US4608255A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biocompatible method for in situ production of functional platelets and product produced thereby lacking immunogenicity
Assignee: American Red Cross
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Filing: 1985-01-31
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Granted: 1986-08-26
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Expires: 2005-01-31
Abstract
Abstract The present invention discloses the effect of ultraviolet (UV) radiation on platelet concentrates collected in a plastics container, particularly in a polypropylene bag. Samples irradiated at 310 nm for 30 minutes at a dose of 774 J/M 2 show no loss of platelet function as determined by ADP, collagen, or ristocetin-induced aggregation. Lymphocytes isolated from irradiated units are unable to act as responders or stimulators in a mixed lymphocyte reaction. The invention provides a method of UV irradiation of platelet concentrates resulting in transfusible cell suspension unable to evoke immunological response while retaining normal platelet function.
Inventors
- 👤 Richard A. Kahn
- 👤 Glenn E. Rodey
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 16:20
CPC Classifications
A; A61; A61M; A61M1/00; A61M1/36; A61M1/3681; A61M1/3616; A61M2205/00; A61M2205/05; A61M2205/051; A61M2205/053
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
47 words
An isolated, immunogen-free, transfusible platelet suspension in an enclosed, inert, biocompatible container permeable to ultraviolet radiation, said platelet suspension being biologically functional and having been rendered free of immunogenicity in situ by treatment with an effective amount of ultraviolet radiation external to and permeable through said container.
Claim 2
Dependent Claim
47 words
An isolated, immunogen-free, transfusible platelet suspension in an enclosed, inert, biocompatible container permeable to ultraviolet radiation, said platelet suspension being biologically functional and having been rendered free of immunogenicity in situ by treatment with an effective amount of ultraviolet radiation external to and permeable through said container.
Claim 3
Dependent Claim
37 words
A container enclosably containing an in situ ultraviolet-irradiated, transfusible, functional platelet suspension lacking immunogenicity, said container being made of an inert, biocompatible material suitable for passage of ultraviolet radiation therethrough from a source external to said container.
Claim 4
Dependent Claim
37 words
A container enclosably containing an in situ ultraviolet-irradiated, transfusible, functional platelet suspension lacking immunogenicity, said container being made of an inert, biocompatible material suitable for passage of ultraviolet radiation therethrough from a source external to said container.
Claim 5
Dependent Claim
24 words
The container of claim 2 wherein said container is formed of a material selected from the group consisting of polypropylene, polyethylene and polyvinyl chloride.
Claim 6
Independent Claim
78 words
A method of in situ producing functional platelets lacking immunogenicity in a container containing platelets comprising collecting a platelet suspension in an inert, biocompatible, sterile, closed container made of a material suitable for passage of ultraviolet radiation therethrough and then irradiating said platelet suspension with an external source of ultraviolet radiation capable of penetrating through said container for a time and at a dosage level sufficient to inactivate immunogenic factors present in said suspension without affecting platelet function.
Claim 7
Independent Claim
78 words
A method of in situ producing functional platelets lacking immunogenicity in a container containing platelets comprising collecting a platelet suspension in an inert, biocompatible, sterile, closed container made of a material suitable for passage of ultraviolet radiation therethrough and then irradiating said platelet suspension with an external source of ultraviolet radiation capable of penetrating through said container for a time and at a dosage level sufficient to inactivate immunogenic factors present in said suspension without affecting platelet function.
Claim 8
Dependent Claim
24 words
The method of claim 4 wherein said container is formed of plastics material selected from the group consisting of polypropylene, polyethylene and polyvinyl chloride.
Claim 9
Dependent Claim
20 words
The method of claim 5 wherein the dosage of said radiation is about 645 Joules/M 2 for about 10-40 minutes.
Claim 10
Dependent Claim
13 words
A transfusible platelet suspension lacking immunogenicity produced by the method of claim 6.
References Cited (Backward Citations)
US2401131A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-08-25 | 1990-02-24 | 1990-08-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-08-25 | 1994-02-24 | 1994-08-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-08-25 | 1998-02-24 | 1998-08-26 | $7700.00 | Window Closed / Lapsed |