Patent US5079351A successfully refreshed from Google Patents.
US5079351A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Oligonucleotides and kits for detection of htlvi and htlvii viruses by hybridization
Assignee: Roche Molecular Systems Inc
•
Filing: 1986-11-26
•
Granted: 1992-01-07
•
Expires: 2006-11-26
Abstract
Abstract The presence or absence of a nucleic acid sequence of an isolate of HTLVI and/or HTLVII in a sample containing one or more nucleic acids and suspected of containing such sequence can be detected by amplifying the sequence using primers to form extension products as templates and detecting the amplified product if it is present. This may be accomplished by adding a labeled hydridization probe to the amplified product, either free in solution or after immobilization on a solid support.
Inventors
- 👤 John J. Sninsky
- 👤 Shirley Y. Kwok
- 👤 Bernard Poiesz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 18:10
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/70; C12Q1/701; C12Q1/702; C12Q1/703; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
77 words
A kit for detecting or monitoring a nucleic acid sequence which is substantially conserved among the isolates of both HTLVI and HTLVII virus nucleic acids which nucleic acid sequence is specific to the nucleic acids in both HTLVI and HTLVII isolates and which nucleic acid sequence is suspected of being contained in a sample, which kit comprises: (a) two oligonucleotide primers, wherein the primers are: 5'-CGGATACCCAGTCTACGTGT-3' and 5'-GAGCCGATAACGCGTCCATCG-3', and (b) a probe, wherein the probe is 5'-ACGCCCTACTGGCCACCTGTCCAGAGCATCAGATCACCTG-3'.
Claim 2
Dependent Claim
77 words
A kit for detecting or monitoring a nucleic acid sequence which is substantially conserved among the isolates of both HTLVI and HTLVII virus nucleic acids which nucleic acid sequence is specific to the nucleic acids in both HTLVI and HTLVII isolates and which nucleic acid sequence is suspected of being contained in a sample, which kit comprises: (a) two oligonucleotide primers, wherein the primers are: 5'-CGGATACCCAGTCTACGTGT-3' and 5'-GAGCCGATAACGCGTCCATCG-3', and (b) a probe, wherein the probe is 5'-ACGCCCTACTGGCCACCTGTCCAGAGCATCAGATCACCTG-3'.
Claim 3
Dependent Claim
62 words
A kit for detecting or monitoring an HTLVI virus nucleic acid sequence, which HTLVI nucleic acid sequence is specific to the nucleic acids in HTLVI isolates, and which nucleic acid sequence is suspected of being contained in a sample, which kit comprises: (a) two oligonucleotide primers, wherein the primers are: 5'-CTTCACAGTCTCTACTGTGC-3' and 5'-CGGCAGTTCTGTGACAGGG-3'; and (b) a probe, wherein the probe is 5'-CCGCAGCTGCACTAATGATTGAACTTGAGAAGGAT-3'.
Claim 4
Dependent Claim
62 words
A kit for detecting or monitoring an HTLVI virus nucleic acid sequence, which HTLVI nucleic acid sequence is specific to the nucleic acids in HTLVI isolates, and which nucleic acid sequence is suspected of being contained in a sample, which kit comprises: (a) two oligonucleotide primers, wherein the primers are: 5'-CTTCACAGTCTCTACTGTGC-3' and 5'-CGGCAGTTCTGTGACAGGG-3'; and (b) a probe, wherein the probe is 5'-CCGCAGCTGCACTAATGATTGAACTTGAGAAGGAT-3'.
Claim 5
Dependent Claim
62 words
A kit for detecting or monitoring an HTLVII virus nucleic acid sequence, which HTLVII nucleic acid sequence is specific to the nucleic acids in HTLVII isolates, and which nucleic acid sequence is suspected of being contained in a sample, which kit comprises: (a) two oligonucleotide primers, wherein the primers are: 5'-ATCTACCTCCACCATGTCCG-3' and 5'-TCAGGGGAACAAGGGGAGCT-3'; and (b) a probe, wherein the probe is 5'-TAAGGGAGTCTGTGTATTCATTGAAGGTGGAAATTGGGTC-3'.
Claim 6
Dependent Claim
62 words
A kit for detecting or monitoring an HTLVII virus nucleic acid sequence, which HTLVII nucleic acid sequence is specific to the nucleic acids in HTLVII isolates, and which nucleic acid sequence is suspected of being contained in a sample, which kit comprises: (a) two oligonucleotide primers, wherein the primers are: 5'-ATCTACCTCCACCATGTCCG-3' and 5'-TCAGGGGAACAAGGGGAGCT-3'; and (b) a probe, wherein the probe is 5'-TAAGGGAGTCTGTGTATTCATTGAAGGTGGAAATTGGGTC-3'.
Claim 7
Dependent Claim
40 words
An oligonucleotide primer complementary to HTLVI and/or HTLVII nucleic acids, that comprises a nucleic acid sequence between 15 and 21 nucleotides in length selected from the group of nucleic acid sequences consisting of: 5'-CGGATACCCAGTCTACGTGT; 5'-GAGCCGATAACGCGTCCATCG; 5'-CTTCACAGTCTCTACTGTGC; 5'-CGGCAGTTCTGTGACAGGG; 5'-ATCTACCTCCACCATGTCCG; and 5'-TCAGGGGAACAAGGGGAGCT.
Claim 8
Dependent Claim
40 words
An oligonucleotide primer complementary to HTLVI and/or HTLVII nucleic acids, that comprises a nucleic acid sequence between 15 and 21 nucleotides in length selected from the group of nucleic acid sequences consisting of: 5'-CGGATACCCAGTCTACGTGT; 5'-GAGCCGATAACGCGTCCATCG; 5'-CTTCACAGTCTCTACTGTGC; 5'-CGGCAGTTCTGTGACAGGG; 5'-ATCTACCTCCACCATGTCCG; and 5'-TCAGGGGAACAAGGGGAGCT.
Claim 9
Dependent Claim
20 words
The primer of claim 4 that is complementary to HTLVI and HTLVII nucleic acids and comprises the nucleotide sequence 5'-CGGATACCCAGTCTACGTGT-3'.
Claim 10
Dependent Claim
20 words
The primer of claim 4 that is complementary to HTLVI and HTLVII nucleic acids and comprises the nucleotide sequence 5'-GAGCCGATAACGCGTCCATCG-3'.
Claim 11
Dependent Claim
18 words
The primer of claim 4 that is complementary to HTLVI nucleic acids and comprises the nucleotide sequence 5'-CTTCACAGTCTCTACTGTGC-3'.
Claim 12
Dependent Claim
18 words
The primer of claim 4 that is complementary to HTLVI nucleic acids and comprises the nucleotide sequence 5'-CGGCAGTTCTGTGACAGGG-3'.
Claim 13
Dependent Claim
18 words
The primer of claim 4 that is complementary to HTLVI nucleic acids and comprises the nucleotide sequence 5'-ATCTACCTCCACCATGTCCG-3'.
Claim 14
Dependent Claim
18 words
The primer of claim 4 that is complementary to HTLVI nucleic acids and comprises the nucleotide sequence 5'-TCAGGGGAACAAGGGGAGCT-3'.
Claim 15
Dependent Claim
46 words
A DNA probe capable of hybridizing with an HTLVI and/or an HTLVII nucleic acid, that comprises a nucleic acid sequence between 15 and 40 nucleotides in length selected from the group of nucleic acid sequences, or a sequence complementary thereto, consisting of: 5'-ACGCCCTACTGGCCACCTGTCCAGAGCATCAGATCACCTG; 5'-CCGCAGCTGCACTAATGATTGAACTTGAGAAGGAT; and 5'-TAAGGGAGTCTGTGTATTCATTGAAGGTGGAAATTGGGTC.
Claim 16
Dependent Claim
46 words
A DNA probe capable of hybridizing with an HTLVI and/or an HTLVII nucleic acid, that comprises a nucleic acid sequence between 15 and 40 nucleotides in length selected from the group of nucleic acid sequences, or a sequence complementary thereto, consisting of: 5'-ACGCCCTACTGGCCACCTGTCCAGAGCATCAGATCACCTG; 5'-CCGCAGCTGCACTAATGATTGAACTTGAGAAGGAT; and 5'-TAAGGGAGTCTGTGTATTCATTGAAGGTGGAAATTGGGTC.
Claim 17
Dependent Claim
30 words
The probe of claim 11 that is capable of hybridizing to HTLVI and HTLVII nucleic acids in a sample and comprises the nucleotide sequence or a sequence complementary to 5'-ACGCCCTACTGGCCACCTGTCCAGAGCATCAGATCACCTG-3'.
Claim 18
Dependent Claim
28 words
The probe of claim 11 that is capable of hybridizing to HTLVI nucleic acids in a sample and comprises the nucleotide sequence or a sequence complementary to 5'-CCGCAGCTGCACTAATGATTGAACTTGAGAAGGAT-3'.
Claim 19
Dependent Claim
28 words
The probe of claim 11 that is capable of hybridizing to HTLVI nucleic acids in a sample and comprises the nucleotide sequence or a sequence complementary to 5'-TAAGGGAGTCTGTGTATTCATTGAAGGTGGAAATTGGGTC-3'.
References Cited (Backward Citations)
EP0062286A1
Cited
EP0120658A2
Cited
US4520113A
Cited
EP0173529A1
Cited
EP0173339A2
Cited
EP0178978A1
Cited
EP0185444A2
Cited
EP0187041B1
Cited
US4683202A
Cited
US4683202B1
Cited
US4683195A
Cited
US4683195B1
Cited
US4806463A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-01-06 | 1995-07-08 | 1996-01-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-01-06 | 1999-07-08 | 2000-01-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-01-06 | 2003-07-08 | 2004-01-07 | $7700.00 | Window Closed / Lapsed |