US4587212A
Expired / Lapsed
US
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Immunoassay
Assignee: Boots Celltech Diagnostics Ltd
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Filing: 1983-05-18
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Granted: 1986-05-06
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Expires: 2003-05-18
Abstract
Abstract There is described an enzyme immunoassay for measuring the concentration of an analyte in a sample wherein the substrate for the enzyme forms at least a part of the sample. In a particular embodiment the sample comprises or consists of a milk sample and the enzyme is an enzyme capable of clotting milk. An example given of such an enzyme is chymosin. The assay described may be used to measure the concentration of progestagens or oestrogens in milk using the techniques of heterogeneous or homogeneous enzyme immunoassay. The results of such an assay give an indication of the fertility of a milk producing domestic animal (e.g. a cow) and may be used to diagnose pregnancy of such an animal. Particular compounds for use in the assay are described, as is a kit of reagents for use in the assay.
Inventors
- 👤 Terence S. Baker
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 19:49
CPC Classifications
C; C07; C07J; C07J17/00; C07J17/005; C07J41/00; C07J41/0033; C07J41/0072; C07J43/00; C07J43/003; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/743; Y; Y10; Y10S; Y10S435/00; Y10S435/964; Y10S436/00; Y10S436/811; Y10S436/814; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
64 words
In an enzyme immunoassay for measuring the concentration of an antigenic analyte in a milk sample wherein the enzyme labeled binding partner is introduced to said milk sample and wherein enzyme activity provides a measure of analyte concentration, the improvement wherein the enzyme is an enzyme capable of clotting milk and the substrate for the enzyme forms at least part of the milk sample.
Claim 2
Dependent Claim
64 words
In an enzyme immunoassay for measuring the concentration of an antigenic analyte in a milk sample wherein the enzyme labeled binding partner is introduced to said milk sample and wherein enzyme activity provides a measure of analyte concentration, the improvement wherein the enzyme is an enzyme capable of clotting milk and the substrate for the enzyme forms at least part of the milk sample.
Claim 3
Dependent Claim
12 words
An enzyme immunoassay according to claim 1 wherein the enzyme is chymosin.
Claim 4
Dependent Claim
16 words
An enzyme immunoassay according to claim 1 wherein the enzyme immunoassay is a homogeneous enzyme immunoassay.
Claim 5
Dependent Claim
31 words
An enzyme immunoassay according to claim 1 wherein the antigenic analyte is a steroid selected from the group consisting of a progestagen, a progestagen metabolite, an oestrogen and an oestrogen metabolite.
Claim 6
Dependent Claim
13 words
An enzyme immunoassay according to claim 4 wherein the antigenic analyte is progesterone.
Claim 7
Dependent Claim
14 words
An enzyme immunoassay according to claim 4 wherein the antigenic analyte is oestrone sulphate.
Claim 8
Dependent Claim
50 words
A method for detecting the onset of oestrus in a domestic milk-producing animal comprising using an enzyme immunoassay according to claim 5 to monitor the concentration of a progesterone in the milk of the animal, the onset of oestrus being indicated by a concentration of progesterone below a predetermined concentration.
Claim 9
Dependent Claim
57 words
A method for detecting pregnancy in a domestic milk-producing animal comprising using an enzyme immunoassay according to claim 5 to monitor the concentration of progesterone in the milk of the animal, pregnancy being indicated by the lack of a drop in the concentration of progesterone below a predetermined concentration at a time when oestrus would be expected.
Claim 10
Dependent Claim
45 words
A method for detecting pregnancy in a milk-producing domestic animal comprising using an enzyme immunoassay according to claim 6 to monitor the concentration of oestrone sulphate in the milk of an animal, pregnancy being indicated by a concentration of oestrone sulphate above a predetermined concentration.
Claim 11
Dependent Claim
14 words
A method according to claim 7 wherein the domestic milk-producing animal is a cow.
Claim 12
Dependent Claim
13 words
A method according to claim 8 wherein the domestic milk-producing animal is cow.
Claim 13
Dependent Claim
13 words
A method according to claim 9 wherein the domestic milk-producing animal is cow.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-05-05 | 1989-11-04 | 1990-05-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-05-05 | 1993-11-04 | 1994-05-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-05-05 | 1997-11-04 | 1998-05-06 | $7700.00 | Window Closed / Lapsed |