US4422941A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Apparatus for liquid-solid column centrifugation chromatography and method
Assignee: University of Pittsburgh
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Filing: 1980-09-08
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Granted: 1983-12-27
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Expires: 2000-09-08
Abstract
Abstract Centrifugation chromatography columns including column members for containing a particle bed and a porous member underlying the bed. The column members have an upper receiving opening and a lower discharge opening. A receptacle is demountably secured to the column. Attachment is effected through cooperation between a lower portion of the column members and an upper portion of the receptacles. Support structures may be provided on the exterior of the column members to facilitate support thereof by a centrifuge. In another embodiment of the invention continuous column centrifugation chromatography is provided by means of a column rotor on which are mounted a plurality of columns. A collector rotor is provided with means for receiving material discharged from the columns. Relative synchronized movement between the column rotor and the collector rotor is provided. A distribution system for supplying liquid to be separated to the columns on a continuous basis is provided. Funnel means may be employed to facilitate transfer of effluent from the column to the collector rotor. A method for continuous column centrifugation chromatography employing a rotating array of columns which are subjected to a centrifugal force, effecting synchronized movement of an array of receptacles positioned to receive the liquid discharged from the columns and supplying on a continuous basis liquid to the columns.
Inventors
- 👤 Maurice H. Vaughan, Jr.
- 👤 Klaus B. Andersen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 21:01
CPC Classifications
G; G01; G01N; G01N30/00; G01N30/02; G01N30/26; G01N30/38; G01N30/46; G01N30/466; B; B01; B01D; B01D15/00; B01D15/08; B01D15/10; B01D15/18; B01J; B01J20/00; B01J20/281; B04; B04B; B04B5/00; B04B5/04; B04B5/0407; B04B5/0414; G01N30/60; G01N30/6004; G01N30/6017; G01N30/6047; B01J2220/00; B01J2220/50; B01J2220/54; G01N2030/381; G01N30/603
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
116 words
Column centrifugation chromatography apparatus comprising column rotor means for supporting and applying centrifugal force to a plurality of circumferentially spaced nonhelical centrifugation columns, said centrifugation columns being generally radially oriented, collector rotor means for collecting material discharged from said columns, a plurality of receptacles secured to said collector rotor means for receiving liquid discharged from said column, said receptacles disposed radially outwardly of said columns, power means for establishing synchronized relative movement of said column rotor means and said collector rotor means, said column rotor means and said collector rotor means being mounted for substantially coaxial rotation, and distribution means for supplying liquid to said columns while said column rotor means is subjected to said movement.
Claim 2
Dependent Claim
116 words
Column centrifugation chromatography apparatus comprising column rotor means for supporting and applying centrifugal force to a plurality of circumferentially spaced nonhelical centrifugation columns, said centrifugation columns being generally radially oriented, collector rotor means for collecting material discharged from said columns, a plurality of receptacles secured to said collector rotor means for receiving liquid discharged from said column, said receptacles disposed radially outwardly of said columns, power means for establishing synchronized relative movement of said column rotor means and said collector rotor means, said column rotor means and said collector rotor means being mounted for substantially coaxial rotation, and distribution means for supplying liquid to said columns while said column rotor means is subjected to said movement.
Claim 3
Dependent Claim
40 words
The column centrifugation chromatography apparatus of claim 1 wherein at least one of said column rotor means and said collector rotor means being secured to a drive shaft, and said power means including motor means for rotating said drive shaft.
Claim 4
Dependent Claim
31 words
The column centrifugation chromatography apparatus of claim 1 wherein said distribution means including reservoir means mounted for rotation with said column rotor means and means connecting said reservoir with said columns.
Claim 5
Dependent Claim
29 words
The column centrifugation chromatography apparatus of claim 3 wherein said reservoir means are supported by post means disposed generally on the axis of rotation of said column rotor means.
Claim 6
Dependent Claim
32 words
The column centrifugation chromatography apparatus of claim 3 wherein said reservoir means has an open upper portion for receipt of liquid, and supply conduit means for delivering said liquid to said reservoir.
Claim 7
Dependent Claim
27 words
The column centrifugation chromatography apparatus of claim 3 wherein said reservoir means being closed, and supply conduit means sealingly connected to said reservoir and delivering liquid thereto.
Claim 8
Dependent Claim
17 words
The column centrifugation chromatography apparatus of claim 3 wherein said reservoir means has a unitary storage chamber.
Claim 9
Dependent Claim
17 words
The column centrifugation chromatography apparatus of claim 3 wherein said reservoir means having a number of chambers.
Claim 10
Dependent Claim
17 words
The column centrifugation chromatography apparatus of claim 3 wherein said reservoir means has a generally annular chamber.
Claim 11
Dependent Claim
20 words
The column centrifugation chromatography apparatus of claim 8 wherein said reservoir means has a number of annular generally concentric chambers.
Claim 12
Dependent Claim
34 words
The column centrifugation chromatography apparatus of claim 1 wherein said distribution means include tube means for delivery of liquid to said columns, and stopper means in communication with said tube means and said columns.
Claim 13
Dependent Claim
47 words
The column centrifugation chromatography apparatus of claim 11 wherein said stopper means include a stopper having one passageway in communication with said tube means, and said passageway branching into a number of subpassageways for discharge of said liquid to several portions of the interior of said column.
Claim 14
Dependent Claim
20 words
The column centrifugation chromatography apparatus of claim 1 wherein said collector rotor means have an annular array of said receptacles.
Claim 15
Dependent Claim
33 words
The column centrifugation chromatography apparatus of claim 1 wherein said receptacles are fixedly mounted with their openings facing generally upwardly and radially inwardly with respect to the center of said collector rotor means.
Claim 16
Dependent Claim
27 words
The column centrifugation chromatography apparatus of claim 14 wherein said receptacles have mouths which diverge generally radially inwardly with respect to the longitudinal axis of said receptacles.
Claim 17
Dependent Claim
31 words
The column centrifugation chromatography apparatus of claim 1 wherein funnel means are interposed between said columns and said receptacles for facilitating efficient transfer of liquid from said columns to said receptacles.
Claim 18
Dependent Claim
31 words
The column centrifugation chromatography apparatus of claim 16 wherein said funnel means include an annular member having a plurality of generally radially oriented openings, and said openings diverge generally radially inwardly.
Claim 19
Dependent Claim
18 words
The column centrifugation chromatography apparatus of claim 1 wherein said receptacles are formed integrally within an annular member.
Claim 20
Dependent Claim
35 words
The column centrifugation chromatography apparatus of claim 1 wherein said receptacles are rotatably secured to said collector rotor means, whereby application of centrifugal force thereto will result in the receptacles rotating generally outwardly and upwardly.
Claim 21
Dependent Claim
30 words
The centrifugation chromatography apparatus of claim 1 including said column rotor means having means for supporting chromatography columns, and means for effecting rotation of said columns about their longitudinal axis.
Claim 22
Independent Claim
65 words
A method of continuous column centrifugation chromatography comprising providing an annular array of generally radially oriented columns, applying a centrifugal force to said array of columns through rotation of said array, effecting synchronized relative movement of an array of receptacles positioned generally radially outwardly of said columns to receive liquid discharge from said columns, and supplying on a continuous basis liquid to said centrifuge columns.
Claim 23
Independent Claim
65 words
A method of continuous column centrifugation chromatography comprising providing an annular array of generally radially oriented columns, applying a centrifugal force to said array of columns through rotation of said array, effecting synchronized relative movement of an array of receptacles positioned generally radially outwardly of said columns to receive liquid discharge from said columns, and supplying on a continuous basis liquid to said centrifuge columns.
Claim 24
Dependent Claim
26 words
The method of chromatography of claim 21 including providing chromatographic bed material in said columns, and removing effluent from said columns on a substantially continuous basis.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-12-26 | 1987-06-27 | 1987-12-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-12-26 | 1991-06-27 | 1991-12-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-12-26 | 1995-06-27 | 1995-12-27 | $7700.00 | Window Closed / Lapsed |