US4471056A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for HLA-DR typing of total human lymphocyte sample
Assignee: Leland Stanford Junior University
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Filing: 1982-04-02
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Granted: 1984-09-11
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Expires: 2002-04-02
Abstract
Abstract An HLA-DR typing test based on lymphocytotoxicity in which a vital dye-labeled total human lymphocyte sample, such as a sample of peripheral blood lymphocytes, is incubated with HLA-DR antisera, a monoclonal antibody against T cells, and complement and the DR type is determined based upon the resultant cytotoxicity as measured by the fluorescence of B cells surviving the incubation.
Inventors
- 👤 Frank C. Grumet
- 👤 Edgar G. Engleman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 09:48
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56966; G01N33/56977; G01N33/5005; G01N33/5091; Y; Y10; Y10S; Y10S436/00; Y10S436/821
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
69 words
An HLA-DR typing method comprising: (a) incubating a total human lymphocyte sample with (i) an anti-HLA-DR antibody that activates complement; (ii) and anti-human T cell antibody that activates complement; and (iii) complement; and (b) determining whether the HLA-DR antigen to which the anti-HLA-DR antibody binds is present in the B cells in the sample by comparing the viable B cell content of the incubated sample with a negative control.
Claim 2
Dependent Claim
69 words
An HLA-DR typing method comprising: (a) incubating a total human lymphocyte sample with (i) an anti-HLA-DR antibody that activates complement; (ii) and anti-human T cell antibody that activates complement; and (iii) complement; and (b) determining whether the HLA-DR antigen to which the anti-HLA-DR antibody binds is present in the B cells in the sample by comparing the viable B cell content of the incubated sample with a negative control.
Claim 3
Dependent Claim
15 words
The method of claim 1 wherein the sample is a sample of peripheral blood lymphocytes.
Claim 4
Dependent Claim
17 words
The method of claim 1 or 2 wherein the anti-human T cell antibody is a monoclonal antibody.
Claim 5
Dependent Claim
14 words
The method of claim 3 wherein the monoclonal antibody is of the IgM class.
Claim 6
Dependent Claim
13 words
The method of claim 1 or 2 wherein the complement is rabbit complement.
Claim 7
Dependent Claim
35 words
The method of claim 1 or 2 wherein the sample is labeled with a vital dye before the incubation and the resultant cytotoxicity is based on the loss of the vital dye by lysed cells.
Claim 8
Dependent Claim
12 words
The method of claim 6 wherein the vital dye is carboxyfluorescein diacetate.
Claim 9
Dependent Claim
49 words
The method of claim 2 wherein the anti-human T cell antibody is a monoclonal antibody of the IgM class; the complement is rabbit complement; the sample is labeled with carboxyfluorescein diacetate before the incubation and the resultant cytotoxicity is based on the loss of the label by lysed cells.
Claim 10
Dependent Claim
30 words
The method of claim 1, 2, or 8 wherein the incubation of the sample with the HLA-DR antibody, anti-human T cell antibody, and complement is carried out in separate steps.
Claim 11
Dependent Claim
39 words
The method of claim 9 wherein the HLA-DR antibody incubation step and the anti-human T cell antibody incubation step are each carried out at about 37° C. and the complement incubation step is carried out at about 22° C.
References Cited (Backward Citations)
US4239746A
Cited
US4124701A
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US4328183A
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EP0017381A2
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EP0018795A2
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US4420558A
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US4403042A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-09-11 | 1988-03-12 | 1988-09-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-09-11 | 1992-03-12 | 1992-09-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-09-11 | 1996-03-12 | 1996-09-11 | $7700.00 | Window Closed / Lapsed |