Complement fixation test employing reactants in a disposable package

Claim 1

A process for carrying out a serological investigation in a disposable package comprising a chemically inert transparent reaction vessel for performing a serological complement fixation test which contains reactants for said test comprising an antigen or antibodies to the antigen to be detected, hemolytic amboceptor and complement, said reactants being preserved in said vessel in frozen or freeze-dried form, each having been deep frozen or freeze-dried to form a separate layer or having been deep frozen or freeze-dried in separate areas of said vessel wherein said process comprises bringing said reactants into solution by thawing or adding a solvent for suitable adjustment of proportions of said reactants, adding 0.25 ml. of the patient's serum, which may contain the respective antigen or antibodies to be detected, incubating the resulting solution at 37° C. to effect a binding reaction and to use up the complement with formation of antigen-antibody complex, adding 2% sheep's erythrocyte suspension, again incubating at 37° C., then assessing the degree of haemolysis.