US4317810A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Waffle-like matrix for immunoassay and preparation thereof
Assignee: Cordis Laboratories Inc
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Filing: 1980-03-02
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Granted: 1982-03-02
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Expires: 2000-03-02
Abstract
Abstract A disc-shaped insoluble matrix is formed of a polymeric material having a layer of reactive groups which react with proteins binding them covalently and uniformly to its surface. An antibody adapted for use in immunoassay of antigens associated with hepatitis is covalently bonded to the reactive groups. The matrix is provided with irregular, e.g., waffle-like, surfaces designed to increase the amount of antibody that will be in contact with a test solution such as serum.
Inventors
- 👤 Seymour P. Halbert
- 👤 Milton Anken
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 11:20
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/809; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
105 words
A material for use in immunoassay comprising a water insoluble polymeric matrix having a layer of reactive groups grafted into its surface, said reactive groups being capable of covalently bonding to protein to enable the structure to be used in immunoassay wherein the improvement comprises a designed configuration of top and bottom waffle-like surfaces having a plurality of ridges and depressions on said polymeric matrix which, when placed in a flat bottom vial, both top and bottom surfaces will be substantially in contact with any solution in the vial while minimizing the surface to surface contact between the matrix and the bottom of the vial.
Claim 2
Dependent Claim
105 words
A material for use in immunoassay comprising a water insoluble polymeric matrix having a layer of reactive groups grafted into its surface, said reactive groups being capable of covalently bonding to protein to enable the structure to be used in immunoassay wherein the improvement comprises a designed configuration of top and bottom waffle-like surfaces having a plurality of ridges and depressions on said polymeric matrix which, when placed in a flat bottom vial, both top and bottom surfaces will be substantially in contact with any solution in the vial while minimizing the surface to surface contact between the matrix and the bottom of the vial.
Claim 3
Dependent Claim
28 words
The material as set forth in claim 1 wherein an antibody reactive with antigens associated with hepatitis is covalently bonded to the reactive groups on said polymeric matrix.
Claim 4
Dependent Claim
15 words
The material of claim 1 wherein waffle-like surfaces include a series of linear parallel ridges.
Claim 5
Dependent Claim
34 words
A material in accordance with claim 3 wherein both waffle-like surfaces include a first series of linear ridges and a second series of linear ridges arranged perpendicular to said first series of linear ridges.
Claim 6
Dependent Claim
39 words
The material of claim 1 wherein said waffle-like surfaces are formed by passing the polymeric matrix through a pair of rollers having surface projections of a design to impart the waffle-like surface configuration onto the polymeric matrix by pressing.
Claim 7
Independent Claim
128 words
A method for producing a material useful in immunoassay comprising: (a) providing a disc shaped polymeric matrix which has groups reactive with proteins grafted onto its opposed surfaces; (b) distorting the opposed surfaces on said reactive groups with a designed configuration in the form of a plurality of ridges and depressions without puncturing the disc so that when said matrix is placed in the bottom of a vial both surfaces will be substantially in complete contact with any solution in the vial and there will be a minimum of surface-to-surface contact between the matrix and the bottom of the vial; (c) covalently bonding a protein onto the reactive groups to enable the material to be utilized in assaying the presence of material which react with the bound protein.
Claim 8
Independent Claim
128 words
A method for producing a material useful in immunoassay comprising: (a) providing a disc shaped polymeric matrix which has groups reactive with proteins grafted onto its opposed surfaces; (b) distorting the opposed surfaces on said reactive groups with a designed configuration in the form of a plurality of ridges and depressions without puncturing the disc so that when said matrix is placed in the bottom of a vial both surfaces will be substantially in complete contact with any solution in the vial and there will be a minimum of surface-to-surface contact between the matrix and the bottom of the vial; (c) covalently bonding a protein onto the reactive groups to enable the material to be utilized in assaying the presence of material which react with the bound protein.
Claim 9
Dependent Claim
31 words
The method as set forth in claim 6 wherein in step (c) an antibody reactive with antigens associated with hepatitis is covalently bonded to the reactive groups on said polymeric matrix.
Claim 10
Dependent Claim
21 words
The method as set forth in claim 6 wherein in step (b) said surfaces are distorted to have a waffle-like configuration.
Claim 11
Dependent Claim
32 words
The method as set forth in claim 8 wherein said waffle-like configuration is formed by passing the matrix between the surfaces of two rollers to press the configuration into the polymeric matrix.
Claim 12
Dependent Claim
31 words
The method as set forth in claim 9 wherein in step (c) an antibody reactive with antigens associated with hepatitis is covalently bonded to the reactive groups on said polymeric matrix.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-03-01 | 1985-08-31 | 1986-03-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-03-01 | 1989-08-31 | 1990-03-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-03-01 | 1993-08-31 | 1994-03-02 | $7700.00 | Window Closed / Lapsed |