Patent US4981798A successfully refreshed from Google Patents.
US4981798A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immobilization of growing microorganisms on polymer-coated cotton gauze
Assignee: Japan Atomic Energy Agency
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Filing: 1989-08-01
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Granted: 1991-01-01
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Expires: 2009-08-01
Abstract
Abstract A carrier for immobilization of microorganisms is prepared by impregnating a fabric such as cotton gauze having a mesh size of 10-50 with 20-50% by weight of a vinyl monomer such as ethlene glycol methacrylate and polymerizing the monomer with ionizing radiation to form a polymer coating on the fabric. A microorganism such as Trichoderma reesei is grown in a liquid culture medium in contact with the polymer-coated fabric and the polymer-coated fabric is recovered containing growing cells of the microorganism. The polymer-coated fabric has high air permeability and permits good diffusion of liquid culture medium.
Inventors
- 👤 Minoru Kamakura
- 👤 Masao Tamada
- 👤 Noboru Kasai
- 👤 Isao Kaetsu
- 👤 Shigeru Yamanaka
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 13:44
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/10; C12N11/12; D; D06; D06M; D06M16/00; D06M16/003; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/945
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
109 words
A process for preparing a microorganism immobilized on a carrier made of a polymer-coated cotton gauze comprising the following steps: (a) impregnating a sheet of cotton gauze having a mesh size of 10-50 with 20-50% weight of ethylene glycol methacrylate; (b) irradiating the impregnated gauze with ionizing radiation thereby to polymerize said ethylene glycol methacrylate to obtain a two-dimensional carrier made of a polymer-coated cotton gauze; (c) contacting said carrier with a liquid culture medium in which Trichoderma reesei cells can grow; (d) inoculating said medium with said cells to culture the cells on said polymer-coated cotton gauze; and (e) recovering a polymer-coated cotton gauze comprising the growing cells.
Claim 2
Dependent Claim
109 words
A process for preparing a microorganism immobilized on a carrier made of a polymer-coated cotton gauze comprising the following steps: (a) impregnating a sheet of cotton gauze having a mesh size of 10-50 with 20-50% weight of ethylene glycol methacrylate; (b) irradiating the impregnated gauze with ionizing radiation thereby to polymerize said ethylene glycol methacrylate to obtain a two-dimensional carrier made of a polymer-coated cotton gauze; (c) contacting said carrier with a liquid culture medium in which Trichoderma reesei cells can grow; (d) inoculating said medium with said cells to culture the cells on said polymer-coated cotton gauze; and (e) recovering a polymer-coated cotton gauze comprising the growing cells.
Claim 3
Dependent Claim
59 words
The process according to claim 1 wherein the ionizing radiation is selected from the group consisting of gamma rays and X rays, and the radiation is applied at a dosage rate of from 1×10 4 to 1×10 6 r/hr to give a total dosage of from 0.5 to 1 Mrad at a temperature ranging from 0 to 100° C.
Claim 4
Dependent Claim
16 words
The process according to claim 1 wherein the temperature range is from ambient to 100° C.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-12-31 | 1994-07-02 | 1995-01-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-12-31 | 1998-07-02 | 1999-01-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-12-31 | 2002-07-02 | 2003-01-01 | $7700.00 | Window Closed / Lapsed |