US4267235A
Expired / Lapsed
US
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Polyglutaraldehyde microspheres
Assignee: California Institute of Technology
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Filing: 1979-03-19
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Granted: 1981-05-12
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Expires: 1999-03-19
Abstract
Abstract Suspension polymerization of aqueous glutaraldehyde in basic conditions in presence of surfactants such as isobutoxy acrylamide copolymers and/or cross-linking agents yield cross-linked, water insoluble glutaraldehyde polymer microspheres ranging in size from 200 A to 10 mu . Addition of fluorochromes, e.g., FITC, during polymerization yields microspheres of high fluorescent intensity and addition of a suspension of metal containing particles such as Fe3O4 during polymerization yields magnetic microspheres. Immunopolyglutaraldehyde microspheres can be obtained by interacting the polyglutaraldehyde microspheres with antibodies. The immunomicrospheres were used to label and separate cell subpopulations. The labeling specificity is considerably improved by increasing the amount of isobutoxy polyacrylamide incorporated in the microspheres.
Inventors
- 👤 Alan Rembaum
- 👤 Shlomo Margel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 18:58
CPC Classifications
B; B01; B01J; B01J13/00; B01J13/02; B01J13/06; B01J13/14; B01J13/18; B01J13/185; A; A61; A61K; A61K39/00; A61K39/395; A61K39/44; B03; B03C; B03C1/00; B03C1/005; B03C1/01; C; C08; C08G; C08G12/00; C08G6/00; C08G8/00; C08L; C08L61/00; C08L61/02; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/585; Y; Y10; Y10S; Y10S530/00; Y10S530/808; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/816; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2991; Y10T428/2993; Y10T428/2996; Y10T428/2998
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
87 words
Microspheres having a diameter from 200 A to 10μ consisting essentially of water-insoluable copolymer of glutaraldehyde having a repeating conjugated aldehyde containing unit of the formula: ##STR2## cross linked with 0.1 to 10% by weight of a water-dispersible or water-soluble organic cross-linking agent containing at least two functional groups reactive with said aldehyde group selected from amine, hydroxyl, imine mercaptan or amide and said microspheres containing 0.5 to 40% by weight of metal containing particles having an atomic number above 50 and a diameter below 1000 A.
Claim 2
Dependent Claim
87 words
Microspheres having a diameter from 200 A to 10μ consisting essentially of water-insoluable copolymer of glutaraldehyde having a repeating conjugated aldehyde containing unit of the formula: ##STR2## cross linked with 0.1 to 10% by weight of a water-dispersible or water-soluble organic cross-linking agent containing at least two functional groups reactive with said aldehyde group selected from amine, hydroxyl, imine mercaptan or amide and said microspheres containing 0.5 to 40% by weight of metal containing particles having an atomic number above 50 and a diameter below 1000 A.
Claim 3
Dependent Claim
14 words
Microspheres according to claim 1 in which the cross-linking agent is also a surfactant.
Claim 4
Dependent Claim
14 words
Microspheres according to claim 2 in which the cross-linking agent is an acrylamide polymer.
Claim 5
Dependent Claim
29 words
Microspheres according to claim 3 in which the cross-linking agent is a copolymer of 10 to 70% acrylamide and 10 to 70% of an alkyl-acrylamide or an alkyloxyalkyl acrylamide.
Claim 6
Dependent Claim
16 words
Microspheres according to claim 1 in which the cross-linking agent contains 2 to 20 carbon atoms.
Claim 7
Dependent Claim
13 words
Microspheres according to claim 5 in which the cross-linking agent is a diamine.
Claim 8
Dependent Claim
12 words
Microspheres according to claim 1 in which the particles are magnetically attractable.
Claim 9
Dependent Claim
15 words
Microspheres according to claim 7 in which the particles comprise Fe 3 O 4 .
Claim 10
Dependent Claim
8 words
Microspheres according to claim 1 containing fluorogen groups.
Claim 11
Dependent Claim
29 words
Microspheres according to claim 9 in which the fluorogen groups are the reaction products for acrolein groups and a compound that forms a fluorogen on reaction with said groups.
Claim 12
Dependent Claim
21 words
Microspheres according to claim 9 in which the fluorogen is the reaction product of aldehyde groups with an aldehyde reactive fluorochrome.
Claim 13
Dependent Claim
20 words
Microspheres according to claim 11 in which the fluorochrome is selected from aminofluorescein, 9-amino acridine, propidium bromide, or fluorescein isothiocyanate.
Claim 14
Dependent Claim
12 words
Microspheres according to claim 1 bound to protein through said aldehyde groups.
Claim 15
Dependent Claim
18 words
Microspheres according to claim 13 in which the protein is selected from antibodies, antigens, immunoglobulins, lymphocytes or hemoglobin.
Claim 16
Dependent Claim
12 words
Microspheres according to claim 14 in which the protein is an antibody.
References Cited (Backward Citations)
US3957741A
Cited
US4070246A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1984-05-11 | 1984-11-10 | 1985-05-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-05-11 | 1988-11-10 | 1989-05-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-05-11 | 1992-11-10 | 1993-05-12 | $7700.00 | Window Closed / Lapsed |