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EP0192401A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Polypeptide product

Assignee: Novo Nordisk AS
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Filing: 1986-02-11
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Granted: —
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Expires: 2006-02-11
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Abstract

Abstract Novel, microbial α-galactosidase products which herein are designated a-galactomannangalactosidase, are able to split off D-galactopyranosyl groups from galactomannans without simultaneous degradation of the mannan backbone. The enzyme products can, for example, be prepared by Penicillium species. The novel enzyme products can be used even when the galactomannans are in a relatively high-concentration solution. Using the novel enzyme products, it is possible to prepare galactomannans having desired gelling capacities.

Inventors

  • 👤 Ruby Ione Nielsen
  • 👤 Martin Schülein

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-05 10:12
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2465

Claims Analysis

Total Claims: 11
Claim 1
Independent Claim 3 words
A microbial a-galactomannangalactosidase.
Claim 2
Dependent Claim 3 words
A microbial a-galactomannangalactosidase.
Claim 3
Dependent Claim 24 words
An α-galactomannangalactosidase product having at least about 250 GALU per gram of liquid or solid product form and being essentially free from endo-B-mannanase activity.
Claim 4
Dependent Claim 24 words
An α-galactomannangalactosidase product having at least about 250 GALU per gram of liquid or solid product form and being essentially free from endo-B-mannanase activity.
Claim 5
Dependent Claim 18 words
The a-galactomannangalactosidase product of Claim 2 wherein the endo-6-mannanase activity is less than about 0.001 EMANU per GALU.
Claim 6
Dependent Claim 38 words
The microbial a-galactomannangalactosidase elaborated extracellularly by cultivation of a microorganism strain productive of said enzyme selected from the group consisting of Penicillium chrysogenum, Penicillium islandicum and Penicillium notatum or mutants thereof and thereafter recovered from the culture broth.
Claim 7
Dependent Claim 38 words
The microbial a-galactomannangalactosidase elaborated extracellularly by cultivation of a microorganism strain productive of said enzyme selected from the group consisting of Penicillium chrysogenum, Penicillium islandicum and Penicillium notatum or mutants thereof and thereafter recovered from the culture broth.
Claim 8
Independent Claim 37 words
The method for producing a-galactomannangalactosidase which comprises cultivating by submerged cultivation an a-galactomannangalactosidase producing strain selected from the group consisting of Penicillium chrysogenum, Penicillium islandicum and Penicillium notatum of murants Phatect the enzyme from the culture medium.
Claim 9
Independent Claim 37 words
The method for producing a-galactomannangalactosidase which comprises cultivating by submerged cultivation an a-galactomannangalactosidase producing strain selected from the group consisting of Penicillium chrysogenum, Penicillium islandicum and Penicillium notatum of murants Phatect the enzyme from the culture medium.
Claim 10
Independent Claim 65 words
A method for modifying the galactomannan of guar gum, locust bean gum and the like which comprises treating the gum in solution with an a-galactomannangalactosidase product having at least about 250 GALU per gram thereof and less than about 0.01 EMANU of endo-3-mannanase per GALU until at least about 10% of the galactose content in the gum has split off and thereafter halting the treatment.
Claim 11
Independent Claim 65 words
A method for modifying the galactomannan of guar gum, locust bean gum and the like which comprises treating the gum in solution with an a-galactomannangalactosidase product having at least about 250 GALU per gram thereof and less than about 0.01 EMANU of endo-3-mannanase per GALU until at least about 10% of the galactose content in the gum has split off and thereafter halting the treatment.

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