Patent US4804625A successfully refreshed from Google Patents.
US4804625A
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US
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Assay procedures
Assignee: BP Corp North America Inc
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Filing: 1984-09-27
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Granted: 1989-02-14
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Expires: 2004-09-27
Abstract
Abstract Binding assay methods involving determining the presence of analytes in samples through enzymatic formation of detectable substances in amounts related to the amount of analyte present in the sample and monitoring for the presence of the substances in distinct phases. Methods according to the invention include use of labelled materials which associate with the analyte to be determined or compete with the analyte for association with an added binder. The labelled materials employed include label portions which enzymatically form substances from substrates provided in or existing as a first phase, or, upon enzymatic treatment in a first phase, disassociate into substances capable of existing in or as a second distinct phase. Formation of the detectable substances is monitored by determining the transfer of the substance to a second distinct phase in contact with the first phase or by determining formation of a second distinct phase. The assays are useful in determining human IgG protein in blood samples and other constituents of blood or other biological samples without elaborate instrumentation, allowing for practice outside the clinical laboratory.
Inventors
- 👤 Larry E. Morrison
- 👤 Garfield P. Royer
- 👤 Michael J. Heller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 19:09
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S436/00; Y10S436/805
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
186 words
A method for determining the presence of an analyte in a sample comprising contacting a sample with a reagent adapted to form a substrate phase and a product phase with said sample, said reagent including an enzyme-substrate pair, including an enzyme and a substrate, and labeled material, capable of assuming a bound position with said analyte, said labeled material having a label moiety capable of participating in reactions related to the presence of said analyte which reactions require said substrate present in said substrate phase and produce a reaction product, said label moiety is a member of said enzyme-substrate pair, said enzyme catalyzing a reaction with said substrate which produces said reaction product, said enzyme-substrate pair is selected from the group of enzyme-substrate pairs consisting of alkaline phosphatase and the substrate p-phenylazophenyl phosphate, and the enzyme collagenase and the substrate collagen/blue dextran, said reaction product capable of partitioning into said product phase distinct from said substrate phase wherein said substrate phase is a solid or a liquid, and said product phase is a liquid; and monitoring said product phase for the presence of said reaction product.
Claim 2
Independent Claim
186 words
A method for determining the presence of an analyte in a sample comprising contacting a sample with a reagent adapted to form a substrate phase and a product phase with said sample, said reagent including an enzyme-substrate pair, including an enzyme and a substrate, and labeled material, capable of assuming a bound position with said analyte, said labeled material having a label moiety capable of participating in reactions related to the presence of said analyte which reactions require said substrate present in said substrate phase and produce a reaction product, said label moiety is a member of said enzyme-substrate pair, said enzyme catalyzing a reaction with said substrate which produces said reaction product, said enzyme-substrate pair is selected from the group of enzyme-substrate pairs consisting of alkaline phosphatase and the substrate p-phenylazophenyl phosphate, and the enzyme collagenase and the substrate collagen/blue dextran, said reaction product capable of partitioning into said product phase distinct from said substrate phase wherein said substrate phase is a solid or a liquid, and said product phase is a liquid; and monitoring said product phase for the presence of said reaction product.
Claim 3
Dependent Claim
18 words
The method of claim 1 wherein said analyte and labeled material are members of a biological binding pair.
Claim 4
Dependent Claim
35 words
The method of claim 2 wherein said biological binding pair is selected from the group of biological binding pairs consisting of antigens and antibodies, complementary polynucleotides, drugs and drug receptors, and enzyme and enzyme substrates.
Claim 5
Dependent Claim
186 words
A kit for determining the presence of an analyte in a sample comprising: reagent adapted to form a substrate phase and a product phase with said sample, said reagent including an enzyme-substrate pair, including an enzyme and a substrate, and labeled material, capable of assuming a bound position with said analyte, said labeled material having a label moiety capable of participating in reactions related to the presence of said analyte which reactions require a substrate present in said substrate phase and produce a reaction product, said label moiety is a member of said enzyme-substrate pair, said enzyme catalyzing a reaction with said substrate which produces said reaction product, said enzyme substrate pair is selected from the group of enzyme-substrate pairs consisting of the enzyme alkaline phosphatase and the substrate p-phenylazophenyl phosphate, the enzyme collagenase and the substrate collagen/blue dextran, said reaction product capable of partitioning into said product phase distinct from said substrate phase wherein said substrate phase is a solid or a liquid and said product phase is a liquid; said reaction product detectable within said product phase to indicate the presence of said analyte.
Claim 6
Dependent Claim
186 words
A kit for determining the presence of an analyte in a sample comprising: reagent adapted to form a substrate phase and a product phase with said sample, said reagent including an enzyme-substrate pair, including an enzyme and a substrate, and labeled material, capable of assuming a bound position with said analyte, said labeled material having a label moiety capable of participating in reactions related to the presence of said analyte which reactions require a substrate present in said substrate phase and produce a reaction product, said label moiety is a member of said enzyme-substrate pair, said enzyme catalyzing a reaction with said substrate which produces said reaction product, said enzyme substrate pair is selected from the group of enzyme-substrate pairs consisting of the enzyme alkaline phosphatase and the substrate p-phenylazophenyl phosphate, the enzyme collagenase and the substrate collagen/blue dextran, said reaction product capable of partitioning into said product phase distinct from said substrate phase wherein said substrate phase is a solid or a liquid and said product phase is a liquid; said reaction product detectable within said product phase to indicate the presence of said analyte.
Claim 7
Dependent Claim
18 words
The kit of claim 4 wherein said analyte and labeled material are members of a biological binding pair.
Claim 8
Dependent Claim
35 words
The kit of claim 5 wherein said biological binding pair is selected from the group of biological binding pairs consisting of antigens and antibodies, complementary polynucleotides, drugs and drug receptors, and enzyme and enzyme substrates.
Claim 9
Independent Claim
141 words
A method for determining the presence of an analyte in a sample comprising contracting a sample with a reagent adapted to form a substrate phase and a product phase with said sample, said reagent including an enzyme-substrate pair, including an enzyme and a substrate, and labeled material, capable of assuming a bound position with said analyte, said labeled materials having a label moiety capable of participating in reactions related to the presence of said analyte which reactions require said substrate present in said substrate phase and produce a reaction product, said product capable of partitioning into said product phase distinct from said substrate phase wherein said substrate phase is a solid or a liquid, and said product phase is a gas and said product is detectable by olfactory means; and monitoring said product phase for the presence of said reaction product.
Claim 10
Independent Claim
141 words
A method for determining the presence of an analyte in a sample comprising contracting a sample with a reagent adapted to form a substrate phase and a product phase with said sample, said reagent including an enzyme-substrate pair, including an enzyme and a substrate, and labeled material, capable of assuming a bound position with said analyte, said labeled materials having a label moiety capable of participating in reactions related to the presence of said analyte which reactions require said substrate present in said substrate phase and produce a reaction product, said product capable of partitioning into said product phase distinct from said substrate phase wherein said substrate phase is a solid or a liquid, and said product phase is a gas and said product is detectable by olfactory means; and monitoring said product phase for the presence of said reaction product.
Claim 11
Dependent Claim
28 words
The method of claim 7 wherein said label moiety is a member of an enzyme-substrate pair, said enzyme catalyzing a reaction with said substrate which products said product.
Claim 12
Dependent Claim
18 words
The method of claim 8 wherein said enzyme substrate pair includes the enzyme peroxidase and the substrate 2-methyl-3-oxobutanal.
Claim 13
Dependent Claim
81 words
A kit for determining the presence of an analyte in a sample comprising: reagent including labeled material capable of assuming a bound position with said analyte, said labeled material having a label moiety capable of participating in reactions related to the presence of said analyte which produce a reaction product, and said label moiety is a member of an enzyme-substrate which includes the enzyme peroxidase and the substrate 2-methyl-3-oxobutanal, said reaction product being detectable to indicate the presence of said analyte.
Claim 14
Dependent Claim
81 words
A kit for determining the presence of an analyte in a sample comprising: reagent including labeled material capable of assuming a bound position with said analyte, said labeled material having a label moiety capable of participating in reactions related to the presence of said analyte which produce a reaction product, and said label moiety is a member of an enzyme-substrate which includes the enzyme peroxidase and the substrate 2-methyl-3-oxobutanal, said reaction product being detectable to indicate the presence of said analyte.
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