US4828976A
Expired / Lapsed
US
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Glucose free media for storing blood platelets
Assignee: Thomas Jefferson University
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Filing: 1986-09-09
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Granted: 1989-05-09
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Expires: 2006-09-09
Abstract
Abstract Novel methods and storage media for storing platelets in a viable condition for at least five days are disclosed. The disclosed methods comprise the steps of providing a platelet rich suspension of platelets and blood plasma; extracting supernatant plasma from that suspension to leave between about 1 to 15 mls of plasma per unit of blood platelets with those platelets to produce a concentrated platelet button; adding 40 to 70 ml/unit of a glucose free aqueous solution to said concentrated platelet button; agitating the resultant solution to resuspend the platelets to provide a synthetic suspension of those platelets; and storing that synthetic suspension in an oxygen permeable container at about 22 DEG C., until needed for use. Two preferred glucose free aqueous platelet storage media are disclosed which generally comprise sodium citrate, sodium chloride, potassium chloride, and either sodium phosphate or calcium chloride, but not both. The subject media also optionally comprise magnesium sulfate. Results comparable to storing blood platelets in plasma are disclosed using media which free plasma for other uses and which may reduce the risk of disease transmission from platelet infusion.
Inventors
- 👤 Scott Murphy
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 05:03
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/19
Claims Analysis
Total Claims: 47
Claim 1
Independent Claim
93 words
An aqueous synthetic human blood platelet storage medium consisting essentially of: 75-125 mM sodium chloride; 17-29 mM sodium citrate; 3-7 mM potassium chloride; and an amount of phosphate buffer which is effective to maintain a unit of platelets and not more than 15 ml of associated plasma at a pH in excess of 6.6 during seven days of storage with 55 ml per unit of said medium in an oxygen permeable container maintained at from about 20° to 24° C., said synthetic blood platelet storage medium being essentially free of glucose and calcium.
Claim 2
Dependent Claim
93 words
An aqueous synthetic human blood platelet storage medium consisting essentially of: 75-125 mM sodium chloride; 17-29 mM sodium citrate; 3-7 mM potassium chloride; and an amount of phosphate buffer which is effective to maintain a unit of platelets and not more than 15 ml of associated plasma at a pH in excess of 6.6 during seven days of storage with 55 ml per unit of said medium in an oxygen permeable container maintained at from about 20° to 24° C., said synthetic blood platelet storage medium being essentially free of glucose and calcium.
Claim 3
Dependent Claim
12 words
The storage medium of claim 1 further comprising: 0.1-1.5 mM magnesium sulfate.
Claim 4
Dependent Claim
18 words
The medium of claim 11 wherein said amount of buffer is 19-31 mM NaH 2 PO 4 .
Claim 5
Dependent Claim
15 words
The storage medium of claim 1 wherein the concentration of sodium chloride is 90-110 mM.
Claim 6
Dependent Claim
15 words
The storage medium of claim 4 wherein said sodium chloride concentration is about 98 mM.
Claim 7
Dependent Claim
14 words
The storage medium of claim 1 wherein the sodium citrate concentration is 20-25 mM.
Claim 8
Dependent Claim
16 words
The storage medium of claim 6 wherein the concentration of sodium citrate is about 23 mM.
Claim 9
Dependent Claim
14 words
The storage medium of claim 1 wherein the potassium chloride concentration is 4-6 mM.
Claim 10
Dependent Claim
16 words
The storage medium of claim 8 wherein the concentration of potassium chloride is about 5 mM.
Claim 11
Dependent Claim
17 words
The storage medium of claim 8 wherein the concentration of NaH 2 PO 4 is 22-28 mM.
Claim 12
Dependent Claim
18 words
The storage medium of claim 10 wherein the concentration of NaH 2 PO 4 is about 25 mM.
Claim 13
Dependent Claim
27 words
An aqueous synthetic human blood platelet storage medium consisting essentially of: 70-120 mM sodium chloride; 17-29 mM sodium citrate; 3-7 mM potassium chloride; and 1.9-3.1 calcium chloride.
Claim 14
Dependent Claim
27 words
An aqueous synthetic human blood platelet storage medium consisting essentially of: 70-120 mM sodium chloride; 17-29 mM sodium citrate; 3-7 mM potassium chloride; and 1.9-3.1 calcium chloride.
Claim 15
Dependent Claim
88 words
An aqueous synthetic human blood platelet storage medium consisting essentially of: 100-300 mEq/L sodium; 50-300 mEq/L chloride; 0.1-10 mEq/L potassium; and an amount of phosphate which is effective to maintain the pH of a unit of platelets and not more than 15 ml of associated plasma at a pH in excess of 6.6 during seven days of storage with 55 ml/unit of said medium in an oxygen permeable container maintained at from 20° to 24° C., said blood platelet storage medium being essentially free of glucose and calcium.
Claim 16
Dependent Claim
88 words
An aqueous synthetic human blood platelet storage medium consisting essentially of: 100-300 mEq/L sodium; 50-300 mEq/L chloride; 0.1-10 mEq/L potassium; and an amount of phosphate which is effective to maintain the pH of a unit of platelets and not more than 15 ml of associated plasma at a pH in excess of 6.6 during seven days of storage with 55 ml/unit of said medium in an oxygen permeable container maintained at from 20° to 24° C., said blood platelet storage medium being essentially free of glucose and calcium.
Claim 17
Dependent Claim
15 words
The storage medium of claim 13 wherein said medium further comprises: 0.1-100 mEq/L of citrate.
Claim 18
Dependent Claim
14 words
The storage medium of claim 13 wherein the concentration of sodium is 140-240 mEq/L.
Claim 19
Dependent Claim
13 words
The medium of claim 15 wherein the concentration of sodium is 160-200 mEq/L.
Claim 20
Dependent Claim
13 words
The medium of claim 13 wherein the concentration of chloride is 80-150 mEq/L.
Claim 21
Dependent Claim
13 words
The medium of claim 17 wherein the concentration of chloride is 100-125 mEq/L.
Claim 22
Dependent Claim
14 words
The storage medium of claim 15 wherein the concentration of citrate is 45-75 mEq/L.
Claim 23
Dependent Claim
14 words
The storage medium of claim 19 wherein the concentration of citrate is 30-70 mEq/L.
Claim 24
Dependent Claim
14 words
The storage medium of claim 13 wherein the concentration of potassium is 2-7 mEq/L.
Claim 25
Dependent Claim
15 words
The storage medium of claim 21 wherein the concentration of potassium is about 5 mEq/L.
Claim 26
Dependent Claim
14 words
The storage medium of claim 14 wherein the concentration of magnesium is 1-4 mEq/L.
Claim 27
Dependent Claim
15 words
The storage medium of claim 23 wherein the concentration of magnesium is about 2.4 mEq/L.
Claim 28
Dependent Claim
13 words
The medium of claim 13 wherein said amount of phosphate is 5-50 mEq/L.
Claim 29
Dependent Claim
13 words
The medium of claim 25 wherein said amount of phosphate is 10-35 mEq/L.
Claim 30
Dependent Claim
14 words
The medium of claim 26 wherein said amount of phosphate is about 25 mEq/L.
Claim 31
Dependent Claim
69 words
An aqueous synthetic human blood platelet storage medium consisting essentially of: 100-300 mEq/L of sodium; 50-330 mEq/L of chloride; 0.1-10 mEq/L of calcium; and an amount of citrate sufficient to prevent clumping of platelets during resuspension of a unit of platelets in not more then 15 ml of associated plasma when resuspended in about 55 ml/unit of said medium, said storage medium being essentially free of glucose and phosphate.
Claim 32
Dependent Claim
69 words
An aqueous synthetic human blood platelet storage medium consisting essentially of: 100-300 mEq/L of sodium; 50-330 mEq/L of chloride; 0.1-10 mEq/L of calcium; and an amount of citrate sufficient to prevent clumping of platelets during resuspension of a unit of platelets in not more then 15 ml of associated plasma when resuspended in about 55 ml/unit of said medium, said storage medium being essentially free of glucose and phosphate.
Claim 33
Dependent Claim
19 words
The invention of claim 28 wherein said amount of calcium is an amount within the range of 0.1-10 mEq/L.
Claim 34
Dependent Claim
14 words
The storage medium of claim 29 wherein the concentration of calcium is 2-7 mEq/L.
Claim 35
Dependent Claim
15 words
The storage medium of claim 30 wherein the concentration of calcium is about 5 mEq/L.
Claim 36
Dependent Claim
12 words
The storage medium of claim 28 further comprising: 0.1-6 mEq/L of magnesium;
Claim 37
Dependent Claim
11 words
The storage medium of claim 32 comprising: 1-4 mEq/L of magnesium.
Claim 38
Dependent Claim
12 words
The storage medium of claim 33 comprising about 2.4 mEq/L of magnesium.
Claim 39
Dependent Claim
13 words
The invention of claim 28 wherein the concentration of citrate is 0.1-100 mEq/L.
Claim 40
Dependent Claim
13 words
The medium of claim 28 wherein the concentration of citrate is 45-75 mEq/L.
Claim 41
Dependent Claim
13 words
The medium of claim 36 wherein the concentration of citrate is 30-70 mEq/L.
Claim 42
Dependent Claim
14 words
The storage medium of claim 28 wherein the concentration of sodium is 140-240 mEq/L.
Claim 43
Dependent Claim
13 words
The medium of claim 38 wherein the concentration of sodium is 160-200 mEq/L.
Claim 44
Dependent Claim
13 words
The medium of claim 28 wherein the concentration of chloride is 80-150 mEq/L.
Claim 45
Dependent Claim
13 words
The medium of claim 40 wherein the concentration of chloride is 100-125 mEq/L.
Claim 46
Dependent Claim
16 words
The storage medium of claim 13 further comprising: a platelet permeable, non-glycolytic, substrate for oxidative phosphorylation.
Claim 47
Dependent Claim
16 words
The storage medium of claim 28 furthr comprising: a platelet permeable, non-glycolytic, substrate for oxidative phosphorylation.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-05-08 | 1992-11-07 | 1993-05-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-05-08 | 1996-11-07 | 1997-05-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-05-08 | 2000-11-07 | 2001-05-09 | $7700.00 | Window Closed / Lapsed |