Plasma fractionation process

Claim 1

A PROCESS FOR THE FRACTIONATION OF HUMAN BLOOD PLASMA TO OBTAIN A FRACTION HAVING THE ABILITY TO ENHANCE THE AVIDITY OF RH TYPING SERA, COMPRISING THE STEPS OF: ADDING TO HUMAN BLOOD PLASMA AT LEAST FOUR VOLUMES, BASED ON THE VOLUME OF PLASMA, OF AFIRST SOLUTION, ADJUSTED TO A PH OF 4 TO 5, AND CONTAINING 20% TO 30% BY WEIGHT OF A LOW MOLECULAR WEIGHT WATER-SOLUBLE ALIPHATIC ALCOHOL, 40 TO 90 MILLIMOLS OF A LOW MOLECULAR WEIGHT WATER AND ALCOHOL SOLUBLE ORGANIC ACID HAVING AT LEAST TWO CARBON ATOMS AND HAVING THE ABILITY TO ACT AS A BUFFER IN THE PH RANGE OF 4 TO 5, STIRRING AND MAINTAINING THE TERMPERATURE OF THE MIXTURE OF THE SOLUTION AND PLASMA AT A TEMPERATURE OF 0* C. 5* C. UNTIL A PRECIPITATE IS FORMED, REMOVING THE PRECIPITATE FROM THE MIXTURE AT A TEMPERATURE OF 0* C. TO 5* C.; EXTRACTING THE PRECIPITATE WITH AT LEAST TWO VOLUMES, BASED ON THE ORIGINAL VOLUME OF THE PLASMA, OF A SECOND SOLUTION, ADJUSTED TO A PH OF 4.8 TO 5.5, AND CONTAINING 5% TO 8% BY VOLUME OF A LOW MOLECULAR WEIGHT WATER-SOLUBLE ALIPHATIC ALCOHOL, 0.4 TO 0.5 MOL PER LITER OF A LOW MOLECULAR WEIGHT WATER-SOLUBLE ALPHA AMINO ACID, AND 35 TO 65 MILLIMOLS OF A LOW MOLECULAR WEIGHT WATER-SOLUBLE WEAK ORGANIC ACID HAVING AT LEAST TWO CARBON ATOMS AND HAVING THE ABILITY TO ACT AS A BUFFER IN A PH RANGE OF 4.8 TO 5.5, STIRRING THE MIXTURE OF THE SECOND SOLUTION AND PRECIPITATE AND SEPARATING THE INSOLUBLE PORTION OF THE PRECIPITATE; EXTRACTING THE INSOLUBLE PORTION OF THE PRECIPITATE WITH AT LEAST FOUR VOLUMES, BASED ON THE ORIGINAL VOLUME OF THE PLASMA, OF A THIRD SOLUTION, ADJUSTED TO A PH OF 6.6 TO 7.0, AND CONTAINING 5% TO 8% BY VOLUME OF A LOW MOLECULAR WEIGHT WATER-SOLUBLE ALIPHATIC ALCOHOL, 0.4 TO 0.5 MOL PER LITER OF A LOW MOLECULAR WEIGHT WATERSOLUBLE ALPHA AMINO ACID, AND AN ACID HAVING THE ABILITY TO ACT AS A BUFFER IN A PH RANGE OF 6.6 TO 7.0, STIRRING THE MIXTURE AND SEPARATING THE INSOLUBLE PORTION.