US4645741A
Expired / Lapsed
US
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Modified lipase
Assignee: TOINGAKUEN SCHOOL SYSTEM
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Filing: 1984-12-31
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Granted: 1987-02-24
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Expires: 2004-12-31
Abstract
Abstract A modified lipase, comprising lipase molecules which are partially substituted with an active derivative of a polyalkylene glcyol having a hydrophobic group at the terminal end.
Inventors
- 👤 Yuji Inada
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 04:21
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/62; C07; C07J; C07J9/00; C12N; C12N9/00; C12N9/14; C12N9/16; C12N9/18; C12N9/20; C12N9/96
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
26 words
A modified lipase, comprising lipase molecules which are partially substituted with an active derivative of a polyalkylene glycol having a hydrophobic group at the terminal end.
Claim 2
Dependent Claim
26 words
A modified lipase, comprising lipase molecules which are partially substituted with an active derivative of a polyalkylene glycol having a hydrophobic group at the terminal end.
Claim 3
Dependent Claim
23 words
The modified lipase according to claim 1, wherein the polyalkylene glycol is a polyethylene glycol having a molecular weight of 5,000 or more.
Claim 4
Dependent Claim
23 words
The modified lipase according to claim 1, wherein the hydrophobic group at the terminal end is an alkyl group or an aryl group.
Claim 5
Dependent Claim
29 words
The modified lipase according to claim 1, wherein the active derivative is a triazine derivative, an acetyl derivative, a diazonium derivative, an amino derivative or a carboxylic acid derivative.
Claim 6
Dependent Claim
40 words
The modified lipase according to claim 1, wherein the amino groups in the lipase molecules are partially substituted with a group of the formula: ##STR8## wherein R represents a polyalkylene glycol group having a hydrophobic group at the terminal end.
Claim 7
Dependent Claim
26 words
The modified lipase according to claim 5, which is substituted with a 2,4-bis(methoxypolyoxyethylene)-6-triazine of which the polyoxyethylene moiety has a molecular weight of 5,000 or more.
Claim 8
Dependent Claim
74 words
A process for producing an ester, which comprises effecting in an organic solvent (i) esterification by allowing a carboxylic acid to react with an alcohol or (ii) interesterification by allowing an ester of carboxylic acid to react with other carboxylic acid or an alcohol, in the presence of a modified lipase obtained by allowing an active derivative of a polyalkylene glycol having a hydrophobic group at the terminal end to react with a lipase.
Claim 9
Dependent Claim
74 words
A process for producing an ester, which comprises effecting in an organic solvent (i) esterification by allowing a carboxylic acid to react with an alcohol or (ii) interesterification by allowing an ester of carboxylic acid to react with other carboxylic acid or an alcohol, in the presence of a modified lipase obtained by allowing an active derivative of a polyalkylene glycol having a hydrophobic group at the terminal end to react with a lipase.
Claim 10
Dependent Claim
21 words
The process of claim 1, wherein the polyalkylene glycol is a polyethylene glycol having a molecular weight of 5,000 or more.
Claim 11
Dependent Claim
21 words
The process of claim 1, wherein the hydrophobic group at the terminal end is an alkyl group or an aryl group.
Claim 12
Dependent Claim
27 words
The process of claim 1, wherein the active derivative is a triazine derivative, an acetyl derivative, a diazonium derivative, an amino derivative or a carboxylic acid derivative.
Claim 13
Dependent Claim
37 words
The process of claim 1 wherein lipase molecules contain amino groups which are partially substituted with a group of the formula: ##STR9## wherein R represents a polyalkylene glycol group having a hydrophobic group at the terminal end.
Claim 14
Dependent Claim
27 words
The process of claim 11, wherein said modified lipase is substituted with a 2,4-bis(methoxyoxyethylene)-6-triazine of which the polyoxyethylene moiety has a molecular weight of 5,000 or more.
Claim 15
Dependent Claim
64 words
A modified lipase, comprising lipase molecules which are partially substituted with an active derivative of a polyalkylene glycol having a hydrophobic group at the terminal end which promotes (i) esterification by allowing a carboxylic acid to react with an alcohol or (ii) interesterification by allowing an ester of a carboxylic acid to react with other carboxylic acid or an alcohol, in an organic solvent.
Claim 16
Dependent Claim
64 words
A modified lipase, comprising lipase molecules which are partially substituted with an active derivative of a polyalkylene glycol having a hydrophobic group at the terminal end which promotes (i) esterification by allowing a carboxylic acid to react with an alcohol or (ii) interesterification by allowing an ester of a carboxylic acid to react with other carboxylic acid or an alcohol, in an organic solvent.
References Cited (Backward Citations)
US4179337A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-02-23 | 1990-08-25 | 1991-02-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-02-23 | 1994-08-25 | 1995-02-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-02-23 | 1998-08-25 | 1999-02-24 | $7700.00 | Window Closed / Lapsed |