US5019232A
Expired / Lapsed
US
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Medium for electrophoresis
Assignee: 3M Co
•
Filing: 1990-06-01
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Granted: 1991-05-28
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Expires: 2010-06-01
Abstract
Abstract A medium for electrophoresis comprises: (a) a polytetrafluoroethylene (PTFE) fibril matrix, and (b) particulate, electrically mobile ions, and sufficient liquid in the interstitial spaces of said matrix to allow for ion transport, the ratio of said particulate to PTFE being in the range of 99:1 to 4:1 by weight, and said ions being present in said liquid in an amount to provide a solution of concentration in the range of 1 to 1000 millimolar. Preferably the medium is self supporting.
Inventors
- 👤 Theresa J. Wilson
- 👤 Louis C. Haddad
- 👤 Donald F. Hagen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 10:05
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; B; B01; B01D; B01D57/00; B01D57/02
Claims Analysis
Total Claims: 37
Claim 1
Independent Claim
109 words
A medium for electrophoresis comprising (a) a polytetrafluoroethylene (PTFE) fibril matrix, and (b) particulate having an average size in the range of 1 to 600 micrometers electrically mobile ions, and sufficient liquid in the interstitial spaces of said matrix to allow for ion transport, the ratio of said particulate to PTFE being in the range of 99:1 to 4:1 by weight, and said ions being present in said liquid in an amount to provide a solution of concentration in the range of 1 to 1000 millimolar, and wherein almost all of said particulate are separate one from another and are isolated in cages or cage-like structures of PTFE microfibers.
Claim 2
Independent Claim
109 words
A medium for electrophoresis comprising (a) a polytetrafluoroethylene (PTFE) fibril matrix, and (b) particulate having an average size in the range of 1 to 600 micrometers electrically mobile ions, and sufficient liquid in the interstitial spaces of said matrix to allow for ion transport, the ratio of said particulate to PTFE being in the range of 99:1 to 4:1 by weight, and said ions being present in said liquid in an amount to provide a solution of concentration in the range of 1 to 1000 millimolar, and wherein almost all of said particulate are separate one from another and are isolated in cages or cage-like structures of PTFE microfibers.
Claim 3
Dependent Claim
11 words
The medium according to claim 1 wherein said particulate is polymeric.
Claim 4
Dependent Claim
11 words
The medium according to claim 2 wherein said particulate is polyacrylamide.
Claim 5
Dependent Claim
11 words
The medium according to claim 1 wherein said particulate is organic.
Claim 6
Dependent Claim
11 words
The medium according to claim 1 wherein said particulate is inorganic.
Claim 7
Dependent Claim
11 words
The medium according to claim 1 wherein said particulate is uncharged.
Claim 8
Dependent Claim
11 words
The medium according to claim 1 wherein said particulate is charged.
Claim 9
Dependent Claim
25 words
The medium according to claim 1 wherein said particulate is at least one of polyacrylamide, agarose, agar, end-capped cellulose, cellulose acetate, starch, polysaccharide, and sugar.
Claim 10
Dependent Claim
31 words
The medium according to claim 1 wherein said particulate is selected from the group consisting of agarose, agar, polyacrylates, polymethacrylates, styrene-divylbenzene copolymers, polyacrylamides, polyvinylacohol, polyvinylpyrrolidone, and particles coated with these materials.
Claim 11
Dependent Claim
13 words
The medium according to claim 1 further comprising at least one property modifier.
Claim 12
Dependent Claim
42 words
The medium according to claim 10 wherein said property modifier is present in the range of greater than 0 and up to 28.99 parts per part PTFE, provided that the total particulate does not exceed 99 parts particulate to 1 part PTFE.
Claim 13
Dependent Claim
45 words
The medium according to claim 10 wherein said modifier particles are selected from the group consisting of calcium carbonate, ammonium carbonate, kaolin, sugar, polyethylenes, polypropylenes, polymethacrylates, polyesters, polyamides, polyurethanes, polycarbonates, zeolites, cellulosics, silica, polysaccharide, vermiculite, clay, ceramics, chelating particles, and particles coated with these materials.
Claim 14
Dependent Claim
11 words
The medium according to claim 1 wherein said liquid is aqueous-based.
Claim 15
Dependent Claim
11 words
The medium according to claim 1 wherein said liquid is organic.
Claim 16
Dependent Claim
29 words
The medium according to claim 13 wherein said aqueous-based liquid comprises at least one of an alcohol, an acid, a base, or a combination of water and organic liquid.
Claim 17
Dependent Claim
11 words
The medium according to claim 13 wherein said liquid is water.
Claim 18
Dependent Claim
23 words
The medium according to claim 1 wherein said electrically mobile ionic compound is selected from the group consisting of acids, bases, and salts.
Claim 19
Dependent Claim
22 words
The medium according to claim 1 wherein said solution has a mobile ionic concentration in the range of 50 to 250 millimolar.
Claim 20
Dependent Claim
11 words
The medium according to claim 1 which is a separating gel.
Claim 21
Dependent Claim
11 words
The medium according to claim 1 which is a stacking gel.
Claim 22
Dependent Claim
23 words
The medium according to claim 10 which is a combination of stacking gel and a separating gel to provide a discontinuous electrophoretic medium.
Claim 23
Dependent Claim
12 words
The medium according to claim 1 which is a gel electrophoretic medium.
Claim 24
Dependent Claim
9 words
The medium according to claim 1 which is self-supporting.
Claim 25
Independent Claim
101 words
A medium for electrophoresis comprising (a) a polytetrafluoroethylene fibril matrix, and (b) polyacrylamide particulate having an average size in the range of 1 to 100 micrometers, electrically mobile ions, and sufficient water in the interstitial spaces of said matrix to allow for ion transport, the ratio of particulate to polytetrafluoroethylene being in the range of 99:1 to 4:1, and said ions being present in said liquid in an amount to provide a solution of 1 to 1000 millimolar, and wherein almost all of said particulate are separate one from another and are isolated in cages or cage-like structures of PTFE microfibers.
Claim 26
Independent Claim
101 words
A medium for electrophoresis comprising (a) a polytetrafluoroethylene fibril matrix, and (b) polyacrylamide particulate having an average size in the range of 1 to 100 micrometers, electrically mobile ions, and sufficient water in the interstitial spaces of said matrix to allow for ion transport, the ratio of particulate to polytetrafluoroethylene being in the range of 99:1 to 4:1, and said ions being present in said liquid in an amount to provide a solution of 1 to 1000 millimolar, and wherein almost all of said particulate are separate one from another and are isolated in cages or cage-like structures of PTFE microfibers.
Claim 27
Independent Claim
151 words
A method for electrophoresis comprising the steps: (a) applying a sample comprising one or more components onto a medium comprising a polytetrafluoroethylene fibril matrix and at least one of non-swellable and swellable particulate having an average size in the range of 1 to 600 micrometers, electrically mobile ions, and sufficient liquid in the interstitial spaces of said matrix to allow for ion transport, the ratio of particulate to polytetrafluoroethylene being in the range of 99:1 to 4:1 by weight, said ions being present in said liquid in an amount to provide a solution of concentration in the range of 1 to 1000 millimolar, and wherein almost all of said particulate are separate one from another and are isolated in cages or cage-like structures of PTFE microfibers, (b) applying an electric current to said medium of sufficient strength and for a time sufficient to effect separation of the components of said sample.
Claim 28
Independent Claim
151 words
A method for electrophoresis comprising the steps: (a) applying a sample comprising one or more components onto a medium comprising a polytetrafluoroethylene fibril matrix and at least one of non-swellable and swellable particulate having an average size in the range of 1 to 600 micrometers, electrically mobile ions, and sufficient liquid in the interstitial spaces of said matrix to allow for ion transport, the ratio of particulate to polytetrafluoroethylene being in the range of 99:1 to 4:1 by weight, said ions being present in said liquid in an amount to provide a solution of concentration in the range of 1 to 1000 millimolar, and wherein almost all of said particulate are separate one from another and are isolated in cages or cage-like structures of PTFE microfibers, (b) applying an electric current to said medium of sufficient strength and for a time sufficient to effect separation of the components of said sample.
Claim 29
Dependent Claim
28 words
The method according to claim 25 wherein said medium comprises particulate which is at least one of acrylamide, polyacrylamide, agarose, agar, end-capped cellulose, cellulose acetate, starch, and sugar.
Claim 30
Dependent Claim
17 words
The method according to claim 25 further comprising the step of subjecting said separated components to electroblotting.
Claim 31
Dependent Claim
19 words
The method according to claim 25 further comprising the step of individually cutting each separated component from said medium.
Claim 32
Dependent Claim
19 words
The method according to claim 27 further comprising the step of individually cutting each separated component from said medium.
Claim 33
Dependent Claim
11 words
The method according to claim 25 wherein said liquid is aqueous-based.
Claim 34
Dependent Claim
11 words
The method according to claim 25 wherein said liquid is organic.
Claim 35
Dependent Claim
21 words
The medium according to claim 1 wherein said particulate has an average size in the range of 1 to 100 micrometers.
Claim 36
Dependent Claim
21 words
The method according to claim 25 wherein said particulate has an average size in the range of 1 to 100 micrometers.
Claim 37
Dependent Claim
20 words
The medium according to claim 1 which has been dried to provide a medium for electrophoresis which is uniformly porous.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1994-05-27 | 1994-11-26 | 1995-05-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-05-27 | 1998-11-26 | 1999-05-28 | $3760.00 | Window Closed / Lapsed |
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