Patent US4965204A successfully refreshed from Google Patents.
US4965204A
Expired / Lapsed
US
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Human stem cells and monoclonal antibodies
Assignee: Johns Hopkins University
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Filing: 1987-06-01
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Granted: 1990-10-23
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Expires: 2007-06-01
Abstract
Abstract Monoclonal antibodies that recognize a stage-specific antigen on immature human marrow cells are provided. These antibodies are useful in methods of isolating cell suspensions from human blood and marrow that can be employed in bone marrow transplantation. Cell suspensions containing human pluripotent lympho-hematopoietic stem cells are also provided, as well as therapeutic methods employing the cell suspensions.
Inventors
- 👤 Curt I. Civin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 12:44
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; Y; Y10; Y10S; Y10S530/00; Y10S530/808; Y10S530/809
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
129 words
A monoclonal antibody which specifically binds to an antigen on non-malignant, immature human marrow cells, wherein said antigen is stage specific and not lineage dependent, and said antigen is also specifically bound by the antibody produced by the hybridoma deposited under ATCC Accession No. HB-8483; (a) which antigen is present on non-malignant, human blood or bone marrow: (i) colony-forming cells for granulocytes and monocytes (CFC-GM), (ii) colony-forming cells for erythrocytes (BFU-E), (iii) colony-forming cells for eosinophils (CFC-Eo), (iv) multipotent colony-forming cells (CFC-GEMM), and (v) immature lymphoid precursor cells; (b) which antigen is present on a maximum of about 5% non-malignant, human marrow cells and a maximum of about 1% non-malignant, human peripheral blood cells; and (c) which antigen is not present on non-malignant, mature human myeloid and lymphoid cells.
Claim 2
Dependent Claim
129 words
A monoclonal antibody which specifically binds to an antigen on non-malignant, immature human marrow cells, wherein said antigen is stage specific and not lineage dependent, and said antigen is also specifically bound by the antibody produced by the hybridoma deposited under ATCC Accession No. HB-8483; (a) which antigen is present on non-malignant, human blood or bone marrow: (i) colony-forming cells for granulocytes and monocytes (CFC-GM), (ii) colony-forming cells for erythrocytes (BFU-E), (iii) colony-forming cells for eosinophils (CFC-Eo), (iv) multipotent colony-forming cells (CFC-GEMM), and (v) immature lymphoid precursor cells; (b) which antigen is present on a maximum of about 5% non-malignant, human marrow cells and a maximum of about 1% non-malignant, human peripheral blood cells; and (c) which antigen is not present on non-malignant, mature human myeloid and lymphoid cells.
Claim 3
Dependent Claim
22 words
The monoclonal antibody of claim 1 that corresponds to the monoclonal antibody produced by the hybridoma deposited under ATCC Accession No. HB-8483.
Claim 4
Dependent Claim
20 words
The monoclonal antibody of claim 1 that is the antibody produced by the hybridoma deposited under ATCC Accession No. HB-8483.
Claim 5
Dependent Claim
10 words
A hydbridoma which produces a monoclonal antibody of claim 1.
Claim 6
Dependent Claim
10 words
A hybridoma which produces a monoclonal antibody of claim 2.
Claim 7
Dependent Claim
8 words
The hybridoma deposited under ATCC Accession No. HB-8483.
Claim 8
Dependent Claim
8 words
The hybridoma deposited under ATCC Accession No. HB-8483.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-10-22 | 1994-04-23 | 1994-10-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-10-22 | 1998-04-23 | 1998-10-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-10-22 | 2002-04-23 | 2002-10-23 | $7700.00 | Window Closed / Lapsed |