Process of determining hemoglobin separated from peroxidase inhibitors in urine

Claim 1

A METHOD OF SEPARATING HEMOGLOBIN FROM PEROXIDASE INHIBITORS IN URINE CONTAINING THE SAME, COMPRISING ADDING TO A SPECIMEN OF SAID URINE A QUANTITY OF SERUM HAPTOGLOBIN AT LEAST EQUIVALENT STOICHIOMETRICALLY TO THE MAXIMUM AMOUNT OF HEMOGLOBIN TO BE SEPARATED, WHEREBY A COMPLEX IS FORMED BETWEEN SAID HEMOGLOBIN AND SAID HAPTOGLOBIN, INTRODUCING THE RESULTING URINE SPECIMEN ONTO A BED OF FINELY DIVIDED PARTICLES OF GEL CONSISTING ESSENTIALLY OF INSOLUBLE, HYDROPHILIC, DEXTRAN CROSS-LINKED WITH EPICHLOROHYDRIN AND HAVING A WATER-REGAIN VALUE OF ABOUT FROM 2.5 TO 7.5 THE GEL PARTICLES OF SAID BED BEING ENTIRELY SWOLLEN WITH AN AQUEOUS ELUANT AND THE SPACES BETWEEN SAID PARTICLES BEING FILLED WITH SAID ELUANT, SAID BED HAVING A HEIGHT DIAMETER RATIO OF AT LEAST ABOUT 10:1 THE VOLUME OF SAID URINE SPECIMEN BEING NOT MORE THAN ABOUT THE INBIBED VOLUME OF SAID GEL PARTICLES, AFTER SAID URINE SPECIMEN HAS ENTERED SAID BED AND WHILE THE LEVEL OF SAID URINE SPECIMEN IS SUBSTANTIALLY COINCIDENT WITH THE TOP OF SAID BED COMMENCING ELUTION OF THE LATTER BY PASSING AN AQUEOUS ELUTING LIQUID THERETHROUGH, CONTINUING SAID ELUTION UNTIL THE QUANTITY OF SAID ELUTING LIQUID USED FOR ELUTION EXCEED THE VOID VOLUME OF SAID BED, COLLECTING AS SEPARATE QUANTITIES A PLURALITY OF FRACTIONS OF EFFLUENT FROM THE BOTTOM OF SAID BED, THE VOLUME OF EACH OF SAID FRACTIONS BEING SMALLER THAN THE VOLUME OF SAID URINE SPECIMEN, AND TESTING SAID FRACTIONS FOR THE PRESENCE THEREIN OF SAID HEMOGLOBIN-HAPTOGLOBIN COMPLEX.