EP0178978A1
Expired / Lapsed
EP
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Cloned DNA sequences, hybridizable with genomic RNA of lymphadenopathy-associated virus (LAV)
Assignee: Centre National de la Recherche Scientifique
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Filing: 1985-09-17
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Granted: —
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Expires: 2005-09-17
Abstract
Abstract The invention relates to cloned DNAs which contains a DNA fragment hybridizable with the genomic RNA of LAV viruses. They are useful as probes for the in vitro detection of viral infection in biological samples taken from persons possibly afflicted with AIDS.
Inventors
- 👤 Marc Alizon
- 👤 Françoise Barre Sinoussi
- 👤 Pierre Sonigo
- 👤 Pierre Tiollais
- 👤 Jean-Claude Chermann
- 👤 Luc Montagnier
- 👤 Simon Wain-Hobson
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 19:34
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; A; A61; A61K; A61K38/00; A61K40/00; C07K16/00; C07K16/08; C07K16/10; C07K16/112; C07K16/114; C07K16/1143; C12; C12Q; C12Q1/00; C12Q1/70; C12Q1/701; C12Q1/702; C12Q1/703; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; A61K39/00; A61K2039/505; C12N; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/16011; C12N2740/16022; C12N2740/16111; C12N2740/16122; C12N2740/16211; C12N2740/16222; C12N2740/16311; C12N2740/16322
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
25 words
A cloned DNA which contains a DNA which is hybridizable with the genomic RNA of the LAV viruses or a fragment of said hybridizable DNA.
Claim 2
Dependent Claim
25 words
A cloned DNA which contains a DNA which is hybridizable with the genomic RNA of the LAV viruses or a fragment of said hybridizable DNA.
Claim 3
Dependent Claim
25 words
The DNA of claim 1 which is a recombinant of said hybridizable DNA or DNA fragment hybridizable with the genomic RNA of the LAV virus.
Claim 4
Dependent Claim
17 words
The DNA of claim 1 or 2 wherein said hybridizable DNA or DNA fragment is a cDNA.
Claim 5
Dependent Claim
34 words
The DNA of claims 1 to 3 which contains the following restriction sites in the following order (from the 3' end to the 5' end) : Hind III, Sac I, Bgl II (LAV 75).
Claim 6
Dependent Claim
28 words
The DNA of claim 4 which contains the following restriction sites in the following order : Hind III, Sac I, Bgl II, Bgl II, Kpn I (LAV 82).
Claim 7
Dependent Claim
36 words
The DNA of claim 4 which contains the following restriction sites in the following order : Hind III, Sac I, Bgl II, Bgl II, Kpn I, XHo I, Bam HI, Hind III, Bgl II (LAV 13).
Claim 8
Dependent Claim
13 words
The DNA of claim 6 which has a size of about 2.5 kb.
Claim 9
Dependent Claim
38 words
The DNA of any of claims 1 to 7 which contains a region corresponding to the R and U3 regions of the LTR as well as to the 3' end of the coding region of the retroviral DNA.
Claim 10
Dependent Claim
15 words
The DNA of claim 1 which has a size from about 9.1 to 9.2 kb.
Claim 11
Dependent Claim
14 words
The DNA of claim 9 which contains the following series of restriction sites :
Claim 12
Dependent Claim
17 words
The DNA of claim 10 which contains an additional Hind III approximately at the 5 550 coordinate.
Claim 13
Dependent Claim
29 words
A DNA fragment according to claim 1 which comprises a sequence extending from approximately Kpn I (6100) to approximately Bam HI (8150) of the sequence defined in claim 11.
Claim 14
Dependent Claim
29 words
A DNA fragment according to claim 1 which comprises a sequence extending from approximately Kpn I (3500) to approximately Bgl II (6500) of the sequence defined in claim 11.
Claim 15
Dependent Claim
28 words
A DNA fragment according to claim 1 which comprises a sequence extending from approximately Pst (800) to approximately Kpn I (3500) of the sequence defined in claim 11.
Claim 16
Dependent Claim
12 words
A DNA fragment of claim 1 which codes for the enveloppe proteins.
Claim 17
Dependent Claim
12 words
A DNA fragment of claim 1 which codes for the retroviral polymerase.
Claim 18
Dependent Claim
9 words
A DNA fragment which codes for the core proteins.
Claim 19
Dependent Claim
9 words
A DNA fragment which codes for the core proteins.
Claim 20
Dependent Claim
22 words
A probe for the in vitro detection of LAV which consists of a DNA according to any of claims 1 to 17.
Claim 21
Dependent Claim
54 words
A method for the in vitro detection of viral infection due to the LAV viruses which comprises contacting a biological sample originating from a person to be diagnosed for LAV infection and containing DNA in a form suitable for hybridization with the probe of claim 18 under hybridizing conditions and detecting the hybridized probe.
Claim 22
Dependent Claim
40 words
An expression vector, particularly a plasmid, for the transformation of procaryotic or eucaryotic cells which contains an insert consisting of a DNA fragment hybridizable with the retroviral genome of LAV viruses as defined in any of claims 1 to 17.
Claim 23
Dependent Claim
13 words
The vector of claim 20 which contains the DNA fragment of claim 15.
Claim 24
Dependent Claim
29 words
A microorganism, eucaryotic or procaryotic cell which is transformed by a vector according to claim 19 or 20 and which expresses the polypeptide encoded by the corresponding DNA fragment.
Claim 25
Dependent Claim
6 words
The expression products of said microorganisms.
Claim 26
Dependent Claim
6 words
The expression products of said microorganisms.
Claim 27
Dependent Claim
14 words
The purified RNAs of LAV viruses which have sizes from 9.1 to 9.2 kb.
Claim 28
Dependent Claim
14 words
The purified RNAs of LAV viruses which have sizes from 9.1 to 9.2 kb.
References Cited (Backward Citations)
No citation records indexed.
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