US5041236A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Antimicrobial methods and compositions employing certain lysozymes and endoglycosidases
Assignee: Procter and Gamble Co
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Filing: 1989-10-27
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Granted: 1991-08-20
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Expires: 2009-10-27
Abstract
Abstract An antimicrobial composition comprising endo-β-N-acetylglucosaminidase and/or endoglycopeptidase, and ruminant stomach lysozyme is presented. A method for destroying or removing microbes by treatment with these enzymes is also presented.
Inventors
- 👤 Richard S. Carpenter
- 👤 Ann M. Wolff
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 07:32
CPC Classifications
C; C11; C11D; C11D3/00; C11D3/16; C11D3/38; C11D3/386; C11D3/38645; A; A01; A01N; A01N63/00; A01N63/50; A61; A61K; A61K8/00; A61K8/18; A61K8/30; A61K8/64; A61K8/66; A61P; A61P17/00; A61P31/00; A61P31/04; A61Q; A61Q11/00; A61Q5/00; A61Q5/02; C11D3/0005; C11D3/0078; C11D3/38636; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/938
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
56 words
An antibacterial composition having a pH between 6.5 and 8.5 in a 10% by weight solution in water at 20° C., comprising ruminant stomach lysozyme, and endo-β-N-acetylglucosaminidase or endoglycopeptidase in a weight ratio of from about 1:4 to 4:1; wherein said endo-β-N-acetylglucosaminodase is Endo-F, Endo-D or Endo-H, or said endoglycopepidase is PNGase F or PNGase A.
Claim 2
Dependent Claim
56 words
An antibacterial composition having a pH between 6.5 and 8.5 in a 10% by weight solution in water at 20° C., comprising ruminant stomach lysozyme, and endo-β-N-acetylglucosaminidase or endoglycopeptidase in a weight ratio of from about 1:4 to 4:1; wherein said endo-β-N-acetylglucosaminodase is Endo-F, Endo-D or Endo-H, or said endoglycopepidase is PNGase F or PNGase A.
Claim 3
Dependent Claim
19 words
The antimicrobial composition of claim 1 wherein said ruminant stomach lysozyme is one or more ruminant stomach lysozymes c.
Claim 4
Dependent Claim
25 words
The antimicrobial composition of claim 2 wherein the weight ratio of ruminant stomach lysozyme c to endo-β-N-acetylglucosaminidase or endoglycopeptidase is from about 2:1 to 1:2.
Claim 5
Dependent Claim
29 words
The antimicrobial composition of claim 2 comprising from about 1 to 1000 ppm of said ruminant stomach lysozyme c and from about 1 to 1200 ppm endo-β-N-acetylglucosaminidase or endoglycopeptidase.
Claim 6
Dependent Claim
15 words
The antimicrobial composition of claim 4 comprising bovine lysozyme c2 and Endo-H or PNGase F.
Claim 7
Dependent Claim
24 words
The antimicrobial composition of claim 5 comprising from about 50 to 400 ppm each of said bovine lysozyme and said Endo-H or PNGase F.
Claim 8
Dependent Claim
13 words
The antimicrobial composition of claim 6 comprising recombinant bovine lysozyme c2, and Endo-H.
Claim 9
Dependent Claim
23 words
The antimicrobial composition of claim 7 wherein the weight ratio of said recombinant bovine lysozyme to Endo-H is from about 2:1 to 1:2.
Claim 10
Dependent Claim
21 words
The antimicrobial composition of claim 8 comprising from 50 ppm each of Endo-H and said recombinant bovine lysozyme from Pichia pastoris.
Claim 11
Dependent Claim
29 words
The antimicrobial composition of claim 4 wherein said composition is selected from the following: mouthwash, denture cleaner, dentifrice, laundry detergent, preservative, contact lens cleaner, liquid soap, and skin cleanser.
Claim 12
Dependent Claim
29 words
The antimicrobial composition of claim 4 further comprising from about to 90 weight % detergent surfactant selected from the group consisting of anionic, nonionic, cationic, ampholytic and zwitterionic surfactants.
Claim 13
Dependent Claim
33 words
The antimicrobial composition of claim 5 wherein said composition is a mouthwash, denture cleaner or dentifrice comprising from about 1 to 150 ppm each of Endo-H or PNGase F and bovine lysozyme c2.
Claim 14
Dependent Claim
55 words
The antimicrobial composition of claim 7 wherein said composition is a liquid or granular laundry detergent comprising from about 2 to 250 ppm each of Endo-H and recombinant bovine lysozyme c2, and from about 5 to 50 weight % of detergent surfactant selected from the group consisting of anionic, nonionic, cationic, ampholytic and zwitterionic surfactants.
Claim 15
Dependent Claim
17 words
The antimicrobial composition of claim 6 wherein said composition is a preservative for a shampoo or cosmetic.
Claim 16
Dependent Claim
39 words
The antimicrobial composition of claim 6 wherein said composition is a liquid hand soap further comprising from about 10 to 40 weight % of detergent surfactant selected from the group consisting of anionic, nonionic, cationic, ampholytic and zwitterionic surfactants.
Claim 17
Dependent Claim
13 words
The antimicrobial composition of claim 9 wherein said composition is a skin cleaner.
Claim 18
Independent Claim
68 words
A method for the destruction or removal of microbes by application of a composition having a pH between 6.5 and 8.5 in a 10% by weight solution in water at 20° C. comprising ruminant stomach lysozyme and endo-β-N-acetylglucosaminidase or endoglycopeptidase in a weight ratio of from about 1:4 to 4:1, wherein said endo-β-N-acetyl glucosaminidase is Endo-F, Endo-D or Endo-H, or said endoglycopeptidase is PNGase F or PNGase A.
Claim 19
Independent Claim
68 words
A method for the destruction or removal of microbes by application of a composition having a pH between 6.5 and 8.5 in a 10% by weight solution in water at 20° C. comprising ruminant stomach lysozyme and endo-β-N-acetylglucosaminidase or endoglycopeptidase in a weight ratio of from about 1:4 to 4:1, wherein said endo-β-N-acetyl glucosaminidase is Endo-F, Endo-D or Endo-H, or said endoglycopeptidase is PNGase F or PNGase A.
Claim 20
Dependent Claim
13 words
The method of claim 17 wherein said composition comprises ruminant stomach lysozyme c.
Claim 21
Dependent Claim
30 words
The method of claim 18 wherein the weight ratio of said ruminant stomach lysozyme c to said endo-β-N-acetylglucosaminidase or said endoglycopeptidase in said composition is from about 2:1 to 1:2.
Claim 22
Dependent Claim
17 words
The method of claim 18 wherein said composition comprises bovine lysozyme c2, and Endo-H or PNGase F.
Claim 23
Dependent Claim
20 words
The method of claim 20 wherein said composition comprises Endo-H or PNGase F, and recombinant bovine lysozyme from Pichia pastoris.
Claim 24
Dependent Claim
35 words
The method of claim 21 for the destruction or removal of bacteria wherein said composition comprises from about 1 to 1200 ppm Endo-H and from about 1 to 1000 ppm of said recombinant bovine lysozyme.
Claim 25
Dependent Claim
34 words
The method of claim 22 for the destruction or removal of Staphylococcus aureus or Escherichia coli wherein said composition comprises from about 50 to 400 ppm each of said recombinant bovine lysozyme and Endo-H.
Claim 26
Dependent Claim
25 words
The method of claim 22 wherein the weight ratio of said recombinant bovine lysozyme to Endo-H in said composition is from about 1:2 to 2:1.
Claim 27
Dependent Claim
23 words
The method of claim 24 wherein said composition comprises from about 100 to 150 ppm each of Endo-H and said recombinant bovine lysozyme.
References Cited (Backward Citations)
GB1311375A
Cited
JPS4948825A
Cited
JPS55153709A
Cited
SU867738A1
Cited
DE2937964A1
Cited
JPS5775926A
Cited
US4355022A
Cited
GB2120140A
Cited
JPS5988086A
Cited
US4639375A
Cited
JPS6115827A
Cited
US4939123A
Cited
EP0192401A2
Cited
US4619825A
Cited
US4710313A
Cited
ZA867738B
Cited
JPS6244180A
Cited
EP0219220A1
Cited
JPS62248487A
Cited
JPS632911A
Cited
FR2600535A1
Cited
WO1988005992A2
Cited
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