EP0124909A2
Expired / Lapsed
EP
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Process for determining reduced form coenzymes
Assignee: Fujifilm Wako Pure Chemical Corp
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Filing: 1984-05-09
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Granted: —
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Expires: 2004-05-09
Abstract
Abstract A reduced form coenzyme can be determined by acting a peroxidase on a reduced form coenzyme in the presence of metal ions to generate hydrogen peroxide and measuring the amount of hydrogen peroxide colorimetrically.
Inventors
- 👤 Kazuhiko Yamanishi
- 👤 Toshiro Hanada
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 10:43
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12Q1/008; C12Q1/60
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
36 words
A process for determining a reduced form coenzyme which comprises acting a peroxidase on a reduced form coenzyme in the presence of metal ions to generate hydrogen peroxide, and measuring the amount of hydrogen eproxide colorimetrically.
Claim 2
Dependent Claim
36 words
A process for determining a reduced form coenzyme which comprises acting a peroxidase on a reduced form coenzyme in the presence of metal ions to generate hydrogen peroxide, and measuring the amount of hydrogen eproxide colorimetrically.
Claim 3
Dependent Claim
23 words
A process according to Claim 1, wherein the reduced form coenzyme is nicotinamide adenine dinucleotide, reduced form or nicotinamide adenine dinucleotidephosphate, reduced form.
Claim 4
Dependent Claim
18 words
A process according to Claim 1, wherein the metal ions are divalent manganese ions and/or divalent cobalt ions.
Claim 5
Dependent Claim
26 words
A process according to Claim 1, wherein the reduced form coenzyme is obtained by acting a dehydrogenase on a substrate in the presence of a coenzyme.
Claim 6
Dependent Claim
28 words
A process according to Claim 4, wherein the substrate is lactic acid, the dehydrogenase is lactate dehydrogenase, and the coenzyme is nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotidephosphate.
Claim 7
Dependent Claim
28 words
A process according to Claim 4, wherein the substrate is a-hydroxybutyric acid, the dehydrogenase is a-hydroxybutyrate dehydrogenase, and the coenzyme is nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotidephosphate.
Claim 8
Dependent Claim
27 words
A process according to Claim 4, wherein the substrate is cholesterol, the dehydrogenase is cholesterol dehydrogenase, and the coenzyme is nicotinamid adenine dinucleotide or nicotinamide adenine dinucleotidephosphate.
Claim 9
Dependent Claim
28 words
A process according to Claim 4, wherein the substrate is bile acids, the dehydrogenase is 3a-hydroxysteroid dehydrogenase, and the coenzyme is nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotidephosphate.
Claim 10
Dependent Claim
32 words
A process according to Claim 4, wherein the substrate is glycerol or aldehyde, the dehydrogenase is glycerol dehydrogenase or aldehyde dehydrogenase, and the coenzyme is nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotidephosphate.
Claim 11
Dependent Claim
32 words
A process according to Claim 4, wherein the substrate is glycerol-3-phosphate or glucose-6-phosphate, the dehydrogenase is glycerol-3-phosphate dehydrogenase or glucose-6-phosphate dehydrogenase, and the coenzyme is nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotidephosphate.
References Cited (Backward Citations)
No citation records indexed.
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