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Patent US4845035A successfully refreshed from Google Patents.
US4845035A
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Enzyme immobilization with a hydrolyzed polysaccharide graft copolymer
Assignee: United States Department of Agriculture
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Filing: 1987-10-06
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Granted: 1989-07-04
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Expires: 2007-10-06
Abstract
Abstract Immobilized enzyme-polysaccharide graft copolymer products are prepared by hydrolyzing a polysaccharide graft copolymer and then contacting the hydrolyzed copolymer with an enzyme to immobilize the enzyme on the copolymer. The copolymer-enzyme products retain large quantities of active enzyme, and activity of the enzyme is retained over long periods of use. In the case of glucoamylase immobilized in accordance with the method of the invention, improved yield of glucose from starch is obtained.
Inventors
- 👤 George Fanta
- 👤 Patricia J. Slininger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 18:30
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/10; C12N11/12; C12N11/08; C12N11/082; C12N11/087; C12P; C12P19/00; C12P19/14; C12P19/20
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
81 words
A method for preparing a polysaccharide graft copolymer-enzyme product, which consists essentially of: (a) hydrolyzing a polysaccharide graft copolymer, prepared by reaction of a vinyl or acrylic monomer with a polysaccharide, to remove a portion of said polysaccharide in said polysaccharide graft copolymer and obtain a final polysaccharide content in said hydrolyzed polysaccharide graft copolymer of 2 to 70%; and (b) contacting said hydrolyzed polysaccharide graft copolymer with an enzyme for a time sufficient to bind the enzyme to the copolymer.
Claim 2
Independent Claim
81 words
A method for preparing a polysaccharide graft copolymer-enzyme product, which consists essentially of: (a) hydrolyzing a polysaccharide graft copolymer, prepared by reaction of a vinyl or acrylic monomer with a polysaccharide, to remove a portion of said polysaccharide in said polysaccharide graft copolymer and obtain a final polysaccharide content in said hydrolyzed polysaccharide graft copolymer of 2 to 70%; and (b) contacting said hydrolyzed polysaccharide graft copolymer with an enzyme for a time sufficient to bind the enzyme to the copolymer.
Claim 3
Dependent Claim
20 words
The method of claim 1 wherein said final polysaccharide content in said hydrolyzed polysaccharide graft copolymer is 15 to 40%.
Claim 4
Dependent Claim
24 words
The method of claim 1 wherein said polysaccharide in said polysaccharide graft copolymer is selected from the group consisting of starch, cellulose, and xylan.
Claim 5
Dependent Claim
36 words
The method of claim 1 wherein said vinyl or acrylic monomer used to prepare said polysaccharide graft copolymer is selected from the group consisting of acrylonitrile, methyl acrylate, methyl methacrylate, ethyl acrylate, butyl acrylate, and styrene.
Claim 6
Dependent Claim
12 words
The method of claim 1 wherein said enzyme is a polysaccharide-degrading enzyme.
Claim 7
Dependent Claim
22 words
The method of claim 5 wherein said polysaccharide-degrading enzyme is selected from the group consisting of α-amylase, β-amylase, glucoamylase, cellulase, and xylanase.
Claim 8
Dependent Claim
19 words
The method of claim 1 wherein said polysaccharide graft copolymer is starch graft polyacrylonitrile and said enzyme is glucoamylase.
Claim 9
Dependent Claim
14 words
A polysaccharide graft copolymer-enzyme product prepared in accordance with the method of claim 1.
Claim 10
Dependent Claim
14 words
A polysaccharide graft copolymer-enzyme product prepared in accordance with the method of claim 2.
Claim 11
Dependent Claim
14 words
A polysaccharide graft copolymer-enzyme product prepared in accordance with the method of claim 3.
Claim 12
Dependent Claim
14 words
A polysaccharide graft copolymer-enzyme product prepared in accordance with the method of claim 4.
Claim 13
Dependent Claim
14 words
A polysaccharide graft copolymer-enzyme product prepared in accordance with the method of claim 5.
Claim 14
Dependent Claim
14 words
A polysaccharide graft copolymer-enzyme product prepared in accordance with the method of claim 6.
Claim 15
Dependent Claim
14 words
A polysaccharide graft copolymer-enzyme product prepared in accordance with the method of claim 7.
References Cited (Backward Citations)
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US4134863A
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US4194998A
Cited
US4338401A
Cited
US4483950A
Cited
US4663163A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-07-03 | 1993-01-02 | 1993-07-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-07-03 | 1997-01-02 | 1997-07-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-07-03 | 2001-01-02 | 2001-07-04 | $7700.00 | Window Closed / Lapsed |