US4783336A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Polyacrolein-type microspheres
Assignee: Yeda Research and Development Co Ltd
•
Filing: 1985-09-04
•
Granted: 1988-11-08
•
Expires: 2005-09-04
Abstract
Abstract Suspension polymerization of acrolein type compounds in the presence of appropriate surfactants results in the formation of microspheres in size ranging from 0.03 to 80 microns. Fluorescent and magnetic microspheres are obtained by carrying out the same polymerization in the presence of appropriate fluorochromic or ferrofluidic compounds, respectively. Hybrido-polyacrolein microspheres are obtained by grafting one type of such microspheres on another type. Immunomicrospheres were formed by binding covalently at physiological pH appropriate proteins to the microspheres. The immunomicrospheres can be used for various biological applications, such as specific markers for labeling cell surface receptors, for cell separation, for diagnostic purposes, etc.
Inventors
- 👤 Shlomo Margel
- 👤 Uzi Beitler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 10:00
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/48; A61K9/50; A61K9/51; A61K9/5107; A61K9/513; A61K9/5138; A61K39/00; A61K39/395; A61K39/44; A61K47/00; A61K47/50; A61K47/51; A61K47/56; A61K47/58; A61K9/5094; C; C08; C08F; C08F16/00; C08F16/34; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54326; G01N33/5434; G01N2446/00; G01N2446/20; G01N2446/30; G01N2446/40; G01N2446/80; G01N2446/86; Y; Y10; Y10S; Y10S526/00; Y10S526/909; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2989; Y10T428/2991; Y10T428/2998; Y10T436/00; Y10T436/25; Y10T436/25375
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
98 words
A polyacrolein-type microsphere produced by subjecting an aqueous mixture of a polymerizable acrolein-type monomer to polymerization in the presence of an effective amount of a suitable ionic surfactant which promotes uniform microsphere formation upon polymerization of the monomer so as to form a polyacrolein-type microsphere, the polymerization being effected at a pH between 8.0 and 13.7, and recovering the resulting microsphere and purifying the microsphere so recovered, the microsphere having an aldehyde content of about 2.9 mmol/gram of microspheres, a double-bond content of about 10.9 mmol/gram of microspheres and a carboxyl content of about 0.34 mmol/gram of microspheres.
Claim 2
Dependent Claim
98 words
A polyacrolein-type microsphere produced by subjecting an aqueous mixture of a polymerizable acrolein-type monomer to polymerization in the presence of an effective amount of a suitable ionic surfactant which promotes uniform microsphere formation upon polymerization of the monomer so as to form a polyacrolein-type microsphere, the polymerization being effected at a pH between 8.0 and 13.7, and recovering the resulting microsphere and purifying the microsphere so recovered, the microsphere having an aldehyde content of about 2.9 mmol/gram of microspheres, a double-bond content of about 10.9 mmol/gram of microspheres and a carboxyl content of about 0.34 mmol/gram of microspheres.
Claim 3
Dependent Claim
13 words
A polyacrolein-type microsphere of claim 1 having a diameter from0.03 to 80 microns.
Claim 4
Dependent Claim
14 words
A polyacrolein microsphere of claim 1, wherein the ionic surfactant is PGL-NaHSO 3 .
Claim 5
Dependent Claim
17 words
A polyacrolein-type microsphere of claim 1, wherein the mixture and resulting microsphere additionally contain a fluorescent dye.
Claim 6
Dependent Claim
15 words
A polyacrolein-type microsphere of claim 1, wherein the mixture and resulting microsphere contain ferrofluidic material.
Claim 7
Dependent Claim
19 words
A polyacrolein-type microsphere of claim 1 to which a compound containing at least one primary amine group is bound.
Claim 8
Dependent Claim
18 words
A polyacrolein-type microsphere of claim 6, wherein the compound is a drug, antibody, antigen, enzyme or other protein.
Claim 9
Dependent Claim
119 words
A polyacrolein-type microsphere having a diameter from 0.03 to 80 microns produced by subjecting under an inert atmosphere and suitable conditions an aqueous mixture of a polymerizable acrolein-type monomer to polymerization in the presence of an effective amount of a suitable surfactant which promotes uniform microsphere formation upon polymerization of the monomer, an oxidizing agent and reducing agent, the oxidizing agent and reducing agent both being present in amounts capable of initiating a redox reaction so as to effect polymerization of the acrolein-type monomer and microsphere formation, recovering the resulting microsphere and purifying the microsphere so recovered, the microsphere having an aldehyde content of about 12.0 mmol/gram of microspheres and a double-bond content of about 1.6 mmol/gram of microspheres.
Claim 10
Dependent Claim
119 words
A polyacrolein-type microsphere having a diameter from 0.03 to 80 microns produced by subjecting under an inert atmosphere and suitable conditions an aqueous mixture of a polymerizable acrolein-type monomer to polymerization in the presence of an effective amount of a suitable surfactant which promotes uniform microsphere formation upon polymerization of the monomer, an oxidizing agent and reducing agent, the oxidizing agent and reducing agent both being present in amounts capable of initiating a redox reaction so as to effect polymerization of the acrolein-type monomer and microsphere formation, recovering the resulting microsphere and purifying the microsphere so recovered, the microsphere having an aldehyde content of about 12.0 mmol/gram of microspheres and a double-bond content of about 1.6 mmol/gram of microspheres.
Claim 11
Dependent Claim
17 words
A polyacrolein microsphere of claim 8, wherein the monomer is acrolein and the surfactant is polyethylene oxide.
Claim 12
Dependent Claim
17 words
A polyacrolein-type microsphere of claim 8, wherein the mixture and the resulting microsphere contain a fluorescent dye.
Claim 13
Dependent Claim
16 words
A polyacrolein-type microsphere of claim 8, wherein the mixture and the resulting microsphere contain ferrofluidic material.
Claim 14
Dependent Claim
19 words
A polyacrolein-type microsphere of claim 8 to which a compound containing at least one primary amine group is bound.
Claim 15
Dependent Claim
18 words
A polyacrolein-type microsphere of claim 12, wherein the compound is a drug, antibody, antigen, enzyme or other protein.
References Cited (Backward Citations)
US3824114A
Cited
US4003846A
Cited
US4046720A
Cited
US4269821A
Cited
US4157323A
Cited
US4267235A
Cited
US4413070A
Cited
US4552812A
Cited
US8183217B2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-11-08 | 1992-05-09 | 1992-11-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-11-08 | 1996-05-09 | 1996-11-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-11-08 | 2000-05-09 | 2000-11-08 | $7700.00 | Window Closed / Lapsed |