US4814098A
Expired / Lapsed
US
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Magnetic material-physiologically active substance conjugate
Assignee: BELLEX SINGAPORE Corp
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Filing: 1987-08-28
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Granted: 1989-03-21
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Expires: 2007-08-28
Abstract
Abstract Disclosed is a conjugate comprising a magnetic material and a physiologically active substance bound to each other through a polyethylene glycol derivative, and a conjugate comprising a magnetic material and a polyethylene glycol derivative bound to each other. According to the present invention, a bioreactor enabling application to or recovery of physiologically active substances under liquid state by utilizing the magnetic properties is provided.
Inventors
- 👤 Yuji Inada
- 👤 Yutaka Tamaura
- 👤 Katsunobu Takahashi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 18:22
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54353; C; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/089; C12N11/14; C12N9/00; C12N9/14; C12N9/78; C12N9/80; C12N9/82; C12P; C12P1/00; G01N33/54313; G01N33/54326; G01N33/5434; G01N2446/00; G01N2446/20; G01N2446/30; G01N2446/80; G01N2446/86
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
20 words
A conjugate, comprising a magnetic material and a physiologically active substance bound to each other through a polyethyelen glycol derivative.
Claim 2
Dependent Claim
20 words
A conjugate, comprising a magnetic material and a physiologically active substance bound to each other through a polyethyelen glycol derivative.
Claim 3
Dependent Claim
15 words
A conjugate, comprising a magnetic material and a polyethylene glycol derivative bound to each other.
Claim 4
Dependent Claim
15 words
A conjugate, comprising a magnetic material and a polyethylene glycol derivative bound to each other.
Claim 5
Dependent Claim
18 words
The conjugate of claim 2, wherein the polyethylene glycol derivative has a molecular weight of 500 to 200,00.
Claim 6
Dependent Claim
40 words
The conjugate according to claim 2, wherein the polyethylene glycol derivative is α, ω-di-carboxy polyethylene glycol or α,ω-dimethozycarbonyl polyethylene glycol having a molecular weight of 500 to 200,00 and the magnetic material is formed from ferrous chloride and ferric chloride.
Claim 7
Dependent Claim
13 words
The conjugate according to claim 4, further comprising enzyme bound to the conjugate.
Claim 8
Dependent Claim
19 words
The conjugate according to claim 1, wherein the polyethylene glycol derivative has a molecular weight of 500 to 200,000.
Claim 9
Dependent Claim
37 words
The conjugate according to claim 1, wherein the polyethylene glycol derivative has at least one selected from the group consisiting of hydroxyl group, carboxyl group, methoxycarbonyl group, amide group, amino group and mthoxy group at termainal ends.
Claim 10
Dependent Claim
20 words
The conjugate according to claim 1, wherein the polyethylene glycol derivative has carboxyl group or methoxycarbonyl group at terminal ends.
Claim 11
Dependent Claim
27 words
The conjugate according to claim 1, wherein the magnetic material is a transition metal or its ion, its oxide or a compound of these with other elements.
Claim 12
Dependent Claim
18 words
The conjugate according to claim 1, wherein the magnetic material is formed from ferrous chloride or ferric chloride.
Claim 13
Dependent Claim
14 words
The conjugate according to claim 1, wherein the magnetic material is ferrite or magnetite.
Claim 14
Dependent Claim
16 words
The conjugate according to claim 1, wherein the magnetic material is erbium oxide or dysprosium chloride.
Claim 15
Dependent Claim
60 words
The conjugate according to claim 1, wherein the physiologically active aubstance is an enzyme, a protein, an antibody, an antigen, a polysaccharide, a nucleic acid, a lipid, an amino acid, a co-enzyme, ATP, ADP, heme, riboflavin, a hormone, a vitamin, a ligand, an antibiotic, an antitumor substance, or a chloroplast, a mitochrondrion, a virus, or a protein constituting Bacterial cell.
Claim 16
Dependent Claim
37 words
The conjugate of claim 13, wherein the polyethylene glycol derivative has at least one group selected from the group consisting of hydroxyl group, carboxyl group, methoxycarbonyl group, amide group, amino group and methoxy group, at terminal ends.
Claim 17
Dependent Claim
20 words
The conjugate according to claim 14, wherein the polyethylene glycol derivative has carobyxl group or methoxycarbonyl group at terminal ends.
Claim 18
Dependent Claim
27 words
The conjugate according to claim 14, wherein the magnetic material is a transition metal or its ion, its oxide or a compound of these with other elements.
Claim 19
Dependent Claim
18 words
The conjugate according to claim 14, wherein the magnetic material is formed form ferrous chloride or ferric chloride.
Claim 20
Dependent Claim
14 words
The conjugate according to claim 14, wherein the magnetic material is ferrite or magnetite.
Claim 21
Dependent Claim
16 words
The conjugate according to claim 14, wherein the magnetic material is erbium oxide or dysprosium chloride.
Claim 22
Dependent Claim
18 words
The conjugate of claim 14, wherein the polyethylene glycol derivative has a molecular weight of 500 to 200,000.
Claim 23
Dependent Claim
21 words
The conjugate according to claim 1, wherein the physiologically active substance is an enzyme, a protein, an antibody or an antigen.
Claim 24
Dependent Claim
14 words
The conjugate according to claim 1, wherein the physiologically active substance is an enzyme.
Claim 25
Dependent Claim
13 words
The conjugate according to claim 1, wherein the physiologically active substance is lipase.
Claim 26
Dependent Claim
18 words
The conjugate of claim 14 wherein the polyethylene glycol derivative has a molecular weight of 500 to 200,000.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-03-20 | 1992-09-19 | 1993-03-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-03-20 | 1996-09-19 | 1997-03-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-03-20 | 2000-09-19 | 2001-03-21 | $7700.00 | Window Closed / Lapsed |