Patent US3787870A successfully refreshed from Google Patents.
US4156681A
Expired / Lapsed
US
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Process for isolating albumin from blood
Assignee: Armour Pharmaceutical Co
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Filing: 1976-12-28
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Granted: 1979-05-29
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Expires: 1996-12-28
Abstract
Abstract Method for the abstraction of pure serum albumin from blood which includes the steps of separating the blood plasma from the solid constituents of the blood and isolating the dissolved non-albumin constituents from plasma, adding an albumin-stabilizer and treating such fluid with a lower aliphatic alcohol, whereby, the treatment is carried out at a volume concentration of alcohol of 7 to 14% in the presence of 0.001 to 0.1 moles of the stabilizer, at a temperature of from 60° to 75° C. and pH of from 4.5 to 7.5; the concomitant proteins, partly or largely denatured, are precipitated; the resultant solution containing pure serum albumin is separated from the precipitate at a temperature of from 1° to 30° C., preferably at room temperature.
Inventors
- 👤 Waldemar Schneider
- 👤 Christian Frohlich
- 👤 Harald Fiedler
- 👤 Hans Lefevre
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 00:36
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/76; C07K14/765; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83; Y10S530/831; Y10S530/85; Y10S530/851
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
140 words
A method for the abstraction of pure serum albumin from blood plasma, blood products, and other bodily fluids or tissue extracts for use in therapy which comprises the steps of: (a) separating the blood plasma from the solid constituents of the blood; (b) adding 0.001 to 0.1 moles of an albumin stabilizer; (c) treating with a lower aliphatic alcohol having the composition (CH 3 --(CH 2 ) n --OH, with n equal 0, 1 or 2 at a volume concentration of 7 to 4%; (d) said separating, adding, and treating steps being carried out at a temperature of from 60° to 75° C. and a pH from 4.5 to 7.5; (e) precipitating the concomitant proteins, partly or largely denatured; and (f) separating the resultant solution containing pure serum albumin from the precipitate at a temperature of 1° to 30° C.
Claim 2
Independent Claim
140 words
A method for the abstraction of pure serum albumin from blood plasma, blood products, and other bodily fluids or tissue extracts for use in therapy which comprises the steps of: (a) separating the blood plasma from the solid constituents of the blood; (b) adding 0.001 to 0.1 moles of an albumin stabilizer; (c) treating with a lower aliphatic alcohol having the composition (CH 3 --(CH 2 ) n --OH, with n equal 0, 1 or 2 at a volume concentration of 7 to 4%; (d) said separating, adding, and treating steps being carried out at a temperature of from 60° to 75° C. and a pH from 4.5 to 7.5; (e) precipitating the concomitant proteins, partly or largely denatured; and (f) separating the resultant solution containing pure serum albumin from the precipitate at a temperature of 1° to 30° C.
Claim 3
Dependent Claim
13 words
A method according to claim 1, wherein the lower aliphatic alcohol is ethanol.
Claim 4
Dependent Claim
17 words
A method according to claim 2, wherein the content of alcohol is 8 to 12% by volume.
Claim 5
Dependent Claim
19 words
A method according to claim 1, wherein the albumin-stabiliser is sodium caprylate in a 0.003 to 0.005 mole concentration.
Claim 6
Dependent Claim
16 words
A method according to claim 1, wherein the pH-value of the treatment is 6 to 7.
Claim 7
Dependent Claim
14 words
A method according to claim 1, wherein the temperature is 67° to 69° C.
Claim 8
Dependent Claim
48 words
A method according to claim 1, wherein the solution containing pure albumin is separated from the precipitate by alluvial filtration through a fabric filter element, thereby collecting the non-albumin constituents together with a filtering aid on the filter elements and gaining a clear albumin-solution as a filtered product.
Claim 9
Dependent Claim
34 words
The process according to claim 7, characterized by performing said filtration within a centrifugal purification filter comprising a sealed pressure vessel, said filter elements having a mesh size of between 20 and 200 microns.
Claim 10
Dependent Claim
23 words
The process according to claim 8, characterized in that one of said filter elements has a mesh size from 70 to 90 microns.
Claim 11
Dependent Claim
30 words
The process according to claim 8, characterized by performing said filtration with horizontally disposed filter elements comprising metallic twisted (wire) or braided fabrics having a mesh size of 80 microns.
Claim 12
Dependent Claim
24 words
The process according to claim 7, characterized by mixing a suspension of from about 30 to 70 grams of kieselguhr filtering aid per liter.
Claim 13
Dependent Claim
27 words
The process according to claim 7, characterized by, that the clear albumin-solution is purified by diafiltration (filtration of water and known albumin constituents through a dialysis membrane).
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-05-28 | 1982-11-27 | 1983-05-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-05-28 | 1986-11-27 | 1987-05-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-05-28 | 1990-11-27 | 1991-05-29 | $7700.00 | Window Closed / Lapsed |