US4436658A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process of extraction of lactoferrine and immunoglobulins of milk
Assignee: Societe National Elf Aquitaine
•
Filing: 1982-05-12
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Granted: 1984-03-13
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Expires: 2002-05-12
Abstract
Abstract From an aqueous medium derived from milk substantially free from caseins, proteins are extracted, particularly lactoferrine and immunoglobulins, by adsorption on a solid support. The aqueous medium is weakly basic, while elution is effected by means of an acid solution.
Inventors
- 👤 Andre Peyrouset
- 👤 Francois Spring
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 20:27
CPC Classifications
A; A23; A23J; A23J1/00; A23J1/20; A23J1/205; A23V; A23V2002/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/832
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
89 words
In a process of extraction of iron-fixing milk proteins, from an aqueous medium substantially free from caseins by adsorption on a solid support followed by elution of the adsorbed proteins by means of an acid solution, the improvement which comprises effecting the adsorption in a weakly basic medium at a pH of 7.7 to 8.8 on pulverulent silica having a particle size from 5 um to 5 mm, a specific surface of about 5 to 150 m 2 /g and a pore diameter of from 25 to 250 nm.
Claim 2
Dependent Claim
89 words
In a process of extraction of iron-fixing milk proteins, from an aqueous medium substantially free from caseins by adsorption on a solid support followed by elution of the adsorbed proteins by means of an acid solution, the improvement which comprises effecting the adsorption in a weakly basic medium at a pH of 7.7 to 8.8 on pulverulent silica having a particle size from 5 um to 5 mm, a specific surface of about 5 to 150 m 2 /g and a pore diameter of from 25 to 250 nm.
Claim 3
Dependent Claim
27 words
Process according to claim 1, where the elution takes place at a pH below about 4 and the eluant contains a soluble salt increasing its ionic force.
Claim 4
Dependent Claim
26 words
Process according to claim 1, in which the elution is followed by treatment with a basic solution and recovery of the immunoglobulins and lactoferrine thus liberated.
Claim 5
Dependent Claim
17 words
Process according to claim 1, where the iron-fixing proteins comprise lactoferrine in a liquid derived from milk.
Claim 6
Dependent Claim
22 words
Process according to claim 4, wherein the immunoglobulins and/or other proteins are recovered from the liquid remaining after adsorption of the lactoferrine.
Claim 7
Dependent Claim
22 words
Process according to claim 1, wherein the pH of the medium subjected to adsorption is in the range from 7.9 to 8.5.
Claim 8
Dependent Claim
17 words
Process according to claim 6, where the silica particle size range is from 10 to 500 um.
References Cited (Backward Citations)
US3234199A
Cited
US3487064A
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US3547900A
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US3969337A
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US4018752A
Cited
US4100149A
Cited
US4229342A
Cited
US4265924A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-03-13 | 1987-09-12 | 1988-03-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-03-13 | 1991-09-12 | 1992-03-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-03-13 | 1995-09-12 | 1996-03-13 | $7700.00 | Window Closed / Lapsed |