Window Closed / Lapsed |