US4100149A
Expired / Lapsed
US
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Method of separating proteins by ion exchange
Assignee: Rhone Poulenc Industries SA
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Filing: 1976-08-16
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Granted: 1978-07-11
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Expires: 1996-08-16
Abstract
Abstract A method of separating proteins by ion exchange comprising putting a solution of proteins into contact with an ion exchange resin consisting of a porous inorganic carrier coated with a cross-linked polymer containing or carrying anion exchanging groups -- tertiary amines or salts of quaternary ammonium -- or cation exchanging groups and application of the process to the food, pharmaceutical and veterinary field.
Inventors
- 👤 Francois Meiller
- 👤 Bernard Mirabel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 00:37
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2462; A; A23; A23C; A23C9/00; A23C9/14; A23C9/146; A23C9/1465; A23J; A23J1/00; A23J1/001; A23J1/08; A23J1/16; B; B01; B01J; B01J47/00; B01J47/014; B01J47/016; C07; C07K; C07K1/00; C07K1/14; C07K1/16; C07K1/18; C07K16/00; C07K16/06; C07K16/065; C12H; C12H1/00; C12H1/02; C12H1/04; C12H1/0432; Y; Y10; Y10S; Y10S526/00; Y10S526/936; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/832; Y10S530/859; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2991; Y10T428/2998
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
120 words
A method of separating proteins from solution, comprising putting a solution of proteins into contact with an ion exchange resin, comprising a porous inorganic carrier with a particle size from 4 μm to 5 mm, a specific surface area of approximately 5 to 150 m 2 /g, a pore diameter of 500 to 2500 A and a pore volume of 0.4 to 2 ml/g, coated with less than 15 mg/m 2 of a film of cross-linked polymer, containing or carrying either tertiary amines or quaternary ammonium salts, as anion exchanging groups, or acid functions as cationic exchanging groups, wherein at least one protein becoming fixed onto the ion exchange resin, and eluting the fixed protein(s) from the ion exchange resin.
Claim 2
Independent Claim
120 words
A method of separating proteins from solution, comprising putting a solution of proteins into contact with an ion exchange resin, comprising a porous inorganic carrier with a particle size from 4 μm to 5 mm, a specific surface area of approximately 5 to 150 m 2 /g, a pore diameter of 500 to 2500 A and a pore volume of 0.4 to 2 ml/g, coated with less than 15 mg/m 2 of a film of cross-linked polymer, containing or carrying either tertiary amines or quaternary ammonium salts, as anion exchanging groups, or acid functions as cationic exchanging groups, wherein at least one protein becoming fixed onto the ion exchange resin, and eluting the fixed protein(s) from the ion exchange resin.
Claim 3
Dependent Claim
24 words
The method of claim 1, in which the inorganic carrier is selected from the group consisting of metallic oxide, titanium oxide, aluminas, and silicas.
Claim 4
Dependent Claim
58 words
The method of claim 1, in which the anion-exchanging groups are represented by formulae selected from the group consisting of ##STR5## in which R, which may be identical or different, represents a group selected from the group consisting of an alkyl and hydroxyalkyl group having 1 to 4 carbon atoms and X represents an inorganic or organic ion.
Claim 5
Dependent Claim
23 words
The method of claim 1, in which the cation-exchanging groups are selected from the group consisting of carboxylic, sulphonic and phosphonic acid functions.
Claim 6
Dependent Claim
91 words
The method of claim 1, in which the cross-linked polymer is derived from monomers selected from the group consisting of (a) epoxy compounds cross-linked with polyamines as catalyst, (b) formaldehyde cross-linked by polycondensation with monomers selected from the group consisting of urea, melamine, polyamines and phenols, (c) vinyl monomers selected from the group consisting of vinyl pyridine, styrene and derivatives thereof, vinylbenzoic acid, acrylic acid and methacrylic acid cross-linked with polyfunctional monomers selected from the group consisting of mono- or polyalkyleneglycol diacrylate or dimethacrylate, divinylbenzene, vinyltrialkoxysilane, vinyltrihalogenosilane and bis methylene acrylamide.
Claim 7
Dependent Claim
36 words
The method of claim 1, in which the proteins are selected from the group consisting of albumin, lactalbumins, egg albumin, serum albumin, haemoglobin, α, β and γ-globulins, lactoglobulins, fibrinogen, urease, trypsin, lysozyme, pepsin, proteases and cytochrome.
Claim 8
Dependent Claim
30 words
The method of claim 1, in which the protein solutions to be treated are selected from the group consisting of milk serum, beer, blood, extracts from organs and industrial effluents.
Claim 9
Dependent Claim
28 words
The method of claim 1, in which the required protein remains in the solution, and the other proteins in the solution are fixed on the ion exchange resin.
Claim 10
Dependent Claim
16 words
The method of claim 1, in which all the proteins are fixed and the solution purified.
Claim 11
Dependent Claim
20 words
The method as claimed in claim 1 in which the exchanger has an exchange capacity of less than 2 meg./g.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-07-10 | 1982-01-09 | 1982-07-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-07-10 | 1986-01-09 | 1986-07-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-07-10 | 1990-01-09 | 1990-07-11 | $7700.00 | Window Closed / Lapsed |