Dry multilayer analytical element for analysis of enzymes or triglycerides

Claim 1

A dry multilayer analytical element for analysis of an enzyme or triglycerides in a fluid sample comprising: a support which is impervious to liquids and is light transmissive, a first layer on said support, said first layer comprising a dye forming precursor which is a tetrazolium salt selected from the group consisting of 3,3'-(3,3'-dimethoxy-4,4'-biphenylene)-bis [2-(p-nitrophenyl)-5-phenyltetrazolium chloride] (NBT), 3,3'-(3,3'-dimethoxy-4, 4'-biphenylene)-bis[2,5-diphenyltetrazolium chloride] (BT), 3-(4',5'-dimethyltriazolyl-2)-2,4-diphenyltetrazolium bromide (MTT), 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl-tetrazolium chloride (INT), 2,2',5,5'-tetra-(p-nitrophenyl)-3,3'-(3-dimethoxy-4-diphenylene)-ditetrazolium chloride (TNBT), 2,3,5-triphenyltetrazolium chloride (TT) and 3,3'-(4,4'-biphenylene)-bis [2,5-diphenyltetrazolium chloride] (NT), and a second layer provided adjacent to said first layer, said second layer comprising an electron-transmitting agent selected from the group consisting of N-methylphenazine methosulfates, meldra Blue, Methylene Blue and diaphorase, wherein: one of said first layer and said second layer comprises an oxidation co-enzyme, and one of said first layer and said second layer comprises a reagent comprising an enzyme substrate, enzyme or coenzyme other than an oxidation coenzyme, and said oxidation co-enzyme is oxidized nicotinamide adenine dinucleotide (NAD + ) or oxidized nicotinamide adenine dinucleotide phosphoric acid (NADP + ); and said reagent is operable to react with said oxidation co-enzyme to form a reduction co-enzyme, in the presence of said enzyme or said triglycerides in said fluid sample, whereby said reduction co-enzyme reduces said electron-transmitting agent to produce a reduced electron-transmitting agent and said reduced electron-transmitting agent reduces said tetrazolium salt to form a dye which can be detected and measured.