US4952812A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Irradiation of blood products
Assignee: Fenwal Inc
•
Filing: 1989-05-02
•
Granted: 1990-08-28
•
Expires: 2009-05-02
Abstract
Abstract A thin film or layer of white blood cells, such as the contaminating white cells in a platelet concentrate, is irradiated with ultraviolet radiation predominately of a wavelength of 280 to 320 nanometers, and preferably at an intensity of 4 to 20 milliwatts per square cm. to provide a total energy exposure of typically 800 to 20,000 millijoules per square cm. of ultraviolet radiation. The white blood cells so produced substantially lose their capability to set off an immune reaction in an alloimmunized patient. The white blood cells may preferably be placed into such film in a flat, flexible bag made of poly(ethylene-vinyl acetate) plastic, with the flat, flexible bag being stretched in a direction normal to the path of ultraviolet radiation. Novel irradiating devices are also disclosed.
Inventors
- 👤 Jeffrey E. Miripol
- 👤 Arnold Bilstad
- 👤 John Foley
- 👤 Dean Glash
- 👤 William R. Bratten
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 18:53
CPC Classifications
A; A61; A61M; A61M1/00; A61M1/36; A61M1/3681; A61L; A61L2/00; A61L2/02; A61L2/08; A61L2/10; A61M1/3616; A61L2103/00; A61L2103/05; A61M2205/00; A61M2205/05; A61M2205/051; A61M2205/053
Claims Analysis
Total Claims: 39
Claim 1
Independent Claim
62 words
The method of irradiating a layer of a blood product containing white blood cells with ultraviolet radiation predominately of a wavelength of 280 to 320 nanometers, to provide a total energy exposure of 800 to 20,000 millijoules per square cm. of ultraviolet radiation, whereby said white blood cells substantially lose their capability to set off an immune reaction in an alloimmunized patient.
Claim 2
Independent Claim
62 words
The method of irradiating a layer of a blood product containing white blood cells with ultraviolet radiation predominately of a wavelength of 280 to 320 nanometers, to provide a total energy exposure of 800 to 20,000 millijoules per square cm. of ultraviolet radiation, whereby said white blood cells substantially lose their capability to set off an immune reaction in an alloimmunized patient.
Claim 3
Dependent Claim
23 words
The method of claim 1 in which said blood product is irradiated while occupying a flat, flexible bag made of poly(ethylene-vinyl acetate) plastic.
Claim 4
Dependent Claim
34 words
The method of claim 2 in which said plastic contains from 10 to 30 weight percent by weight of vinyl acetate units and said bag has a wall thickness of 0.005 to 0.025 inch.
Claim 5
Dependent Claim
19 words
The method of claim 1 in which said layer of blood product is from 0.1 to 5 cm. thick.
Claim 6
Dependent Claim
16 words
The method of claim 1 in which said irradiation is from 0.25 to 30 minutes long.
Claim 7
Dependent Claim
35 words
The method of claim 1 in which said blood product is irradiated while occupying a flat, flexible bag which is stretched during said irradiation to define a thin film of blood product within said bag.
Claim 8
Dependent Claim
23 words
The method of claim 1 in which the total energy exposure of ultraviolet radiation applied is 10,000 to 14,000 millijoules per square cm.
Claim 9
Dependent Claim
19 words
The method of claim 1 in which said total energy exposure is 1600 to 2500 millijoules per square cm.
Claim 10
Dependent Claim
21 words
The method of claim 1 in which the intensity of said ultraviolet radiation is 4 to 20 milliwatts per square cm.
Claim 11
Dependent Claim
17 words
The method of claim 1 in which said intensity is 10 to 15 milliwatts per square cm.
Claim 12
Dependent Claim
19 words
The method of claim 7 in which said irradiating takes place without agitation of the layer of blood product.
Claim 13
Dependent Claim
20 words
The method of claim 1 in which said ultraviolet radiation is predominately of a wavelength of 300 to 320 nanometers.
Claim 14
Independent Claim
108 words
The method of irradiating a layer of blood product containing white blood cells with ultraviolet radiation predominately of a wavelength of 280 to 320 nanometers, which comprises: presenting said blood product to the radiation while said blood product occupies a flat, flexible bag made of poly(ethylene-vinyl acetate) plastic, said plastic containing from 10 to 30 percent of vinyl acetate units, and said bag having a wall thickness of 0.005 to 0.025 inch, to provide a total energy exposure of 800 to 20,000 millijoules per square cm. of ultraviolet radiation, whereby said white blood cells substantially lose their capability to set off an immune reaction in an alloimmunized patient.
Claim 15
Independent Claim
108 words
The method of irradiating a layer of blood product containing white blood cells with ultraviolet radiation predominately of a wavelength of 280 to 320 nanometers, which comprises: presenting said blood product to the radiation while said blood product occupies a flat, flexible bag made of poly(ethylene-vinyl acetate) plastic, said plastic containing from 10 to 30 percent of vinyl acetate units, and said bag having a wall thickness of 0.005 to 0.025 inch, to provide a total energy exposure of 800 to 20,000 millijoules per square cm. of ultraviolet radiation, whereby said white blood cells substantially lose their capability to set off an immune reaction in an alloimmunized patient.
Claim 16
Dependent Claim
26 words
The method of claim 13 in which said bag is axially stretched during said irradiation to define said thin film of blood product within said bag.
Claim 17
Dependent Claim
20 words
The method of claim 13 in which said layer of white blood cells is from 0.1 to 5 cm. thick.
Claim 18
Independent Claim
96 words
The method of irradiating a layer of blood product containing white blood cells with ultraviolet radiation predominately of a wavelength between 280 to 320 nanometers at an intensity of 9 to 20 milliwatts per square cm., with agitation of the film, to provide a total energy exposure of 800 to 2800 millijoules per square cm. of ultraviolet radiation, in which said blood product is irradiated while occupying a flat, flexible bag which is stretched during said irradiation to define said layer of white blood cells within said bag, said bag being made of poly(ethylene-vinyl acetate) plastic.
Claim 19
Independent Claim
96 words
The method of irradiating a layer of blood product containing white blood cells with ultraviolet radiation predominately of a wavelength between 280 to 320 nanometers at an intensity of 9 to 20 milliwatts per square cm., with agitation of the film, to provide a total energy exposure of 800 to 2800 millijoules per square cm. of ultraviolet radiation, in which said blood product is irradiated while occupying a flat, flexible bag which is stretched during said irradiation to define said layer of white blood cells within said bag, said bag being made of poly(ethylene-vinyl acetate) plastic.
Claim 20
Dependent Claim
31 words
The method of claim 16 which said plastic contains from 10 to 30 weight percent of vinyl acetate units, and said bag has a wall thickness of 0.005 to 0.025 inch.
Claim 21
Dependent Claim
16 words
The method of claim 16 in which said irradiation is from 10 to 30 minutes long.
Claim 22
Dependent Claim
20 words
The method of claim 16 in which said layer of white blood cells is from 1 to 4 cm. thick.
Claim 23
Dependent Claim
66 words
Apparatus for irradiating with ultraviolet radiation a layer containing white blood cells, which comprises: means for providing ultraviolet radiation predominately of a wavelength of 280 to 320 nanometers at an intensity of 9 to 20 milliwatts per square cm.; means for supporting a layer of white blood cells in a position to be irradiated by said irradiation means, and means for initiating and terminating said irradiation.
Claim 24
Dependent Claim
66 words
Apparatus for irradiating with ultraviolet radiation a layer containing white blood cells, which comprises: means for providing ultraviolet radiation predominately of a wavelength of 280 to 320 nanometers at an intensity of 9 to 20 milliwatts per square cm.; means for supporting a layer of white blood cells in a position to be irradiated by said irradiation means, and means for initiating and terminating said irradiation.
Claim 25
Dependent Claim
28 words
The apparatus of claim 20 which includes means for stretching a flat, flexible bag containing said white blood cells during said irradiation to assist in forming said layer.
Claim 26
Dependent Claim
22 words
The apparatus of claim 21, in combination with a flat, flexible bag made of poly(ethylene-vinyl acetate) plastic containing said white blood cells.
Claim 27
Dependent Claim
33 words
The apparatus of claim 22 in which said plastic contains from 10 to 30 percent by weight of vinyl acetate units, and said bag has a wall thickness of 0.005 to 0.025 inch.
Claim 28
Dependent Claim
20 words
The apparatus of claim 23 in which said layer of white blood cells is from 0.1 to 5 cm. thick.
Claim 29
Dependent Claim
131 words
Apparatus for irradiating with ultraviolet radiation a layer of blood product containing white blood cells, which comprises: a housing; means for providing ultraviolet radiation; means for supporting in said housing a layer of white blood cells in a position to be irradiated by said irradiation means, said supporting means comprising a drawer member slideable into and out of said housing to carry an ultraviolet transmissive container which defines said layer, said drawer member defining a container-supporting ultraviolet-transmissive surface including at least part of a bottom to said drawer, said drawer bottom being pivotally attached to the remainder of said drawer member, wherein said surface permits ultraviolet radiation from said ultraviolet providing means to pass through the container-supporting surface and said container carried thereon, to irradiate said layer containing white blood cells.
Claim 30
Dependent Claim
131 words
Apparatus for irradiating with ultraviolet radiation a layer of blood product containing white blood cells, which comprises: a housing; means for providing ultraviolet radiation; means for supporting in said housing a layer of white blood cells in a position to be irradiated by said irradiation means, said supporting means comprising a drawer member slideable into and out of said housing to carry an ultraviolet transmissive container which defines said layer, said drawer member defining a container-supporting ultraviolet-transmissive surface including at least part of a bottom to said drawer, said drawer bottom being pivotally attached to the remainder of said drawer member, wherein said surface permits ultraviolet radiation from said ultraviolet providing means to pass through the container-supporting surface and said container carried thereon, to irradiate said layer containing white blood cells.
Claim 31
Dependent Claim
95 words
The apparatus of claim 25 in which said drawer member is movable between an open position permitting placement and removal of said container into and out of said drawer member, and a closed position in which said container in the drawer member is positioned within said housing for ultraviolet irradiation, and elevating means positioned to cause back-and-forth pivoting of said drawer bottom while said drawer member is in said closed position, to permit agitation of said layer containing white blood cells in said ultraviolet-transmissive container carried on said drawer bottom simultaneously with ultraviolet irradiation thereof.
Claim 32
Dependent Claim
21 words
The apparatus of claim 26 in which said elevating means comprises a rotatable, eccentric cam upon which said drawer bottom rests.
Claim 33
Dependent Claim
135 words
Apparatus for irradiating with ultraviolet radiation a layer of blood product containing white blood cells, which comprises: a housing; means for providing ultraviolet radiation; means for supporting a layer of white blood cells in a position to be irradiated by said irradiation means, said supporting means comprising a drawer member slideable into and out of said housing to carry an ultraviolet transmissive container which defines said layer, said drawer member defining a container-supporting surface, said container-supporting surface of the drawer member being pivotally attached to the remainder of said drawer member; and elevating means positioned to cause back-and-forth pivoting of said drawer bottom when the drawer member is in closed position, to permit agitation of said layer containing white blood cells in said ultraviolet-transmissive container carried on said drawer bottom simultaneously with ultraviolet irradiation thereof.
Claim 34
Dependent Claim
135 words
Apparatus for irradiating with ultraviolet radiation a layer of blood product containing white blood cells, which comprises: a housing; means for providing ultraviolet radiation; means for supporting a layer of white blood cells in a position to be irradiated by said irradiation means, said supporting means comprising a drawer member slideable into and out of said housing to carry an ultraviolet transmissive container which defines said layer, said drawer member defining a container-supporting surface, said container-supporting surface of the drawer member being pivotally attached to the remainder of said drawer member; and elevating means positioned to cause back-and-forth pivoting of said drawer bottom when the drawer member is in closed position, to permit agitation of said layer containing white blood cells in said ultraviolet-transmissive container carried on said drawer bottom simultaneously with ultraviolet irradiation thereof.
Claim 35
Dependent Claim
21 words
The apparatus of claim 28 in which said elevating means comprises a rotatable, eccentric cam upon which said drawer bottom rests.
Claim 36
Dependent Claim
109 words
Apparatus for irradiating with ultraviolet radiation a layer of blood product containing white blood cells, which comprises: a housing; means for providing ultraviolet radiation; means for supporting in said housing a layer of white blood cells in a position to be irradiated by said irradiation means, said supporting means comprising a drawer member slideable into and out of said housing to carry an ultraviolet transmissive container which defines said layer, said drawer member defining a container-supporting ultraviolet-transmissive surface made of quartz, to permit ultraviolet radiation from said ultraviolet providing means to pass through the container-supporting surface and said container carried thereon, to irradiate said layer containing white blood cells.
Claim 37
Dependent Claim
109 words
Apparatus for irradiating with ultraviolet radiation a layer of blood product containing white blood cells, which comprises: a housing; means for providing ultraviolet radiation; means for supporting in said housing a layer of white blood cells in a position to be irradiated by said irradiation means, said supporting means comprising a drawer member slideable into and out of said housing to carry an ultraviolet transmissive container which defines said layer, said drawer member defining a container-supporting ultraviolet-transmissive surface made of quartz, to permit ultraviolet radiation from said ultraviolet providing means to pass through the container-supporting surface and said container carried thereon, to irradiate said layer containing white blood cells.
Claim 38
Dependent Claim
129 words
Apparatus for irradiating with ultraviolet radiation a layer of blood product containing white blood cells, which comprises: a housing; means for providing ultraviolet radiation; means for supporting in said housing a layer of white blood cells in a position to be irradiated by said irradiation means, said supporting means comprising a drawer member slideable into and out of said housing to carry an ultraviolet transmissive container which defines said layer, said drawer member defining a container-supporting ultraviolet-transmissive surface to permit ultraviolet radiation from said ultraviolet providing means to pass through the container-supporting surface and said container carried thereon, to irradiate said layer containing white blood cells, said means for providing ultraviolet radiation also providing said radiation directly to a side of said container facing away from the container-supporting surface.
Claim 39
Dependent Claim
129 words
Apparatus for irradiating with ultraviolet radiation a layer of blood product containing white blood cells, which comprises: a housing; means for providing ultraviolet radiation; means for supporting in said housing a layer of white blood cells in a position to be irradiated by said irradiation means, said supporting means comprising a drawer member slideable into and out of said housing to carry an ultraviolet transmissive container which defines said layer, said drawer member defining a container-supporting ultraviolet-transmissive surface to permit ultraviolet radiation from said ultraviolet providing means to pass through the container-supporting surface and said container carried thereon, to irradiate said layer containing white blood cells, said means for providing ultraviolet radiation also providing said radiation directly to a side of said container facing away from the container-supporting surface.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-08-27 | 1994-02-26 | 1994-08-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-08-27 | 1998-02-26 | 1998-08-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-08-27 | 2002-02-26 | 2002-08-28 | $7700.00 | Window Closed / Lapsed |