US4657656A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Polyacrylamide medium for electrophoresis having improved elasticity
Assignee: Fujifilm Holdings Corp
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Filing: 1984-09-14
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Granted: 1987-04-14
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Expires: 2004-09-14
Abstract
Abstract In a medium for electrophoresis comprising a polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, and a modifier, the improvement in which the medium contains a water-soluble polymer.
Inventors
- 👤 Masashi Ogawa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 00:18
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
108 words
In a medium for electrophoresis comprising a polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water and a modifier selected from the group consisting of urea and formamide, the improvement which comprises said medium containing a water-soluble polymer having a molecular weight in the range of 10,000 to 1,000,000 selected from the group consisting of an addition type polymer and a polycondensation type polymer, said water-soluble polymer being incorporated into the medium in the form of a polymer in an amount of 1 to 50 wt.%, based on the total weight of the acrylamide compound and crosslinking agent.
Claim 2
Dependent Claim
108 words
In a medium for electrophoresis comprising a polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water and a modifier selected from the group consisting of urea and formamide, the improvement which comprises said medium containing a water-soluble polymer having a molecular weight in the range of 10,000 to 1,000,000 selected from the group consisting of an addition type polymer and a polycondensation type polymer, said water-soluble polymer being incorporated into the medium in the form of a polymer in an amount of 1 to 50 wt.%, based on the total weight of the acrylamide compound and crosslinking agent.
Claim 3
Dependent Claim
44 words
The medium for electrophoresis as claimed in claim 1 wherein said water-soluble polymer is incorporated into the medium in the form of a polymer in an amount of 5 to 30 wt.% based on the total weight of the acrylamide compound and crosslinking agent.
Claim 4
Dependent Claim
31 words
The medium for electrophoresis as claimed in claim 1 or 2 wherein said water-soluble polymer is selected from the group consisting of polyvinyl alcohol, polyvinylpyrrolidone, polyacrylamide, polyethylene glycol and polypropylene glycol.
Claim 5
Dependent Claim
26 words
The medium for electrophoresis as claimed in claim 1 or 2 wherein said water-soluble polymer is selected from the group consisting of polyacrylamide and polyethylene glycol.
Claim 6
Dependent Claim
116 words
In a medium for electrophoresis comprising a polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water and a modifier selected from the group consisting of urea and formamide, the improvement which comprises said medium containing a water-soluble polymer having a molecular weight in the range of 10,000 to 1,000,000 selected from the group consisting of an addition type polymer and a polycondensation type polymer, said water-soluble polymer being incorporated into the medium in the form of a polymer in an amount of 1 to 50 wt.%, based on the total weight of the acrylamide compound and crosslinking agent, said polymer being homogeneously dispersed in said gel.
Claim 7
Dependent Claim
116 words
In a medium for electrophoresis comprising a polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water and a modifier selected from the group consisting of urea and formamide, the improvement which comprises said medium containing a water-soluble polymer having a molecular weight in the range of 10,000 to 1,000,000 selected from the group consisting of an addition type polymer and a polycondensation type polymer, said water-soluble polymer being incorporated into the medium in the form of a polymer in an amount of 1 to 50 wt.%, based on the total weight of the acrylamide compound and crosslinking agent, said polymer being homogeneously dispersed in said gel.
References Cited (Backward Citations)
US4432881A
Cited
US4415428A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-04-13 | 1990-10-13 | 1991-04-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-04-13 | 1994-10-13 | 1995-04-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-04-13 | 1998-10-13 | 1999-04-14 | $7700.00 | Window Closed / Lapsed |