US4503145A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Quantitative analysis film
Assignee: Fujifilm Holdings Corp
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Filing: 1982-02-26
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Granted: 1985-03-05
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Expires: 2002-02-26
Abstract
Abstract An analysis film for assaying transaminase activity comprises a reagent layer containing pyruvic acid oxidase, a combination of a hydrogen donor and a coupler--which is capable of forming a cationic dye--and an anionic polymer. The cationic dye formed by the reaction with a color indicator composition for detecting hydrogen peroxide is fixed with the anionic polymer, thereby mobilization and diffusion of the cationic dye being prevented so that the analysis film achieves high detection sensitivity and high accuracy.
Inventors
- 👤 Harumi Katsuyama
- 👤 Yoshikazu Amano
- 👤 Asaji Kondo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 20:43
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/48; C12Q1/52; C12Q1/26; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
46 words
An analysis film comprising a reagent layer containing a transaminase substrate composition, a pyruvic acid oxidase composition, a cationic dye-forming color indicator for detecting hydrogen peroxide, and an anionic polymer containing a sulfonate group capable of reacting with formed cationic dye to fix the formed dye.
Claim 2
Dependent Claim
46 words
An analysis film comprising a reagent layer containing a transaminase substrate composition, a pyruvic acid oxidase composition, a cationic dye-forming color indicator for detecting hydrogen peroxide, and an anionic polymer containing a sulfonate group capable of reacting with formed cationic dye to fix the formed dye.
Claim 3
Dependent Claim
32 words
The analysis film of claim 1 wherein said pyruvic acid oxidase composition includes pyruvic acid oxidase, a phosphate source compound, flavine adenine dinucleotide, thiamine pyrophosphate, and a divalent or trivalent metal ion.
Claim 4
Dependent Claim
27 words
The analysis film of claim 2 wherein said color indicator composition for detecting hydrogen peroxide comprises a substance having peroxidase activity, a hydrogen donor and a coupler.
Claim 5
Dependent Claim
20 words
The analysis film of any one of claims 1, 2 or 3 wherein said reagent layer is a single layer.
Claim 6
Dependent Claim
63 words
The analysis film of any one of claims 1, 2 or 3 wherein said reagent layer is composed of at least two layers one of which is a transaminase substrate layer containing a transaminase substrate and one of which is a pyruvic acid oxidase color forming reaction dye fixing layer comprising a pyruvic acid oxidase composition, a color indicator and an anionic polymer.
Claim 7
Dependent Claim
61 words
The analysis film of any one of claims 1, 2 or 3 wherein said reagent layer comprises at least two layers one of which is a dye fixing layer containing an anionic polymer and one of which is a transaminase substrate-pyruvic acid oxidase color forming reaction layer comprising a transaminase substrate composition, a pyruvic acid oxidase composition and a color indicator.
Claim 8
Dependent Claim
50 words
The analysis film of any one of claims 1, 2 or 3 wherein said reagent layer possesses a support on one surface thereof and a porous layer on the other surface thereof and said porous layer is adhered to said reagent layer in a fluid contact as an integral form.
References Cited (Backward Citations)
US3867258A
Cited
JPS50111799A
Cited
US4042335A
Cited
US4166093A
Cited
US4153668A
Cited
US4246342A
Cited
US4260679A
Cited
JPS56122947A
Cited
US4316954A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-03-04 | 1988-09-03 | 1989-03-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-03-04 | 1992-09-03 | 1993-03-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-03-04 | 1996-09-03 | 1997-03-05 | $7700.00 | Window Closed / Lapsed |