US3917527A
Expired / Lapsed
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Hydrophobic chromatography
Assignee: Yeda Research and Development Co Ltd
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Filing: 1973-05-14
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Granted: 1975-11-04
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Expires: 1993-05-14
Abstract
Abstract Biomolecules such as peptides, vitamins, hormones, lipids, proteins and enzymes as well as membranes, cells and the like are separated and purified using one or more members of a homologous series of chromatographic adsorbents having hydrocarbon arms of predetermined length attached thereto.
Inventors
- 👤 Shmuel Shaltiel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:37
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/10; B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/32; B01D15/325; B01D15/327; B01J; B01J20/00; B01J20/281; B01J20/286; B01J20/30; B01J20/32; B01J20/3202; B01J20/3206; B01J20/3208; B01J20/3212; B01J20/3214; B01J20/3217; B01J20/3219; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3248; B01J20/3253; B01J20/3291; B01J20/3293; B01J2220/00; B01J2220/50; B01J2220/54; B01J2220/58; Y; Y10; Y10S; Y10S435/00; Y10S435/80; Y10S435/803; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
9 words
The method of claim 1, wherein A is agarose.
Claim 2
Dependent Claim
25 words
The method of claim 2, wherein said radicals are selected from the group consisting of -NH2, -SO3H, -CONH2, -PO4H2, -OH, -SH, imidazole and phenolic groups.
Claim 3
Dependent Claim
18 words
The method of claim 3, wherein B is selected from the group consisting of -NH-, -S- and -COO-.
Claim 4
Dependent Claim
15 words
The method of claim 4, wherein C is alkyl, aryl, aralkyl, alkylaryl or alkyl aralkyl.
Claim 5
Dependent Claim
16 words
The method of claim 1, wherein A is agarose, B is -NH-, and C is alkyl.
Claim 6
Dependent Claim
9 words
The method of claim 6, wherein D is H.
Claim 7
Dependent Claim
20 words
The method of claim 6, wherein D is NH2 and wherein said alkyl group contains one to 12 carbon atoms.
Claim 8
Independent Claim
131 words
A method for determining which member of a homologous series of adsorbents each of which is a water-insoluble carrier having an arm attached of the formula A-B-C-D, wherein A is a water-insoluble carrier, B-C-D represents the arm and individually B is a covalent linking group, C is a hydrocarbon group, and D is a terminal group selected from the group consisting of hydrogen, ionic and non-ionic radicals, said series differing in the number of carbon atoms in C, will selectively and reversibly bind a given biomolecule which comprises contracting individual portions of a solution containing said biomolecule with individual members of said series, identifying which members of said series bind said biomolecule, changing the eluting conditions, and identifying which member of said series will release said biomolecule in a non-denatured form.
Claim 9
Dependent Claim
16 words
The method of claim 9, wherein A is agarose, B is -NH, and C is alkyl.
Claim 10
Dependent Claim
9 words
The method of claim 10, wherein D is H.
Claim 11
Dependent Claim
20 words
The method of claim 10, wherein D is NH2 and wherein said alkyl group contains one to 12 carbon atoms.
Claim 12
Dependent Claim
91 words
A package containing a series of chromatographic columns, each containing a reagent which is a water-insoluble carrier having an arm attached thereto of the formula A-B-C-D as the adsorbent wherein A is a water-insoluble carrier, B-C-D represents the arm and individually B is a covalent linking group, C is a hydrocarbon group, and D is a terminal group selected from the group consisting of hydrogen, ionic radicals and non-ionic radicals, the reagents in said columns differing in the number of carbon atoms in C and forming a homologous series of reactants.
Claim 13
Dependent Claim
9 words
The package of claim 13, wherein A is agarose.
Claim 14
Dependent Claim
25 words
The package of claim 14, wherein said radicals are selected from the group consisting of -NH2, -SO3H, -PO4H2, -OH, -SH, CONH2, imidazole and phenolic groups.
Claim 15
Dependent Claim
18 words
The package of claim 15, wherein B is selected from the group consisting of -NH-, -S- and -COO-.
Claim 16
Dependent Claim
15 words
The package of claim 16, wherein C is alkyl, aryl, aralkyl, alkylaryl or alkyl aralkyl.
Claim 17
Dependent Claim
16 words
The package of claim 13, wherein A is agarose, B is -NH-, and C is alkyl.
Claim 18
Dependent Claim
9 words
The package of claim 18, wherein D is H.
Claim 19
Dependent Claim
20 words
The package of claim 18, wherein D is NH2 and wherein said alkyl group contains one to 12 carbon atoms.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-11-03 | 1979-05-05 | 1979-11-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-11-03 | 1983-05-05 | 1983-11-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-11-03 | 1987-05-05 | 1987-11-04 | $7700.00 | Window Closed / Lapsed |