US4829010A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immunoassay device enclosing matrixes of antibody spots for cell determinations
Assignee: Tanox Inc
•
Filing: 1987-03-13
•
Granted: 1989-05-09
•
Expires: 2007-03-13
Abstract
Abstract An immunoassay device comprising a support which has a substantially planar surface 14 on which is located an array of small, closely-spaced, discrete, antibody coated areas (spots) 17. A cover 16 is spaced from the support surface 14 and is positioned over the array of antibody coated spots 17. The cover is attached to the support surface to provide an enclosed chamber 30. In the cover there is at least one aperture communicating with the interior of the chamber 30 for introducing a liquid sample into the chamber. In addition, there is at least one additional aperture 34 in the cover, which also communicates with the interior of the chamber to allow air to escape upon introduction of the sample into the chamber. When a cell sample is introduced into the chamber it immediately fills the chamber and cells will settle uniformly over the matrix. The antibody-coated spots function as tiny immunoadsorbents for cells bearing antigens recognized by the antibody. The device can be used to determine the proportion of cells in a sample cell population.
Inventors
- 👤 Tse W. Chang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 06:03
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/508; B01L3/502; B01L3/5027; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; G01N33/551; G01N33/552; B01J; B01J2219/00; B01J2219/00274; B01J2219/00583; B01J2219/00603; B01J2219/00605; B01J2219/0061; B01J2219/00612; B01J2219/00614; B01J2219/00617; B01J2219/00623; B01J2219/0063; B01J2219/00632; B01J2219/00637; B01J2219/00659; B01L2200/00; B01L2200/06; B01L2200/0689; B01L2300/00; B01L2300/06; B01L2300/0627; B01L2300/0636; B01L2300/08; B01L2300/0809; B01L2300/0822; B01L2400/00; B01L2400/04; B01L2400/0403; B01L2400/0406; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/809
Claims Analysis
Total Claims: 39
Claim 1
Independent Claim
148 words
An immunoassay device comprising: a. a support having substantially planar, unrestricted surface, b. an array of small, closely-spaced, discrete, antibody-coated areas on the support surface; c. a cover spaced from the support surface and positioned over an array of antibody-coated areas; the cover being secured to the support surface to provide a single, unrestricted, enclosed chamber, the cover having at least one aperture communicating with the interior of the single chamber and with ambient atmosphere for introducing a liquid sample into the chamber to flow across the planar surface without impediment and into engagement with all of said coated areas regardless of the location of the aperture relative to the planar surface, and at least one additional aperture in the cover also communicating with the interior of the chamber and ambient atmosphere to allow air to escape from the chamber upon introduction of the sample into the chamber.
Claim 2
Dependent Claim
148 words
An immunoassay device comprising: a. a support having substantially planar, unrestricted surface, b. an array of small, closely-spaced, discrete, antibody-coated areas on the support surface; c. a cover spaced from the support surface and positioned over an array of antibody-coated areas; the cover being secured to the support surface to provide a single, unrestricted, enclosed chamber, the cover having at least one aperture communicating with the interior of the single chamber and with ambient atmosphere for introducing a liquid sample into the chamber to flow across the planar surface without impediment and into engagement with all of said coated areas regardless of the location of the aperture relative to the planar surface, and at least one additional aperture in the cover also communicating with the interior of the chamber and ambient atmosphere to allow air to escape from the chamber upon introduction of the sample into the chamber.
Claim 3
Dependent Claim
21 words
An immunoassay device of claim 1, wherein all of the discrete, antibody-coated areas are of substantially the same size and shape.
Claim 4
Dependent Claim
24 words
An immunoassay device of claim 1 wherein the antibodies in the discrete antibody-coated surface areas are covalently bound to the surface of the support.
Claim 5
Dependent Claim
34 words
An immunoassay device according to claim 1, wherein the antibody in each of the discrete, antibody-coated surface areas, or in identifiable sets of said antibody-corated surface areas, is antibody of different and distinct specificity.
Claim 6
Dependent Claim
20 words
An immunoassay device according to claim 1, wherein the antibodies in the discrete, antibody-coated areas are highly purified polyclonal antibodies.
Claim 7
Dependent Claim
18 words
An immunoassay device according to claim 1, wherein the antibodies in the discrete, antibody-coated areas are monoclonal antibodies.
Claim 8
Dependent Claim
15 words
An immunoassay device according to claim 1, wherein the support is a glass microscope slide.
Claim 9
Dependent Claim
15 words
An immunoassay device according to claim 1, wherein the support is a plastic microscope slide.
Claim 10
Dependent Claim
25 words
An immunoassay device according to claim 1, wherein discrete, antibody-coated surface areas are dots having diameters of from about 0.25 mm to about 1.0 mm.
Claim 11
Dependent Claim
29 words
An immunoassay device according to claim 1, wherein said antibody-coated dots on the surface of the support are arranged in a compact 5-20 dot by 5-20 dot rectangular matrix.
Claim 12
Dependent Claim
76 words
An immunoassay device of claim 1, wherein the antibodies are adsorbed to the planar surface at a density and uniformity such that when cells bearing a surface antigen are brought into contact in sufficient concentration with a discrete antibody-coated area containing antibodies which bind the antigen, the cells adhere tightly to the discrete, antibody coated area and form a layer of bound cells which is substantially microscopically uniform over essentially all of the discrete antibody-coated area.
Claim 13
Dependent Claim
30 words
An immunoassay device of claim 1, wherein the antibody coated areas are formed by applying solutions of antibody to the support surface of from about 10 to about 20 μg/ml.
Claim 14
Dependent Claim
18 words
An immunoassay device of claim 1, wherein the cover is detachably secured to the surface of the support.
Claim 15
Dependent Claim
20 words
An immunoassay device of claim 1, wherein at least two arrays of antibody coated areas are located on the support.
Claim 16
Dependent Claim
18 words
An immunoassay device according to claim 1, wherein the array of discrete, antibody-coated areas is a rectangular matrix.
Claim 17
Dependent Claim
32 words
An immunoassay device according to claim 15, wherein the entire rectangular matrix is contained with an area of from about 0.5 to about 1.0 cm 2 of the surface of the support.
Claim 18
Dependent Claim
32 words
An immunoassay device of claim 1, wherein the cover is substantially rectangular and planar and is mounted upon walls which are secured to the surface of the support and extend upwardly therefrom.
Claim 19
Dependent Claim
25 words
An immunoassay device of claim 17, wherein the walls are secured to the planar support by projections extending between the wall and the planar surface.
Claim 20
Dependent Claim
30 words
An immunoassay device of claim 1, wherein the surface of the planar support is coated with a visible, inert, water repellant, material except where the antibody coated spots are located.
Claim 21
Dependent Claim
15 words
An immunoassay device of claim 19, wherein the coating material is tetrafluoroethylene or fluorinated ethylene-propylene.
Claim 22
Dependent Claim
159 words
An immunoassay device comprising: a. a support having a substantially planar, unrestricted surface, b. an array of small, discrete, closely-spaced, antibody-coated areas on the planar surface; c. walls extending upwardly from the support surface and secured to the surface to form a single rectangular enclosure located at the perimeter of the array of antibody-coated areas and defining the planar unrestricted surface within the walls, and d. a cover mounted on the walls thereby providing a single, unrestricted enclosed chamber, the cover having at least one aperture communicating with the interior of the chamber interior for introduction a sample into the chamber to flow across the planar surface without impediment and into engagement with all of said coated areas, and at least one other aperture also communicating with the chamber interior to permit the escape of air from the chamber when a sample is introduced into the chamber regardless of the location of either aperture relative to the planar surface.
Claim 23
Dependent Claim
159 words
An immunoassay device comprising: a. a support having a substantially planar, unrestricted surface, b. an array of small, discrete, closely-spaced, antibody-coated areas on the planar surface; c. walls extending upwardly from the support surface and secured to the surface to form a single rectangular enclosure located at the perimeter of the array of antibody-coated areas and defining the planar unrestricted surface within the walls, and d. a cover mounted on the walls thereby providing a single, unrestricted enclosed chamber, the cover having at least one aperture communicating with the interior of the chamber interior for introduction a sample into the chamber to flow across the planar surface without impediment and into engagement with all of said coated areas, and at least one other aperture also communicating with the chamber interior to permit the escape of air from the chamber when a sample is introduced into the chamber regardless of the location of either aperture relative to the planar surface.
Claim 24
Dependent Claim
21 words
An immunoassay device of claim 21, wherein all of the discrete, antibody-coated areas are of substantially the same size and shape.
Claim 25
Dependent Claim
24 words
An immunoassay device of claim 21, wherein the antibodies in the discrete antibody-coated surface areas are covalently bound to the surface of the support.
Claim 26
Dependent Claim
34 words
An immunoassay device according to claim 21, wherein the antibody in each of the discrete, antibody-coated surface areas, or in identifiable sets of said antibody-coated surface areas, is antibody of different and distinct specificty.
Claim 27
Dependent Claim
21 words
An immunoassay device according to claim 21, wherein the antibodies in the discrete, antibody-coated surface areas are highly purified polyclonal antibodies.
Claim 28
Dependent Claim
18 words
An immuncassay device according to claim 21, wherein the antibodies in the discrete, antibody-coated areas are monoclonal antibodies.
Claim 29
Dependent Claim
17 words
An immunoassay device according to claim 21, wherein the support is a glass or plastic microscope slides.
Claim 30
Dependent Claim
18 words
An immunoassay device according to claim 21, wherein the array of discrete, antibody-coated areas is a rectangular matrix.
Claim 31
Dependent Claim
25 words
An immunoassay device according to claim 21, wherein discrete, antibody-coated surface areas are dots having diameters of from about 0.25 mm to about 1.0 mm.
Claim 32
Dependent Claim
69 words
An immunoassay device of claim 21 wherein the antibodies are adsorbed to the planar surface at a density and uniformity such that when cells bearing a surface antigen are brought into contact in sufficient concentration wlth a discrete, antibody-coated area containing antibodies which bind the antigen, the cells adhere tightly to the discrete, antibody coated area and distribute substantially microscopically uniformly over essentially all of the discrete antibody-coated areas.
Claim 33
Dependent Claim
25 words
An immunoassay device of claim 21, wherein the antibody-coated areas are formed by applying solutions of antibody of from about 10 to about 20 μl/ml.
Claim 34
Dependent Claim
35 words
An immunoassay device of claim 21, wherein the surface of the planar support in the field of the antibody-coated areas is coated with a visible, inert layer except where the antibody coated spots are located.
Claim 35
Dependent Claim
18 words
An immunoassay device of claim 21, wherein the walls and cover are detachably secured to the support surface.
Claim 36
Dependent Claim
29 words
An immunoassay device according to claim 21, wherein said antibody-coated dots on the surface of the support are arranged in a compact 5-20 dot by 5-20 dot rectangular matrix.
Claim 37
Dependent Claim
32 words
An immunoassay device according to claim 34, wherin the entire rectangular matrix is contained within an area of from about 0.5 to about 1.0 cm 2 of the surface of the support.
Claim 38
Dependent Claim
114 words
An immunoassay device, comprising: a. a glass or plastic support having a substantially planar, unrestricted surface; b. two, separate rectangular arrays of antibody-coated dots on the planar surface, the density of antibody in the dots being about 10 μg/ml to 20 μg/ml; c. walls extending upwardly from the support surface and located at the perimeter of each array of antibody-coated dots and defining the planar, unrestricted surface within the walls; and d. a cover mounted upon the walls thereby forming an enclosed chamber over each array, the cover having a sample injection aperture communicating with the chamber space and a plurality of apertures also communicating with the chamber space to permit air to escape.
Claim 39
Dependent Claim
114 words
An immunoassay device, comprising: a. a glass or plastic support having a substantially planar, unrestricted surface; b. two, separate rectangular arrays of antibody-coated dots on the planar surface, the density of antibody in the dots being about 10 μg/ml to 20 μg/ml; c. walls extending upwardly from the support surface and located at the perimeter of each array of antibody-coated dots and defining the planar, unrestricted surface within the walls; and d. a cover mounted upon the walls thereby forming an enclosed chamber over each array, the cover having a sample injection aperture communicating with the chamber space and a plurality of apertures also communicating with the chamber space to permit air to escape.
References Cited (Backward Citations)
US3165450A
Cited
US4317810A
Cited
US4154795A
Cited
US4055394A
Cited
US4216245A
Cited
US4228127A
Cited
US4315907A
Cited
US4357142A
Cited
EP0063810A1
Cited
US4471056A
Cited
US4468371A
Cited
JPS5994068A
Cited
WO1984003151A1
Cited
US4591570A
Cited
US4708933A
Cited
EP0200507A1
Cited
US4647430A
Cited
US4761381A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-05-08 | 1992-11-07 | 1993-05-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-05-08 | 1996-11-07 | 1997-05-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-05-08 | 2000-11-07 | 2001-05-09 | $7700.00 | Window Closed / Lapsed |