US3856669A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Elution centrifuge-apparatus and method
Assignee: DEPARTMENT OF HEALTH EDUCATION
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Filing: 1973-07-02
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Granted: 1974-12-24
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Expires: 1993-07-02
Abstract
Abstract An apparatus for elution centrifugation used in countercurrent chromatography comprises a high speed centrifuge head which revolves around a central vertical axis, a cylindrical column holder which is horizontally and rotatably carried by the centrifuge head, and a separation column fixedly disposed within the holder. The separation column includes a fine tube which passes externally introduced fluids to and from the centrifuge head and the cylindrical column holder without the use of rotating seals. The seals are eliminated by rotating the cylindrical column holder about its own axis while, simultaneously, revolving it around the central vertical axis of the apparatus at the same angular velocity.
Inventors
- 👤 Y Ito
- 👤 R Bowman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 09:54
CPC Classifications
G; G01; G01N; G01N30/00; G01N30/02; G01N30/26; G01N30/38; G01N30/42
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
116 words
AN ELUTION CENTRIFUGE COMPRISING: A SEPARATION COLUMN THE AXIS OF WHICH IS GENERALLY PERPENDICULAR TO A MAIN AXIS OF REVOLUTION; A FEED TUBE FOR INTRODUCING FLUIDS TO SAID SEPARATION COLUMN AND MEANS FOR PASSING FLUID TO SAID FEED TUBE; A RETURN TUBE FOR DISCHARGING FLUIDS FORM SAID SEPARATION COLUMN AND MEANS FOR RECEIVING FLUID THEREFROM; CENTRIFUGATION MEANS FOR REVOLVING SAID SEPARATION COLUMN ABOUT THE MAIN AXIS OF REVOLUTION AND FOR SIMULTANEOUSLY ROTATING SAID SEPARATION COLUMN ABOUT ITS AXIS AT THE SAME ANGULAR VELOCITY TO PREVENT TWISTING OF SAID FEED AND RETURN TUBES; AND A STATIONARY GUIDE MEANS, LOCATED OUTSIDE SAID CENTRIFUGATION MEANS COAXIAL WITH THE MAIN AXIS OF REVOLUTION, FOR GUIDING SAID FEED AND RETURN TUBES.
Claim 2
Dependent Claim
116 words
AN ELUTION CENTRIFUGE COMPRISING: A SEPARATION COLUMN THE AXIS OF WHICH IS GENERALLY PERPENDICULAR TO A MAIN AXIS OF REVOLUTION; A FEED TUBE FOR INTRODUCING FLUIDS TO SAID SEPARATION COLUMN AND MEANS FOR PASSING FLUID TO SAID FEED TUBE; A RETURN TUBE FOR DISCHARGING FLUIDS FORM SAID SEPARATION COLUMN AND MEANS FOR RECEIVING FLUID THEREFROM; CENTRIFUGATION MEANS FOR REVOLVING SAID SEPARATION COLUMN ABOUT THE MAIN AXIS OF REVOLUTION AND FOR SIMULTANEOUSLY ROTATING SAID SEPARATION COLUMN ABOUT ITS AXIS AT THE SAME ANGULAR VELOCITY TO PREVENT TWISTING OF SAID FEED AND RETURN TUBES; AND A STATIONARY GUIDE MEANS, LOCATED OUTSIDE SAID CENTRIFUGATION MEANS COAXIAL WITH THE MAIN AXIS OF REVOLUTION, FOR GUIDING SAID FEED AND RETURN TUBES.
Claim 3
Dependent Claim
66 words
The device of claim 1 wherein said centrifugation means includes: a drive shaft located on said axis of rotation; a motor for rotating said drive shaft; and a centrifuge head perpendicularly attached to said drive shaft, said centrifuge head comprising at one end a separation column holder rotatable about the axis of rotation of said separation column and an adjustable counterweight located at the opposite end.
Claim 4
Dependent Claim
81 words
The device of claim 2 wherein said centrifugation means for simultaneously rotating said separation column further includes: a first drive means coaxially located about said drive shaft; a second drive means engaging said rotatable separation column holder and disposed in a plane perpendicular to said first drive means; an endless belt connecting said first and second drive means; and means disposed on both sides of said first and second drive means for changing the direction of said endless belt by 90*.
Claim 5
Dependent Claim
17 words
The device of claim 3 wherein said first and second drive means are pulleys of equal diameter.
Claim 6
Dependent Claim
52 words
The device of claim 4 wherein said means for changing the direction of the endless belt are idler pulleys, said idler pulleys being rotatable about an axis defined by the intersection of the planes of rotation of said pulleys and spaced apart a distance approximately equal to the diameter of the pulleys.
Claim 7
Dependent Claim
48 words
The device of claim 1 wherein said stationary guide means comprises: a cylindrical hollow sleeve; said sleeve having first and second ends, the first end lying approximately on the axis of rotation of said separation column; and a polytetrafluoroethylene collar disposed about and extending past said first end.
Claim 8
Independent Claim
157 words
A method of countercurrent chromatography comprising: filling a separation column through a feed tube with a first solvent, said separation column having an axis of rotation perpendicular to a main axis of revolution, the feed tube being located coaxial with the main axis of rotation; centrifuging said filled separation column by revolving said filled separation column in a plane perpendicular to the main axis of revolution, the main axis of revolution lying outside said separation column, at various predetermined angular velocities and in a predetermined direction while simultaneously rotating said separation column about its own axis of rotation at the same angular velocity as the revolution of said separation column and in a direction whereby the feed tube will not twist; introducing a sample solute to be separated into the moving separation column; pumping a second solvent, immmiscible with said first solvent, into the moving separation column; and recovering the separating solute fractions leaving the separation column.
Claim 9
Independent Claim
157 words
A method of countercurrent chromatography comprising: filling a separation column through a feed tube with a first solvent, said separation column having an axis of rotation perpendicular to a main axis of revolution, the feed tube being located coaxial with the main axis of rotation; centrifuging said filled separation column by revolving said filled separation column in a plane perpendicular to the main axis of revolution, the main axis of revolution lying outside said separation column, at various predetermined angular velocities and in a predetermined direction while simultaneously rotating said separation column about its own axis of rotation at the same angular velocity as the revolution of said separation column and in a direction whereby the feed tube will not twist; introducing a sample solute to be separated into the moving separation column; pumping a second solvent, immmiscible with said first solvent, into the moving separation column; and recovering the separating solute fractions leaving the separation column.
Claim 10
Dependent Claim
15 words
The method of claim 7 wherein said first solvent is heavier than said second solvent.
Claim 11
Dependent Claim
15 words
The method of claim 7 wherein said first solvent is lighter than said second solvent.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-12-23 | 1978-06-24 | 1978-12-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-12-23 | 1982-06-24 | 1982-12-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-12-23 | 1986-06-24 | 1986-12-24 | $7700.00 | Window Closed / Lapsed |