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US4487831A
Expired / Lapsed
US
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Process for the conversion of cellulose to glucose
Assignee: RESEARCH CORPORATON A NEW YORK NOT FOR PROFIT CORP
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Filing: 1982-05-19
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Granted: 1984-12-11
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Expires: 2002-05-19
Abstract
Abstract Cellulose is converted to glucose in a two stage process in which cellobiose is produced from a cellulosic feedstock under the influence of Trichoderma reesei in a first stage and cellobiose from the first stage is converted to glucose in a second stage by the action of purified cellobiase derived from Aspergillus terreus. Cellobiase from A. terreus is purified by contacting a crude aqueous extract of the cellobiase with an ion exchange resin and an anion exchange resin. The purified cellobiase may be immobilized on a suitable substrate.
Inventors
- 👤 Donal F. Day
- 👤 Wesley E. Workman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 04:59
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/14; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/2445; C12Y; C12Y302/00; C12Y302/01; C12Y302/01021; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815; Y10S435/8215; Y10S435/911; Y10S435/913; Y10S435/945
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
75 words
A method for converting cellobiose to glucose which comprises contacting cellobiose with a purified cellobiase enzyme prepared by extracting a biomass of a cellobiase-producing mold with an aqueous buffer thereby producing a crude enzyme extract, contacting said crude enzyme extract at a pH of 6 with anion and cation exchange resins effecting removal of a major portion of the contaminating protein associated therewith, and contacting the purified cellobiase enzyme with cellobiose under cellobiose conversion conditions.
Claim 2
Independent Claim
75 words
A method for converting cellobiose to glucose which comprises contacting cellobiose with a purified cellobiase enzyme prepared by extracting a biomass of a cellobiase-producing mold with an aqueous buffer thereby producing a crude enzyme extract, contacting said crude enzyme extract at a pH of 6 with anion and cation exchange resins effecting removal of a major portion of the contaminating protein associated therewith, and contacting the purified cellobiase enzyme with cellobiose under cellobiose conversion conditions.
Claim 3
Dependent Claim
15 words
A method according to claim 1 wherein said cellobiase enzyme is extracted from Aspergillus terreus.
Claim 4
Dependent Claim
22 words
A method according to claim 2 wherein said anion exchange resin contains diethylaminoethyl groups and said cation exchange resin contains carboxymethyl groups.
Claim 5
Dependent Claim
19 words
A method according to claim 1 wherein said aqueous buffer comprises a 0.05 molar aqueous solution of potassium phosphate.
Claim 6
Dependent Claim
21 words
A method according to claim 1 wherein said cellobiose is derived from a cellulosic feedstock by the action of Trichoderma reesei.
Claim 7
Independent Claim
45 words
A method for purifying an aqueous cellobiase enzyme extract prepared from a cellobiase-producing mold which comprises contacting said extract at a pH of 6 with anion and cation exchange resins thereby effecting removal of a major portion of the contaminating protein associated with said enzyme.
Claim 8
Independent Claim
45 words
A method for purifying an aqueous cellobiase enzyme extract prepared from a cellobiase-producing mold which comprises contacting said extract at a pH of 6 with anion and cation exchange resins thereby effecting removal of a major portion of the contaminating protein associated with said enzyme.
Claim 9
Dependent Claim
22 words
A method according to claim 6 wherein said anion exchange resin comprises diethylaminoethyl groups and said cation exchange resin comprises carboxymethyl groups.
Claim 10
Dependent Claim
16 words
A method according to claim 6 wherein said cellobiase enzyme is extracted from Aspergillus terreus cells.
Claim 11
Dependent Claim
106 words
A process for converting cellulose to glucose comprising, contacting a cellulosic feedstock with a biomass containing Trichoderma reesei in an aqueous medium under cellulose conversion conditions to produce cellobiose, separating the aqueous solution containing cellobiose from said biomass, and contacting under cellobiose conversion conditions said aqueous solution containing the cellobiose with a purified cellobiase anzyme prepared by extracting a biomass of a cellobiase-producing mold with an aqueous buffer to produce a crude enzyme extract and contacting said crude enzyme extract at a pH of 6 with anion and cation exchange resins thereby effecting removal of a major portion of the contaminating protein associated with said enzyme.
Claim 12
Dependent Claim
106 words
A process for converting cellulose to glucose comprising, contacting a cellulosic feedstock with a biomass containing Trichoderma reesei in an aqueous medium under cellulose conversion conditions to produce cellobiose, separating the aqueous solution containing cellobiose from said biomass, and contacting under cellobiose conversion conditions said aqueous solution containing the cellobiose with a purified cellobiase anzyme prepared by extracting a biomass of a cellobiase-producing mold with an aqueous buffer to produce a crude enzyme extract and contacting said crude enzyme extract at a pH of 6 with anion and cation exchange resins thereby effecting removal of a major portion of the contaminating protein associated with said enzyme.
Claim 13
Dependent Claim
46 words
An enzyme composition for converting cellulose to glucose comprising purified cellobiase enzyme extracted from Aspergillus terreus characterized as having a Ki dissociation constant of 3.5×10 -3 M for glucose and a Km affinity of 4.0×10 -4 M for cellulose and cellulase enzymes extracted from Trichoderma reesei.
Claim 14
Dependent Claim
46 words
An enzyme composition for converting cellulose to glucose comprising purified cellobiase enzyme extracted from Aspergillus terreus characterized as having a Ki dissociation constant of 3.5×10 -3 M for glucose and a Km affinity of 4.0×10 -4 M for cellulose and cellulase enzymes extracted from Trichoderma reesei.
Claim 15
Dependent Claim
22 words
An immobilized enzyme composition for the conversion of cellulose to glucose, comprising the composition of claim 10 and a suitable carrier material.
Claim 16
Dependent Claim
16 words
A method for converting cellulose to glucose comprising contacting cellulose with the composition of claim 10.
Claim 17
Dependent Claim
17 words
A method according to claim 6 wherein said purified cellobiase enzyme is immobilized on a carrier material.
Claim 18
Dependent Claim
13 words
A process of converting cellobiose to glucose employing the method of claim 6.
Claim 19
Dependent Claim
14 words
The process of claim 9 wherein the cellobiase enzyme is extracted from Aspergillus terreus.
Claim 20
Dependent Claim
32 words
A purified enzyme comprising cellobiase extracted from Aspergillus terreus characterized as having a Ki dissociation constant of 3.5×10 -3 M for glucose and a Km affinity of 4×10 -4 M for cellulose.
Claim 21
Dependent Claim
32 words
A purified enzyme comprising cellobiase extracted from Aspergillus terreus characterized as having a Ki dissociation constant of 3.5×10 -3 M for glucose and a Km affinity of 4×10 -4 M for cellulose.
Claim 22
Dependent Claim
14 words
The enzyme of claim 16 wherein said enzyme is immobilized on a carrier material.
Claim 23
Independent Claim
42 words
A method of converting cellobiose to glucose comprising contacting cellobiose with a purified cellobiase enzyme extracted from Aspergillus terreus characterized as having a Ki dissociation constant of 3.5×10 -3 M for glucose and a Km affinity of 4.0×10 -4 M for cellobiose.
Claim 24
Independent Claim
42 words
A method of converting cellobiose to glucose comprising contacting cellobiose with a purified cellobiase enzyme extracted from Aspergillus terreus characterized as having a Ki dissociation constant of 3.5×10 -3 M for glucose and a Km affinity of 4.0×10 -4 M for cellobiose.
Claim 25
Dependent Claim
16 words
A method according to claim 18 wherein the purified cellobiase is immobilized as a carrier material.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-12-11 | 1988-06-11 | 1988-12-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-12-11 | 1992-06-11 | 1992-12-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-12-11 | 1996-06-11 | 1996-12-11 | $7700.00 | Window Closed / Lapsed |