Patent US5004547A successfully refreshed from Google Patents.
US5004547A
Expired / Lapsed
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Method and apparatus for bioaffinity separation
Assignee: Technion Research and Development Foundation Ltd
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Filing: 1989-07-05
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Granted: 1991-04-02
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Expires: 2009-07-05
Abstract
Abstract Bioaffinity separation is carried out in a cascade of column segments under control of a computer which selectively connects each column segment to a collector so that the individual bioactive components which are selectively retained by a biospecific sorbent can be individually collected. The volume and flow rate of eluate collected following each segment serve as the measure for the addition of makeup eluant between that column segment of the cascade and the next column segment.
Inventors
- 👤 Hay Grunfeld
- 👤 Noah Lotan
- 👤 Samuel Sideman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 04:03
CPC Classifications
B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01D15/10; B01D15/18; B01D15/1807; B01D15/1864; B01D15/1871; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
229 words
A method of bioaffinity separation for recovering simultaneously a plurality of bioactive components of a liquid feed which comprises the steps of: (a) introducing a liquid feed containing a plurality of bioactive components into a first of a plurality of bioaffinity retention stages in cascade constituting respective column segments of a separation column packed with sorbent containing at least one biospecific substance so that said segments are loaded with said substance to different degrees along the length of the column; (b) passing an eluant through said column segments in cascade to elute simultaneously and concurrently the components of said feed from the biospecific substance in each column segment; (c) automatically connecting an outlet from each column segment to a respective collector upon passage of faster-eluting components of said feed to a successive column segment of the cascade to recover in each collector a slower-eluting component of said feed in an eluate; (d) measuring the volume and flow rate of said eluate collected in each collector of a respective column segment and automatically feeding a corresponding makeup stream of said eluant to a successive column segment; and (e) controlling the automatic connection of said outlets from said column segments to the respective collectors with preprogrammed control of timing subsequent to the inception of elution by a computer for selective recovery of said components of said feed at the respective collectors.
Claim 2
Independent Claim
229 words
A method of bioaffinity separation for recovering simultaneously a plurality of bioactive components of a liquid feed which comprises the steps of: (a) introducing a liquid feed containing a plurality of bioactive components into a first of a plurality of bioaffinity retention stages in cascade constituting respective column segments of a separation column packed with sorbent containing at least one biospecific substance so that said segments are loaded with said substance to different degrees along the length of the column; (b) passing an eluant through said column segments in cascade to elute simultaneously and concurrently the components of said feed from the biospecific substance in each column segment; (c) automatically connecting an outlet from each column segment to a respective collector upon passage of faster-eluting components of said feed to a successive column segment of the cascade to recover in each collector a slower-eluting component of said feed in an eluate; (d) measuring the volume and flow rate of said eluate collected in each collector of a respective column segment and automatically feeding a corresponding makeup stream of said eluant to a successive column segment; and (e) controlling the automatic connection of said outlets from said column segments to the respective collectors with preprogrammed control of timing subsequent to the inception of elution by a computer for selective recovery of said components of said feed at the respective collectors.
Claim 3
Dependent Claim
65 words
The method defined in claim 1, wherein said liquid feed contains a solvent forming a mobile phase in which said components are entrained, and step (a) comprises: (a 1 ) initially equilibrating all of said segments with said solvent; and (a 2 ) then under the control of said computer, injecting into said first of said column segments, a pulsed quantity of said liquid feed.
Claim 4
Dependent Claim
22 words
The method defined in claim 1, further comprising the step of charging all of said segments with the same solid biospecific sorbent.
Claim 5
Dependent Claim
25 words
The method defined in claim 3, wherein said biospecific sorbent is agarose gel charged with benzamidine and said feed contains trypsin, said eluant containing p-aminobenzamidine.
Claim 6
Dependent Claim
35 words
The method defined in claim 1, wherein said column segments are packed with beads of agarose gel charged with a biospecific substance attached with different degrees of substitution for at least some of said segments.
Claim 7
Dependent Claim
12 words
The method defined in claim 5, wherein said biospecific substance is p-aminobenzamidine.
Claim 8
Dependent Claim
74 words
The method defined in claim 1 wherein the automatic connection of said outlets from said column segments to the respective collectors is affected by: analyzing an outflow from each of said column segments in terms of flow rate, time and presence of a specific one of said bioactive components; processing data representing each analysis of said outflow from each column segment; and controlling the automatic connections by the computer responding to the processed data.
Claim 9
Independent Claim
301 words
An apparatus for recovering simultaneously a plurality of bioactive components of a liquid feed by bioaffinity separation, which comprises: a plurality of bioaffinity retention stages in cascade constituting respective column segments of a separation column containing at least one biospecific sorbent; respective collectors connectable with outlets of each of said column segments; means for introducing a liquid feed containing a plurality of bioactive components into a first of said plurality of said bioaffinity retention stages in cascade constituting said respective column segments of said separation column so that said segments are loaded with said biospecific substance to different degrees along the length of the column; means for passing an eluant through said column segments in cascade simultaneously and concurrently to elute the components of said feed from the biospecific sorbent in each column segment and afford simultaneous recovery of said components; means for automatically connecting an outlet from each column segment to a respective one of said collectors upon passage of faster-eluting components of said feed to a successive column segment of the cascade to recover in each collector a slower-eluting component of said feed in an eluate; and means for measuring the volume and flow rate of said eluate collected in each collector of a respective column segment and automatically feeding a corresponding makeup stream of said eluant to a successive column segment, said means for automatically connecting an outlet from each column segment to a respective one of said collectors including an electrically controlled valve for selectively connecting each of said outlets with the successive column segment and with the respective collector, and a computer connected to said valves and with preprogrammed control of timing of operation of said valves subsequent to the inception of elution for selective recovery of said components of said feed at the respective collectors.
Claim 10
Independent Claim
301 words
An apparatus for recovering simultaneously a plurality of bioactive components of a liquid feed by bioaffinity separation, which comprises: a plurality of bioaffinity retention stages in cascade constituting respective column segments of a separation column containing at least one biospecific sorbent; respective collectors connectable with outlets of each of said column segments; means for introducing a liquid feed containing a plurality of bioactive components into a first of said plurality of said bioaffinity retention stages in cascade constituting said respective column segments of said separation column so that said segments are loaded with said biospecific substance to different degrees along the length of the column; means for passing an eluant through said column segments in cascade simultaneously and concurrently to elute the components of said feed from the biospecific sorbent in each column segment and afford simultaneous recovery of said components; means for automatically connecting an outlet from each column segment to a respective one of said collectors upon passage of faster-eluting components of said feed to a successive column segment of the cascade to recover in each collector a slower-eluting component of said feed in an eluate; and means for measuring the volume and flow rate of said eluate collected in each collector of a respective column segment and automatically feeding a corresponding makeup stream of said eluant to a successive column segment, said means for automatically connecting an outlet from each column segment to a respective one of said collectors including an electrically controlled valve for selectively connecting each of said outlets with the successive column segment and with the respective collector, and a computer connected to said valves and with preprogrammed control of timing of operation of said valves subsequent to the inception of elution for selective recovery of said components of said feed at the respective collectors.
Claim 11
Dependent Claim
47 words
The apparatus defined in claim 8, wherein said means for measuring the volume and flow rate of said eluate collected in each collector are connected to said computer and said computer effects automatic feed of said corresponding make-up stream of said eluant to a successive column segment.
Claim 12
Dependent Claim
53 words
The apparatus defined in claim 8, wherein said means for introducing a liquid feed containing a plurality of bioactive components into a first of said plurality of said bioaffinity retention stages includes a vessel containing said feed and a pump controlled by said computer and connected to said first of said column segments.
Claim 13
Dependent Claim
41 words
The apparatus defined in claim 8, wherein said means for passing an eluant through said column segments in cascade includes a vessel containing said eluant and a pump controlled by said computer and connecting said vessel with said first column segment.
Claim 14
Dependent Claim
82 words
The apparatus defined in claim 8, wherein the means for automatically connecting an outlet from each column segment to a respective one of said collectors includes: means for analyzing an outflow from each of said column segments in terms of flow rate, time and presence of a specific one said bioactive components; and means for processing data resulting from the analysis of the outflow from each column segment by said means for analyzing, said computer being connected to said means for processing.
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