Method and test composition for determination of hydrogen peroxide

Claim 1

A method for the determination of hydrogen peroxide in a sample which comprises reacting the hydrogen peroxide with a peroxidase in the presence of a chromogen and measuring the absorbancy of the reaction solution in the visible or near inflared ray region characterise in that the chromogen is a compound of the formula or wherein Y represents hydrogen or Z represents an oxygen atom or a sulfur atom, X represents hydrogen, alkyl, alkenyl, aryl, aralkyl, amino or substituted amino, R 1 represents hydroxyl, amino or substituted amino, R 2 represents hydrogen, hydroxyl, alkyl, alkoxy, aryl, aralkyl, alkenyl, amino or substituted amino, R 3 represents groups represented by the general formula (III), (IV), (V), (VI) or (VII) described hereinafter, R 4 , R 5 or R 6 .may be the same or different and represents hydrogen, alkyl, alkenyl, acyl, aryl, aralkyl, halogen atom, nitro, sulfo, carboxyl, hydroxyl, alkoxy or groups represented by the general formula (III), (IV), (V), (VI) or (VII), J represents -S-, -O-, the general formula or the general formula R 7 and R 8 are the same or different and represent hydrogen atom, alkyl or alkenyl, and - R 5 and R 6 may form alkenylene , the said general formulae III to VII being as follows: General formula (III) General formula (IV) General formula (V) General formula (VI). General formula (VII) wherein A 1 represents alkylene, A 2 has the same meaning as that of R 2 , B 1 , B 2 , B 3 , B 4 , B 5 or B 6 may. be the same or different and represents hydrogen atom, alkyl, alkenyl, acyl, aryl, aralkyl, halogen atom, nitro f sulfo, carboxyl, hydroxyl, alkoxy or hydroxyalkyl.

Claim 2

A method for the determination of hydrogen peroxide in a sample which comprises reacting the hydrogen peroxide with a peroxidase in the presence of a chromogen and measuring the absorbancy of the reaction solution in the visible or near inflared ray region characterise in that the chromogen is a compound of the formula or wherein Y represents hydrogen or Z represents an oxygen atom or a sulfur atom, X represents hydrogen, alkyl, alkenyl, aryl, aralkyl, amino or substituted amino, R 1 represents hydroxyl, amino or substituted amino, R 2 represents hydrogen, hydroxyl, alkyl, alkoxy, aryl, aralkyl, alkenyl, amino or substituted amino, R 3 represents groups represented by the general formula (III), (IV), (V), (VI) or (VII) described hereinafter, R 4 , R 5 or R 6 .may be the same or different and represents hydrogen, alkyl, alkenyl, acyl, aryl, aralkyl, halogen atom, nitro, sulfo, carboxyl, hydroxyl, alkoxy or groups represented by the general formula (III), (IV), (V), (VI) or (VII), J represents -S-, -O-, the general formula or the general formula R 7 and R 8 are the same or different and represent hydrogen atom, alkyl or alkenyl, and - R 5 and R 6 may form alkenylene , the said general formulae III to VII being as follows: General formula (III) General formula (IV) General formula (V) General formula (VI). General formula (VII) wherein A 1 represents alkylene, A 2 has the same meaning as that of R 2 , B 1 , B 2 , B 3 , B 4 , B 5 or B 6 may. be the same or different and represents hydrogen atom, alkyl, alkenyl, acyl, aryl, aralkyl, halogen atom, nitro f sulfo, carboxyl, hydroxyl, alkoxy or hydroxyalkyl.