US4423143A
Expired / Lapsed
US
⏱️ 0.0 years remaining
β-D-Galactosidase conjugate for enzyme immunoassays
Assignee: Siemens Healthcare Diagnostics GmbH Germany
•
Filing: 1981-04-29
•
Granted: 1983-12-27
•
Expires: 2001-04-29
Abstract
Abstract beta -D-Galactosidase conjugates for use in homogeneous immunoassays. beta -D-Galactosidase is conjugated with analytes and the resulting conjugates are combined with receptors for the analyte and a sample suspected of containing the analyte. The resulting enzymatic activity is compared to standard assay media for quantitative determination of the analyte.
Inventors
- 👤 Kenneth E. Rubenstein
- 👤 Edwin F. Ullman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 19:10
CPC Classifications
C; C07; C07J; C07J41/00; C07J41/0005; C07J41/0016; C07J19/00; C07J43/00; C07J43/003; C12; C12N; C12N9/00; C12N9/96; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; G01N33/74; G01N33/743; G01N33/94; Y; Y10; Y10S; Y10S435/00; Y10S435/964
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
70 words
An enzyme conjugate of β-D-galactosidase bonded to on the average with from about 1 to 24 haptens of molecular weight of from about 125 to 1,000 and having at least one heteroatom, wherein said conjugated enzyme retains at least about 10% of the original enzyme activity and wherein the enzymatic activity of the conjugate is reduced by at least 30%, when said haptens are bound to receptors for said haptens.
Claim 2
Dependent Claim
70 words
An enzyme conjugate of β-D-galactosidase bonded to on the average with from about 1 to 24 haptens of molecular weight of from about 125 to 1,000 and having at least one heteroatom, wherein said conjugated enzyme retains at least about 10% of the original enzyme activity and wherein the enzymatic activity of the conjugate is reduced by at least 30%, when said haptens are bound to receptors for said haptens.
Claim 3
Dependent Claim
54 words
An enzyme conjugate of β-D-galactosidase of the formula (β-gal).sub.n -(X*-L*).sub.m wherein: β-gal intends β-D-galactosidase; X* is a bond or linking group; L* is a ligand of from about 125 to 1,000 molecular weight and having at least one heteroatom; n is 1; and m is in the range of 2 to 20 per subunit.
Claim 4
Dependent Claim
54 words
An enzyme conjugate of β-D-galactosidase of the formula (β-gal).sub.n -(X*-L*).sub.m wherein: β-gal intends β-D-galactosidase; X* is a bond or linking group; L* is a ligand of from about 125 to 1,000 molecular weight and having at least one heteroatom; n is 1; and m is in the range of 2 to 20 per subunit.
Claim 5
Dependent Claim
48 words
An enzyme conjugate of the formula: ##STR4## wherein: β-gal is β-D-galactosidase; X* is a bond or linking group; Z is hydrogen or a functionality or side chain associated with a steroid; any one of the ring carbons may be substituted; and m is 2 to 20 per subunit.
Claim 6
Dependent Claim
48 words
An enzyme conjugate of the formula: ##STR4## wherein: β-gal is β-D-galactosidase; X* is a bond or linking group; Z is hydrogen or a functionality or side chain associated with a steroid; any one of the ring carbons may be substituted; and m is 2 to 20 per subunit.
Claim 7
Dependent Claim
5 words
A β-D-galactosidase conjugate with digoxigenin.
Claim 8
Dependent Claim
5 words
A β-D-galactosidase conjugate with digoxigenin.
Claim 9
Independent Claim
91 words
A method for determining the presence of a ligand in a medium suspected of containing said ligand, which comprises bringing together in an aqueous liquid zone: (1) said medium; (2) soluble β-D-galactosidase-bound-ligand; and (3) soluble receptor having sites common to and capable of binding to said ligand and said enzyme-bound ligand; wherein said receptor is at a concentration resulting in substantial reduction in enzymatic activity of said β-D-galactosidase-bound-ligand in the absence of ligand; and analyzing in said zone for the effect of said medium on the enzymatic activity of said β-D-galactosidase-bound-ligand.
Claim 10
Independent Claim
91 words
A method for determining the presence of a ligand in a medium suspected of containing said ligand, which comprises bringing together in an aqueous liquid zone: (1) said medium; (2) soluble β-D-galactosidase-bound-ligand; and (3) soluble receptor having sites common to and capable of binding to said ligand and said enzyme-bound ligand; wherein said receptor is at a concentration resulting in substantial reduction in enzymatic activity of said β-D-galactosidase-bound-ligand in the absence of ligand; and analyzing in said zone for the effect of said medium on the enzymatic activity of said β-D-galactosidase-bound-ligand.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-12-26 | 1987-06-27 | 1987-12-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-12-26 | 1991-06-27 | 1991-12-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-12-26 | 1995-06-27 | 1995-12-27 | $7700.00 | Window Closed / Lapsed |