US4338401A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immobilization of enzymes
Assignee: Italfarmaco SpA
•
Filing: 1980-08-04
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Granted: 1982-07-06
•
Expires: 2000-08-04
Abstract
Abstract Enzyme immobilization is carried out be adding to a suspension of polysaccharide in aqueous medium, a vinyl monomer and an enzyme, then a catalyst comprising a metallic salt, and irradiating the resulting mixture with ultraviolet light to cause polymerization and form a copolymer of the polysaccharide having enzymatic activity. The metallic salt is preferably a ferric salt and the vinyl monomer is preferably methylacrylate, glycidylmethacrylate, acrylonitride, bis-acyloylpiperazine or symmetrical N, N', N"-trisacryloyl-hexahydrotriazine.
Inventors
- 👤 Pietro Cremonesi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 11:41
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/06; C12N11/10; C12N11/12
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
103 words
A process for the preparation of a copolymer of a polysaccharide having enzymatic activity, which comprises adding to a suspension of a polysaccharide in an aqueous medium, a vinyl monomer and an enzyme, wherein the vinyl monomer is an N-acrloyl derivative of a nitrogenous heterocyclic compound or a monomer of the formula ##STR2## in which R is hydrogen or methyl, R' is CN or COOR", and R" is lower alkyl, epoxy substituted lower alkyl or hydroxy substituted lower alkyl, then adding a catalyst which comprises a ferric salt and irradiating the resulting mixture with ultraviolet light to cause polymerization and form said copolymer.
Claim 2
Dependent Claim
103 words
A process for the preparation of a copolymer of a polysaccharide having enzymatic activity, which comprises adding to a suspension of a polysaccharide in an aqueous medium, a vinyl monomer and an enzyme, wherein the vinyl monomer is an N-acrloyl derivative of a nitrogenous heterocyclic compound or a monomer of the formula ##STR2## in which R is hydrogen or methyl, R' is CN or COOR", and R" is lower alkyl, epoxy substituted lower alkyl or hydroxy substituted lower alkyl, then adding a catalyst which comprises a ferric salt and irradiating the resulting mixture with ultraviolet light to cause polymerization and form said copolymer.
Claim 3
Dependent Claim
22 words
The process according to claim 1 wherein in said vinyl monomer R" is epoxy substituted lower alkyl or hydroxy substituted lower alkyl.
Claim 4
Dependent Claim
12 words
The process according to claim 1 wherein said vinyl monomer is methylacrylate.
Claim 5
Dependent Claim
12 words
The process according to claim 1 wherein the vinyl monomer is glycidylmethacrylate.
Claim 6
Dependent Claim
12 words
The process according to claim 1 wherein the vinyl monomer is acrylonitrile.
Claim 7
Dependent Claim
22 words
The process according to claim 1 wherein said vinyl monomer is a N-acryloyl derivative of a compound containing a nitrogenous heterocyclic ring.
Claim 8
Dependent Claim
12 words
The process according to claim 6 wherein the vinyl monomer is bis-acryloylpiperazine.
Claim 9
Dependent Claim
13 words
The process according to claim 6 wherein the vinyl monomer is symmetrical N,N',N"-trisacryloyl-hexahydrotriazine.
Claim 10
Dependent Claim
23 words
The process according to claim 1 wherein the enzyme is a proteolytic enzyme selected from the group consisting of papain, trypsin and chymotrypsin.
Claim 11
Dependent Claim
31 words
The process according to claim 1 wherein the enzyme is a hydrolytic enzyme which is a member selected from the group consisting of amylases, cellulases, β-glucuronidase-arylsulfatases, esterases, heparinases, penicillinamydases and ureases.
Claim 12
Dependent Claim
27 words
The process according to claim 1 wherein the enzyme is an oxidase which is a member selected from the group consisting of aminoacidoxidases, diaphorases, glucoseoxidases and peroxidases.
Claim 13
Dependent Claim
24 words
The process according to claim 1 wherein the enzyme is a dehydrogenase selected from the group consisting of steroidodehydrogenase lactic acid hydrogenase and alcohol-dehydrogenase.
Claim 14
Dependent Claim
12 words
The process according to claim 1 wherein the enzyme is a catalase.
Claim 15
Dependent Claim
25 words
The process according to claim 1 wherein the enzyme is an isomerase which is a member selected from the group consisting of invertases and steroidoisomerases.
References Cited (Backward Citations)
US3860490A
Cited
US3925157A
Cited
US3957580A
Cited
US3985616A
Cited
US4194066A
Cited
US4226938A
Cited
US4195129A
Cited
US4177107A
Cited
US4272617A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-07-05 | 1986-01-04 | 1986-07-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-07-05 | 1990-01-04 | 1990-07-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-07-05 | 1994-01-04 | 1994-07-06 | $7700.00 | Window Closed / Lapsed |