EP0173529A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Molecular clones of the genome of HTLV-III
Assignee: United States Department of Commerce
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Filing: 1985-08-19
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Granted: —
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Expires: 2005-08-19
Abstract
Abstract Disclosed is the molecular cloning of HTLV-III, the adult leukemia and acquired immune deficiency syndrome (AIDS) virus. Clone BH10 contains a 9.0 Kb viral insert constituting the entire HTLV-III genose. Clones BH8 and BH5 contain viral inserts of 5.5 Kb and 3.5 Kb, respectively. These clones are suitable for the development of diagnostic and therapeutic measures for AIDS, as well as use as probes for the detection of AIDS.
Inventors
- 👤 Flossie Wong-Staal
- 👤 Robert C. Gallo
- 👤 Beatrice H. Hahn
- 👤 Mikulas Popovic
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 08:18
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12Q; C12Q1/00; C12Q1/70; C12Q1/701; C12Q1/702; C12Q1/703; A; A61; A61K; A61K38/00; C12N; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/14011; C12N2740/14022
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
10 words
Recombinant clone BH10 characterized by containing the complete HTLV-III genome.
Claim 2
Dependent Claim
10 words
Recombinant clone BH10 characterized by containing the complete HTLV-III genome.
Claim 3
Dependent Claim
14 words
Recombinant clone BH8 characterized by containing a 5.5 Kb viral insert from HTLV-III virus.
Claim 4
Dependent Claim
14 words
Recombinant clone BH8 characterized by containing a 5.5 Kb viral insert from HTLV-III virus.
Claim 5
Dependent Claim
13 words
Recombinant clone BH5 characterized by containing a 3.5 Kb viral insert from HTLV-III.
Claim 6
Dependent Claim
13 words
Recombinant clone BH5 characterized by containing a 3.5 Kb viral insert from HTLV-III.
Claim 7
Dependent Claim
43 words
A process for the production of recombinant molecular clones of HTLV-III consisting essentially of cleaving unintegrated viral DNA from HTLV-III cells with a restriction enzyme to obtain a provirus, hybridizing radiolabeled cDNA to said provirus, and digesting said virus in a suitable plasmid.
Claim 8
Dependent Claim
43 words
A process for the production of recombinant molecular clones of HTLV-III consisting essentially of cleaving unintegrated viral DNA from HTLV-III cells with a restriction enzyme to obtain a provirus, hybridizing radiolabeled cDNA to said provirus, and digesting said virus in a suitable plasmid.
Claim 9
Dependent Claim
81 words
A process for the molecular cloning and expression of cDNA sequence of HTLV-III consisting essentially of isolating total cellular mRNA from H9/HTLV-III cells; forming double-stranded cDNA from said mRNA and inserting said double-stranded cDNA into a phage lambda to form a recombinant DNA molecule; hybridizing said recombinant DNA molecule with a radiolabelled probe; removing cDNA from said molecules and inserting said cDNA into a suitable plasmid; and transfecting said plasmids into a suitable host cell capable of expressing HTLV-III DNA sequences.
Claim 10
Dependent Claim
81 words
A process for the molecular cloning and expression of cDNA sequence of HTLV-III consisting essentially of isolating total cellular mRNA from H9/HTLV-III cells; forming double-stranded cDNA from said mRNA and inserting said double-stranded cDNA into a phage lambda to form a recombinant DNA molecule; hybridizing said recombinant DNA molecule with a radiolabelled probe; removing cDNA from said molecules and inserting said cDNA into a suitable plasmid; and transfecting said plasmids into a suitable host cell capable of expressing HTLV-III DNA sequences.
Claim 11
Dependent Claim
11 words
A process of Claim 5 wherein said plasmid is λ BH10.
Claim 12
Dependent Claim
12 words
A process of Claim 5 wherein said plasmid is λ BH8 .
Claim 13
Dependent Claim
11 words
A process of Claim 5 wherein said plasmid is λ BH5.
Claim 14
Dependent Claim
14 words
A process of Claim 5 wherein said cDNA sequence corresponds to 9.0 Kb sequence.
Claim 15
Dependent Claim
15 words
A process of Claim 5 wherein said cDNA sequence corresponds to a 5.5 Kb sequence.
Claim 16
Dependent Claim
15 words
A process of Claim 5 wherein said cDNA sequence coresponds to a 3.5 Kb sequence.
Claim 17
Dependent Claim
25 words
A recombinant clone of HTLV-III selected from the group held in deposits in the American Type Culture Collection under accession numbers 40125; 40126 and 40127.
Claim 18
Dependent Claim
25 words
A recombinant clone of HTLV-III selected from the group held in deposits in the American Type Culture Collection under accession numbers 40125; 40126 and 40127.
Claim 19
Dependent Claim
34 words
A pharmaceutical preparation characterised by including a recombinant clone as claimed in any of Claims 1, 2, 3 and 12, or as produced by a process claimed in any of Claims 4 - 11.
Claim 20
Dependent Claim
51 words
The use of a recombinant clone as claimed in any of Claims 1, 2, 3 and 12, or as produced by a process claimed in any of Claims 4 - 11, in the preparation of a medicament for the treatment, or diagnosis of acquired immune deficiency syndrome (AIDS) or adult leukemia.
References Cited (Backward Citations)
EP0113078A2
Cited
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