Patent US4904584A successfully refreshed from Google Patents.
US4904584A
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Site-specific homogeneous modification of polypeptides
Assignee: Genetics Institute LLC
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Filing: 1987-12-23
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Granted: 1990-02-27
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Expires: 2007-12-23
Abstract
Abstract A homogeneously modified protein is provided having one or more selected naturally occurring lysine residues replaced by a suitable amino acid, or having one or more lysine residues substituted for other amino acids or inserted into a polypeptide sequence, leaving selected lysine residues having ε-amino groups in the protein and coupling amine reactive compounds to selected lysine residues. Methods for producing the selected homogeneously modified proteins and pharmaceutical compositions containing such proteins are provided.
Inventors
- 👤 Gray Shaw
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 06:11
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/53; C07K14/535; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/56; A61K47/59; A61K47/60; C07K14/54; C07K14/5403; C07K14/5409; C07K14/55; C12; C12N; C12N15/00; C12N15/09; C12N15/11; A61K38/00
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
47 words
A polypeptide selected from the group consisting of G-CSF and erythropoietin having one or more lysine residues deleted, or replaced with an amino acid other than lysne, and containing at least one polyalkylene glycol moiety covalently attached to at least one amino acid residue of the polypeptide.
Claim 2
Dependent Claim
47 words
A polypeptide selected from the group consisting of G-CSF and erythropoietin having one or more lysine residues deleted, or replaced with an amino acid other than lysne, and containing at least one polyalkylene glycol moiety covalently attached to at least one amino acid residue of the polypeptide.
Claim 3
Dependent Claim
70 words
A method for preparing a polypeptide of claim 1 which comprises producing a modified form of the polypeptide in which one or more lysine residues are deleted or replaced with an amino acid other than lysine, reacting the modified polypeptide so produced with a polyalkylene glycol moiety under conditions permitting the covalent attachment of the moiety to the modified polypeptide, and recovering the product so produced from the reaction mixture.
Claim 4
Dependent Claim
19 words
A pharmaceutical composition containing a therapeutically effective amount of a polypeptide of claim 1 and a pharmaceutically acceptable carrier.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-02-26 | 1993-08-28 | 1994-02-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-02-26 | 1997-08-28 | 1998-02-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-02-26 | 2001-08-28 | 2002-02-27 | $7700.00 | Window Closed / Lapsed |