Patent US4338401A successfully refreshed from Google Patents.
US4338401A
Expired / Lapsed
US
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Immobilization of enzymes
Assignee: Italfarmaco SpA
•
Filing: 1980-08-04
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Granted: 1982-07-06
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Expires: 2000-08-04
Abstract
Abstract Enzyme immobilization is carried out be adding to a suspension of polysaccharide in aqueous medium, a vinyl monomer and an enzyme, then a catalyst comprising a metallic salt, and irradiating the resulting mixture with ultraviolet light to cause polymerization and form a copolymer of the polysaccharide having enzymatic activity. The metallic salt is preferably a ferric salt and the vinyl monomer is preferably methylacrylate, glycidylmethacrylate, acrylonitride, bis-acyloylpiperazine or symmetrical N, N', N"-trisacryloyl-hexahydrotriazine.
Inventors
- 👤 Pietro Cremonesi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 12:18
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/06; C12N11/10; C12N11/12
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
103 words
A process for the preparation of a copolymer of a polysaccharide having enzymatic activity, which comprises adding to a suspension of a polysaccharide in an aqueous medium, a vinyl monomer and an enzyme, wherein the vinyl monomer is an N-acrloyl derivative of a nitrogenous heterocyclic compound or a monomer of the formula ##STR2## in which R is hydrogen or methyl, R' is CN or COOR", and R" is lower alkyl, epoxy substituted lower alkyl or hydroxy substituted lower alkyl, then adding a catalyst which comprises a ferric salt and irradiating the resulting mixture with ultraviolet light to cause polymerization and form said copolymer.
Claim 2
Dependent Claim
103 words
A process for the preparation of a copolymer of a polysaccharide having enzymatic activity, which comprises adding to a suspension of a polysaccharide in an aqueous medium, a vinyl monomer and an enzyme, wherein the vinyl monomer is an N-acrloyl derivative of a nitrogenous heterocyclic compound or a monomer of the formula ##STR2## in which R is hydrogen or methyl, R' is CN or COOR", and R" is lower alkyl, epoxy substituted lower alkyl or hydroxy substituted lower alkyl, then adding a catalyst which comprises a ferric salt and irradiating the resulting mixture with ultraviolet light to cause polymerization and form said copolymer.
Claim 3
Dependent Claim
22 words
The process according to claim 1 wherein in said vinyl monomer R" is epoxy substituted lower alkyl or hydroxy substituted lower alkyl.
Claim 4
Dependent Claim
12 words
The process according to claim 1 wherein said vinyl monomer is methylacrylate.
Claim 5
Dependent Claim
12 words
The process according to claim 1 wherein the vinyl monomer is glycidylmethacrylate.
Claim 6
Dependent Claim
12 words
The process according to claim 1 wherein the vinyl monomer is acrylonitrile.
Claim 7
Dependent Claim
22 words
The process according to claim 1 wherein said vinyl monomer is a N-acryloyl derivative of a compound containing a nitrogenous heterocyclic ring.
Claim 8
Dependent Claim
12 words
The process according to claim 6 wherein the vinyl monomer is bis-acryloylpiperazine.
Claim 9
Dependent Claim
13 words
The process according to claim 6 wherein the vinyl monomer is symmetrical N,N',N"-trisacryloyl-hexahydrotriazine.
Claim 10
Dependent Claim
23 words
The process according to claim 1 wherein the enzyme is a proteolytic enzyme selected from the group consisting of papain, trypsin and chymotrypsin.
Claim 11
Dependent Claim
31 words
The process according to claim 1 wherein the enzyme is a hydrolytic enzyme which is a member selected from the group consisting of amylases, cellulases, β-glucuronidase-arylsulfatases, esterases, heparinases, penicillinamydases and ureases.
Claim 12
Dependent Claim
27 words
The process according to claim 1 wherein the enzyme is an oxidase which is a member selected from the group consisting of aminoacidoxidases, diaphorases, glucoseoxidases and peroxidases.
Claim 13
Dependent Claim
24 words
The process according to claim 1 wherein the enzyme is a dehydrogenase selected from the group consisting of steroidodehydrogenase lactic acid hydrogenase and alcohol-dehydrogenase.
Claim 14
Dependent Claim
12 words
The process according to claim 1 wherein the enzyme is a catalase.
Claim 15
Dependent Claim
25 words
The process according to claim 1 wherein the enzyme is an isomerase which is a member selected from the group consisting of invertases and steroidoisomerases.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-07-05 | 1986-01-04 | 1986-07-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-07-05 | 1990-01-04 | 1990-07-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-07-05 | 1994-01-04 | 1994-07-06 | $7700.00 | Window Closed / Lapsed |