US4718998A
Expired / Lapsed
US
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Element for electrophoresis
Assignee: Fujifilm Holdings Corp
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Filing: 1987-05-19
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Granted: 1988-01-12
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Expires: 2007-05-19
Abstract
Abstract An element for electrophoresis suitably employable for analysis of biopolymers such as proteins, as well as for determination of base sequence of DNA, RNA, their fragments, and their derivatives, which comprises a plastic film, a layer for electrophoresis comprising an aqueous polyacrylamide gel, an adhesive layer containing gelatin and optionally a film-forming polymer and/or fine particles, and a plastic film.
Inventors
- 👤 Masashi Ogawa
- 👤 Naohiko Sugimoto
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 00:59
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; Y; Y10; Y10T; Y10T428/00; Y10T428/31504; Y10T428/31507; Y10T428/31786; Y10T428/31797; Y10T428/31855; Y10T428/31909; Y10T428/31913; Y10T428/3192
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
52 words
An element for electrophoresis comprising: (I) a plastic film; (II) a layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water; (III) an adhesive layer containing gelatin; and (IV) a plastic film, which are superposed in order.
Claim 2
Dependent Claim
52 words
An element for electrophoresis comprising: (I) a plastic film; (II) a layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water; (III) an adhesive layer containing gelatin; and (IV) a plastic film, which are superposed in order.
Claim 3
Dependent Claim
21 words
The element for electrophoresis as claimed in claim 1, wherein said gelatin contained in the adhesive layer is a lime-treated gelatin.
Claim 4
Dependent Claim
17 words
The element for electrophoresis as claimed in claim 1, wherein the adhesive layer consisting essentially of gelatin.
Claim 5
Dependent Claim
27 words
The element for electrophoresis as claimed in claim 1, wherein said adhesive layer contains at least 5 wt. % of gelatin and further contains a film-forming material.
Claim 6
Dependent Claim
53 words
The element for electrophoresis as claimed in claim 1, wherein said adhesive layer contains at least 5 wt. % of gelatin and further contains a material selected from the group consisting of cellulose derivatives, dextran, agarose, polyacrylamide, polyvinyl alcohol esters, polyacrylate esters, pullulan, pullulan derivatives, polyvinylidene chloride, vinylidene chloride latex and water glass.
Claim 7
Dependent Claim
29 words
The element for electrophoresis as claimed in claim 1, wherein said adhesive layer contains at least 5 wt. % of gelatin and further contains a filler of fine particles.
Claim 8
Dependent Claim
29 words
The element for electrophoresis as claimed in claim 1, wherein said adhesive layer contains at least 5 wt. % of gelatin and further contains colloidal silica or methanol silica.
Claim 9
Dependent Claim
22 words
The element for electrophoresis as claimed in claim 1, wherein said medium layer contains a compound having at least one carbamoyl group.
Claim 10
Dependent Claim
17 words
The element for electrophoresis as claimed in claim 1, wherein said medium layer contains urea or formamide.
Claim 11
Dependent Claim
19 words
The element for electrophoresis as claimed in claim 1, wherein said medium layer contains a water-soluble polymer and agarose.
Claim 12
Dependent Claim
17 words
The element for electrophoresis as claimed in claim 1, wherein said medium layer contains an anionic surfactant.
Claim 13
Dependent Claim
16 words
The element for electrophoresis as claimed in claim 1, wherein said medium layer contains an alkylsulfate.
Claim 14
Dependent Claim
21 words
The element for electrophoresis as claimed in claim 1, wherein the plastic film (I) is thicker than the plastic film (IV).
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-01-11 | 1991-07-13 | 1992-01-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-01-11 | 1995-07-13 | 1996-01-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-01-11 | 1999-07-13 | 2000-01-12 | $7700.00 | Window Closed / Lapsed |