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Patent US4997914A successfully refreshed from Google Patents.
US4997914A
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Method for separating and purifying lactoferrin from milk by use of sulfuric ester
Assignee: Snow Brand Milk Products Co Ltd
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Filing: 1988-04-07
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Granted: 1991-03-05
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Expires: 2008-04-07
Abstract
Abstract A method for separating and purifying lactoferrin from milk is disclosed, which method comprises the steps of bringing raw milk containing lactoferrin into contact with a sulfuric ester of a crosslinked polysaccharide so that lactoferrin may be adsorbed by the sulfuric ester, and then eluting the adsorbed lactoferrin. The elution of the adsorbed lactoferrin is preferably conducted by the use of a buffer solution containing a 0.4-1.5 M aqueous sodium chloride solution.
Inventors
- 👤 Hiroshi Kawakami
- 👤 Morimasa Tanimoto
- 👤 Shunichi Dousako
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 20:17
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/79; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/832
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
44 words
A method for separating and purifying lactoferrin from raw milk, comprising the steps of: (a) contacting said raw milk with a resin comprising a sulfuric ester of a cross-linked polysaccharide in order to adsorb lactoferrin onto said resin, and (b) eluting said adsorbed lactoferrin.
Claim 2
Independent Claim
44 words
A method for separating and purifying lactoferrin from raw milk, comprising the steps of: (a) contacting said raw milk with a resin comprising a sulfuric ester of a cross-linked polysaccharide in order to adsorb lactoferrin onto said resin, and (b) eluting said adsorbed lactoferrin.
Claim 3
Dependent Claim
30 words
A method according to claim 1 wherein said contact is achieved by passing said raw milk containing lactoferrin through an affinity column comprising said sulfuric ester of said crosslinked polysaccharide.
Claim 4
Dependent Claim
37 words
A method according to claim 1 wherein said adsorbed lactoferrin is eluted with a buffer solution containing a 0.4-1.5M aqueous sodium chloride solution and is then subjected to electrodialysis or reverse osmosis to desalt the lactoferrin solution.
Claim 5
Dependent Claim
37 words
A method according to claim 2 wherein said adsorbed lactoferrin is eluted with a buffer solution containing a 0.4-1.5M aqueous sodium chloride solution and is then subjected to electrodialysis or reverse osmosis to desalt the lactoferrin solution.
Claim 6
Dependent Claim
14 words
A method according to claim 1 wherein said crosslinked polysaccharide is cellulose or chitin.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-03-04 | 1994-09-03 | 1995-03-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-03-04 | 1998-09-03 | 1999-03-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-03-04 | 2002-09-03 | 2003-03-05 | $7700.00 | Window Closed / Lapsed |