US4810642A
Expired / Lapsed
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Method and test composition for determination of hydrogen peroxide
Assignee: Minaris Medical Co Ltd
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Filing: 1986-06-25
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Granted: 1989-03-07
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Expires: 2006-06-25
Abstract
Abstract A method and test composition for colorimetrically determining hydrogen peroxide in a sample includes a chromogen of the formula: <IMAGE> which is reacted with hydrogen peroxide in the sample in the presence of peroxidase. The absorbancy of the reaction solution is then measured.
Inventors
- 👤 Norihito Aoyama
- 👤 Mikio Okano
- 👤 Akira Miike
- 👤 Toshio Tatano
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 20:35
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12Q2326/00; C12Q2326/40; Y; Y10; Y10S; Y10S436/00; Y10S436/904; Y10T; Y10T436/00; Y10T436/20; Y10T436/206664
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
176 words
A method for the determination of hydrogen peroxide which comprises reacting a chromogen represented by the general formula (I) ##STR5## wherein Y is hydrogen or hydroxyl; R 1 is a group represented by the general formula (II) or (III) ##STR6## wherein Z is hydroxyl, alkyl or carboxyl; s, t, u and w is 0 or an integer of 1 to 3; and each Z in Z s , each Z in Z t , each Z in Z u and each Z in Z w are the same or different, P and Q are hydroxyl, alkyl, sulfo or carboxyl; n and m is 0 or an integer of 1 to 3; each P in P n and each Q in Q m are the same or different and R 2 and R 3 may be the same or different and are hydroxyl, amino or substituted amino or salt thereof, with hydrogen peroxide in the presence of peroxidase; and measuring the absorbancy of the reaction solution colored by formation of a pigment in the visible ray region.
Claim 2
Independent Claim
176 words
A method for the determination of hydrogen peroxide which comprises reacting a chromogen represented by the general formula (I) ##STR5## wherein Y is hydrogen or hydroxyl; R 1 is a group represented by the general formula (II) or (III) ##STR6## wherein Z is hydroxyl, alkyl or carboxyl; s, t, u and w is 0 or an integer of 1 to 3; and each Z in Z s , each Z in Z t , each Z in Z u and each Z in Z w are the same or different, P and Q are hydroxyl, alkyl, sulfo or carboxyl; n and m is 0 or an integer of 1 to 3; each P in P n and each Q in Q m are the same or different and R 2 and R 3 may be the same or different and are hydroxyl, amino or substituted amino or salt thereof, with hydrogen peroxide in the presence of peroxidase; and measuring the absorbancy of the reaction solution colored by formation of a pigment in the visible ray region.
Claim 3
Dependent Claim
16 words
A method according to claim 1, wherein said reaction is carried out in an alkaline solution.
Claim 4
Dependent Claim
16 words
A method according to claim 1, wherein said R 2 and R 3 are dimethylamino group.
Claim 5
Dependent Claim
16 words
A method according to claim 1, wherein said reaction is carried out in a buffer solution.
Claim 6
Dependent Claim
24 words
A method according to claim 4, wherein said buffer is selected from the group consisting of phosphate, Tris-hydrochloride, succinate, oxalate, citrate, acetate and Good's.
Claim 7
Dependent Claim
17 words
A method according to claim 1, wherein said reaction is carried out in the presence of surfactant.
Claim 8
Dependent Claim
14 words
A method according to claim 6, wherein said surfactant is polyethylene gylcol mono-p-iso-octylphenyl ether.
Claim 9
Dependent Claim
17 words
A method according to claim 1, wherein said hydrogen peroxide is a product formed by enzymatic reaction.
Claim 10
Dependent Claim
23 words
A method according to claim 8, wherein said hydrogen peroxide-producing reaction and the reaction of hydrogen peroxide with said chromogen are conducted simultaneously.
Claim 11
Dependent Claim
18 words
A method according to claim 8, wherein said enzymatic reaction is the oxidation of a substrate using oxidase.
Claim 12
Dependent Claim
43 words
A method according to claim 10, wherein said oxidase is selected from the group consisting of uricase, cholesterol oxidase, xanthin oxidase, choline oxidase, pyruvate oxidase, glicerin-3-phosphate oxidase, acyl CoA oxidase, glycerol oxidase, glucose oxidase, pyranose oxidase, sarcosine oxidase, putrescine oxidase and galactose oxidase.
Claim 13
Dependent Claim
19 words
A test composition for the determination of hydrogen peroxide which comprises the chromogen defined in claim 1 and peroxidase.
Claim 14
Dependent Claim
15 words
A test composition according to claim 12, wherein said composition further contains a buffer reagent.
Claim 15
Dependent Claim
26 words
A test composition according to claim 12, wherein said composition further contains a member selected from the group consisting of a surfactant, antiseptics and ascorbate oxidase.
Claim 16
Dependent Claim
17 words
A test composition according to claim 12, wherein said composition further contains an enzymatic hydrogen peroxide-producing system.
Claim 17
Dependent Claim
12 words
A test composition according to claim 12, wherein said system contains oxidase.
References Cited (Backward Citations)
EP0024578A1
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EP0029104A1
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EP0057661A2
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US4587100A
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EP0108526A2
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EP0120399A2
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EP0124287A2
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EP0121254A2
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EP0124909A2
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EP0153872A2
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1992-03-06 | 1992-09-05 | 1993-03-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-03-06 | 1996-09-05 | 1997-03-07 | $3760.00 | Window Closed / Lapsed |
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