Patent US4956289A successfully refreshed from Google Patents.
US4956289A
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Thin film membrane enzyme reactor and method of using same
Assignee: Brunswick Corp
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Filing: 1987-03-16
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Granted: 1990-09-11
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Expires: 2007-03-16
Abstract
Abstract An enzyme reactor system is provided based on the entrapment of an enzyme in a hydrogel layer coated on a support, and confined by an ultraporous thin film membrane diffusion barrier. The diffusion barrier confines the enzyme, but lets substrate and reaction products diffuse feeely into and out of the hydrogel layer. In an alternate embodiment, the support is formed of an ultraporous thin film membrane diffusion barrier on a microporous or macroporous support, through which the reaction products can diffuse freely, but through which neither enzyme nor substrate can pass. In this embodiment, the product is recovered in high purity, free of substrate and enzyme.
Inventors
- 👤 Wolfgang J. Wrasidlo
- 👤 Frieder K. Hofmann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 22:08
CPC Classifications
C; C12; C12M; C12M25/00; C12M25/14; C12M21/00; C12M21/18; C12M29/00; C12M29/04; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
101 words
An enzyme reactor consisting essentially of: a support; an enzyme hydrogel layer physically entrapped on said support; and a porous thin film diffusion membrane on said enzyme hydrogel layer, wherein said thin film diffusion membrane has a thickness of from about 0.0012 to 0.2 microns and a pore size sufficient to permit diffusion of a substrate for said enzyme and of the product of a reaction of said substrate catalyzed by said enzyme, but which will not permit said enzyme to pass through said thin film diffusion membrane, said thin film diffusion membrane adapted to direct exposure of a process stream.
Claim 2
Dependent Claim
101 words
An enzyme reactor consisting essentially of: a support; an enzyme hydrogel layer physically entrapped on said support; and a porous thin film diffusion membrane on said enzyme hydrogel layer, wherein said thin film diffusion membrane has a thickness of from about 0.0012 to 0.2 microns and a pore size sufficient to permit diffusion of a substrate for said enzyme and of the product of a reaction of said substrate catalyzed by said enzyme, but which will not permit said enzyme to pass through said thin film diffusion membrane, said thin film diffusion membrane adapted to direct exposure of a process stream.
Claim 3
Dependent Claim
10 words
The reactor of claim 1, wherein said support is impermeable.
Claim 4
Dependent Claim
36 words
The reactor of claim 1, wherein said support comprises: a support surface thin film layer in contact with said enzyme which is impermeable, and an open porous layer physically supporting said support surface thin film layer.
Claim 5
Dependent Claim
62 words
The reactor of claim 1, wherein said support comprises: a support surface thin film layer in contact with said enzyme which is porous to the product of a reaction of a substrate and said enzyme, but which will not permit said enzyme to pass through said support surface layer, and an open porous layer physically supporting said support surface thin film layer.
Claim 6
Dependent Claim
35 words
The reactor of claim 4, wherein there is interposed between said support surface thin film layer and said open porous layer physically supporting said support surface thin film layer a thin, freely porous gel layer.
Claim 7
Dependent Claim
65 words
The reactor of claim 1, wherein said support comprises: a support surface thin film layer in contact with said enzyme which is porous to the product of a reaction of a substrate and said enzyme, but which will not permit said substrate or said enzyme to pass through said support surface layer, and an open porous layer physically supporting said support surface thin film layer.
Claim 8
Dependent Claim
35 words
The reactor of claim 6, wherein there is interposed between said support surface thin film layer and said open porous layer physically supporting said support surface thin film layer a thin, freely porous gel layer.
Claim 9
Dependent Claim
20 words
The reactor of claim 1, wherein said support is a dense support having a high surface area per unit volume.
Claim 10
Dependent Claim
21 words
The reactor of claim 1, wherein said enzyme hydrogel comprises an aqueous gel of an enzyme and a hydrogel forming polymer.
Claim 11
Dependent Claim
13 words
The reactor of claim 9, wherein said hydrogel forming polymer is cross linked.
Claim 12
Dependent Claim
22 words
The reactor of claim 9, wherein said hydrogel forming polymer is irreversibly bound to said support and to said thin film membrane.
Claim 13
Dependent Claim
37 words
The reactor of claim 9, wherein said enzyme is physically entrapped in said hydrogel forming polymer in an amount of from about 10 to about 90 weight percent of said hydrogel forming polymer, on a solids basis.
Claim 14
Independent Claim
171 words
A method for using an enzyme reactor the steps consisting essentially of: forming an enzyme reactor which comprises: a support; an enzyme hydrogel layer physically entrapped on said support; and a porous thin film diffusion membrane on said enzyme hydrogel layer, wherein said thin film diffusion membrane has a thickness of from about 0.0012 to 0.2 microns and a pore size sufficient to permit diffusion of a substrate for said enzyme and of the product of a reaction of said substrate catalyzed by said enzyme, but which will not permit said enzyme to pass through said thin film diffusion membrane; placing said enzyme reactor into a reactor vessel; dissolving substrate for said enzyme in a solvent therefor; passing said dissolved substrate through said reactor vessel in such a manner that said substrate and said solvent therefor are in direct exposure to said porous thin film diffusion membrane; allowing said substrate to interact with said enzyme, thereby forming a product; collecting the product of said interaction of said substrate and said enzyme.
Claim 15
Independent Claim
171 words
A method for using an enzyme reactor the steps consisting essentially of: forming an enzyme reactor which comprises: a support; an enzyme hydrogel layer physically entrapped on said support; and a porous thin film diffusion membrane on said enzyme hydrogel layer, wherein said thin film diffusion membrane has a thickness of from about 0.0012 to 0.2 microns and a pore size sufficient to permit diffusion of a substrate for said enzyme and of the product of a reaction of said substrate catalyzed by said enzyme, but which will not permit said enzyme to pass through said thin film diffusion membrane; placing said enzyme reactor into a reactor vessel; dissolving substrate for said enzyme in a solvent therefor; passing said dissolved substrate through said reactor vessel in such a manner that said substrate and said solvent therefor are in direct exposure to said porous thin film diffusion membrane; allowing said substrate to interact with said enzyme, thereby forming a product; collecting the product of said interaction of said substrate and said enzyme.
Claim 16
Dependent Claim
27 words
The method of claim 13, wherein said substrate diffuses through said thin film diffusion membrane and into said enzyme hydrogel layer prior to interacting with said enzyme.
Claim 17
Dependent Claim
34 words
The method of claim 13, wherein said product is collected by allowing said product to diffuse from said enzyme hydrogel layer through said thin film diffusion membrane and into said solvent for said substrate.
Claim 18
Dependent Claim
66 words
The method of claim 13, wherein said support further comprises a support surface thin film layer in contact with said enzyme which is porous to the product of a reaction of a substrate and said enzyme, but which will not permit said subsrate or said enzyme to pass through said support surface layer, and an open porous layer physically supporting said support surface thin film layer.
Claim 19
Dependent Claim
27 words
The method of claim 16, wherein said substrate diffuses through said thin film diffusion membrane and into said enzyme hydrogel layer prior to interacting with said enzyme.
Claim 20
Dependent Claim
38 words
The method of claim 16, wherein there is a solvent for said product in contact with the surface of said open porous layer of said support which is not in contact with said support surface thin film layer.
Claim 21
Dependent Claim
30 words
The method of claim 18, wherein said product diffuses through said support surface thin film layer and said open porous layer of said support into said solvent for said product.
Claim 22
Dependent Claim
17 words
The method of claim 18, wherein said said substrate is soluble in said solvent for said product.
Claim 23
Dependent Claim
16 words
The method of claim 18, wherein said substrate is insoluble in said solvent for said product.
Claim 24
Dependent Claim
16 words
The method of claim 18, wherein said product is insoluble in said solvent for said substrate.
Claim 25
Dependent Claim
35 words
The method of claim 16, wherein there is interposed between said support surface thin film layer and said open porous layer physically supporting said support surface thin film layer a thin, freely porous gel layer.
Claim 26
Dependent Claim
38 words
The method of claim 23, wherein there is a solvent for said product in contact with the surface of said open porous layer of said support which is not in contact with said support surface thin film layer.
Claim 27
Dependent Claim
30 words
The method of claim 23, wherein said product diffuses through said support surface thin film layer and said open porous layer of said support into said solvent for said product.
Claim 28
Dependent Claim
17 words
The method of claim 23, wherein said said substrate is soluble in said solvent for said product.
Claim 29
Dependent Claim
16 words
The method of claim 23, wherein said substrate is insoluble in said solvent for said product.
Claim 30
Dependent Claim
16 words
The method of claim 23, wherein said product is insoluble in said solvent for said substrate.
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