US4124701A
Expired / Lapsed
US
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Method for preparing a reagent for PLT and method of use
Assignee: Wisconsin Alumni Research Foundation
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Filing: 1977-07-11
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Granted: 1978-11-07
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Expires: 1997-07-11
Abstract
Abstract A method for preparing a reagent for primed LD typing which comprises sensitizing purified human blood leukocytes to a secondary proliferative response by incubating them, in vitro, in mixed leukocyte culture, with lymphoblastoid cell lines from homozygous typing cells, or from other cells of specified genetic type homozygous for HLA-D, or other continuous line.
Inventors
- 👤 Marilyn L. Bach
- 👤 Fritz H. Bach
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 06:59
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56966; G01N33/56977; Y; Y10; Y10S; Y10S436/00; Y10S436/826
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
52 words
A method for preparation of a reagent for lymphocyte-defined typing of human leukocyte antigens which comprises incubating in vitro purified blood leukocytes with human cells selected from lymphoblastoid cell lines from homozygous typing cells, other cells homozygous for HLA-D, or other continuous line cells, and recovering the leukocytes from the incubation medium.
Claim 2
Independent Claim
52 words
A method for preparation of a reagent for lymphocyte-defined typing of human leukocyte antigens which comprises incubating in vitro purified blood leukocytes with human cells selected from lymphoblastoid cell lines from homozygous typing cells, other cells homozygous for HLA-D, or other continuous line cells, and recovering the leukocytes from the incubation medium.
Claim 3
Dependent Claim
27 words
A method according to claim 1 in which the leukocytes recovered from the incubation medium contains lymphocytes that have been primed to recognize given HLA-D region antigens.
Claim 4
Dependent Claim
20 words
A method according to claim 2 in which the incubation is carried out essentially to completion of the proliferative response.
Claim 5
Dependent Claim
24 words
A method according to claim 2 in which the incubation is carried out for a period of from about 9 to about 14 days.
Claim 6
Dependent Claim
15 words
A method according to claim 4 in which the incubation is terminated after 10 days.
Claim 7
Dependent Claim
17 words
A reagent for lymphocyte defined typing of human leukocyte antigens produced by the method of claim 1.
Claim 8
Dependent Claim
20 words
A reagent according to claim 6 which contains lymphocytes that have been primed to recognize a given HLA-D region antigen(s).
Claim 9
Dependent Claim
28 words
A method for assaying for antigenic compatibility comprising measuring the proliferative responsive of the primed lymphocytes of claim 7 to antigens of restimulating cells of a third person.
Claim 10
Dependent Claim
61 words
A method for typing human leukocyte antigens comprising incubating in vitro the reagent of claim 6 with purified human block leukocytes obtained from a third person, measuring the response of the recovered leukocytes through the uptake by the responding leukocytes of tritiated thymidine or tritiated uridine added to the incubating mixture, said measurement defining the antigens present in the third person.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-11-06 | 1982-05-08 | 1982-11-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-11-06 | 1986-05-08 | 1986-11-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-11-06 | 1990-05-08 | 1990-11-07 | $7700.00 | Window Closed / Lapsed |