US4194066A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immobilization of enzymes or bacteria cells
Assignee: Japan Atomic Energy Agency
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Filing: 1975-08-20
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Granted: 1980-03-18
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Expires: 1995-08-20
Abstract
Abstract A porous polymer containing immobilized enzymes or bacteria cells is prepared by forming a mixture of enzymes or bacteria cells and a monomer having the formulae <IMAGE> <IMAGE> wherein X is hydrogen or methyl; R is -CH2CH2-, <IMAGE> or -CH2CH2CH2-, R' is alkyl or hydrogen; n is integer of 2 or more and m is integer of 4 or more, or mixture of such monomers; and then polymerizing or copolymerizing the monomer(s) in the mixture at a temperature of less than 0 DEG C. by means of ionizing radiation.
Inventors
- 👤 Isao Kaetsu
- 👤 Minoru Kumkaura
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 03:46
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/04; C12N11/08; C12N11/082; C12N11/087
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
124 words
A process for producing a porous polymer composition containing immobilized enzymes and/or immobilized bacterial cells which comprises: forming a mixture consisting essentially of an enzyme solution or a dispersion of bacterial cells and a monomer having the formula ##STR11## wherein X is hydrogen or methyl, R is --CH 2 CH 2 --, ##STR12## or --CH 2 CH 2 CH 2 --, R' is hydrogen or alkyl, n is an integer of 2 or more and m is an integer of 4 or more, or a mixture of such monomers; and then polymerizing and/or copolymerizing the monomer(s) containied in the mixture at a temperature of less than 0° C. by means of an ionizing radiation, thereby depositing the polymer containing enzyme and/or cells from water.
Claim 2
Dependent Claim
124 words
A process for producing a porous polymer composition containing immobilized enzymes and/or immobilized bacterial cells which comprises: forming a mixture consisting essentially of an enzyme solution or a dispersion of bacterial cells and a monomer having the formula ##STR11## wherein X is hydrogen or methyl, R is --CH 2 CH 2 --, ##STR12## or --CH 2 CH 2 CH 2 --, R' is hydrogen or alkyl, n is an integer of 2 or more and m is an integer of 4 or more, or a mixture of such monomers; and then polymerizing and/or copolymerizing the monomer(s) containied in the mixture at a temperature of less than 0° C. by means of an ionizing radiation, thereby depositing the polymer containing enzyme and/or cells from water.
Claim 3
Dependent Claim
147 words
A process for producing a porous polymer composition containing immobilized enzyme and/or immobilized bacterial cells which comprises: mixing (a) an enzyme solution or a dispersion of bacterial cells, (b) a monomer having the formula ##STR13## wherein X is hydrogen or methyl, R is --CH 2 CH 2 --, ##STR14## or --CH 2 CH 2 CH 2 --, R' is hydrogen or alkyl, n is an integer of 2 or more and m is an integer of 4 or more, or a mixture of such monomers, and (c) one or more monomers other than said (b) monomers in the amount of less than 40% by weight on the basis of the total monomers; and then polymerizing and/or copolymerizing the monomers contained in the mixture, at a temperature of less than 0° C. by means of an ionizing radiation, thereby depositing the polymer containing enzyme and/or cells from water.
Claim 4
Dependent Claim
147 words
A process for producing a porous polymer composition containing immobilized enzyme and/or immobilized bacterial cells which comprises: mixing (a) an enzyme solution or a dispersion of bacterial cells, (b) a monomer having the formula ##STR13## wherein X is hydrogen or methyl, R is --CH 2 CH 2 --, ##STR14## or --CH 2 CH 2 CH 2 --, R' is hydrogen or alkyl, n is an integer of 2 or more and m is an integer of 4 or more, or a mixture of such monomers, and (c) one or more monomers other than said (b) monomers in the amount of less than 40% by weight on the basis of the total monomers; and then polymerizing and/or copolymerizing the monomers contained in the mixture, at a temperature of less than 0° C. by means of an ionizing radiation, thereby depositing the polymer containing enzyme and/or cells from water.
Claim 5
Dependent Claim
33 words
The porous polymer composition prepared by the process of claim 1, containing immobilized enzyme and/or immobilized bacterial cells, characterized in that in said composition the enzyme or cells are dispersed within the polymer.
Claim 6
Dependent Claim
33 words
The porous polymer composition prepared by the process of claim 2, containing immobilized enzyme and/or immobilized bacterial cells, characterized in that in said compositions the enzyme or cells are dispersed within the polymer.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-03-18 | 1983-09-17 | 1984-03-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-03-18 | 1987-09-17 | 1988-03-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-03-18 | 1991-09-17 | 1992-03-18 | $7700.00 | Window Closed / Lapsed |