US4777139A
Expired / Lapsed
US
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Hematology control or calibrator with red cell components of enhanced stability
Assignee: IL Holding Spa
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Filing: 1987-06-25
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Granted: 1988-10-11
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Expires: 2007-06-25
Abstract
Abstract Red cells are exposed to an unsaturated aldehyde such as acrolein (propenal) under conditions sufficient to increase the stability of the cells without impairing the ability of a lysing reagent to lyse the cells. After treatment, the treated cells are washed and are suspended in a stabilizing suspension medium.
Inventors
- 👤 Show-Chu Wong
- 👤 Harbans S. Deol
- 👤 Debra L. Harz
- 👤 Jill H. Davidson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 04:19
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/96; G01N2496/00; G01N2496/05; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666; Y10T436/107497; Y10T436/108331
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
63 words
A hematology control or calibrator comprising a suspension of treated erythrocytes in a stabilizing medium, characterized by the treated erythrocytes having been exposed to a aliphatic unsaturated aldehyde of 3-6 carbons under conditions sufficient to increase the stability of the treated erythrocytes in the stabilizing medium without impairing the ability of a lysing reagent to lyse the treated erythrocytes in a hematology analyzer.
Claim 2
Dependent Claim
63 words
A hematology control or calibrator comprising a suspension of treated erythrocytes in a stabilizing medium, characterized by the treated erythrocytes having been exposed to a aliphatic unsaturated aldehyde of 3-6 carbons under conditions sufficient to increase the stability of the treated erythrocytes in the stabilizing medium without impairing the ability of a lysing reagent to lyse the treated erythrocytes in a hematology analyzer.
Claim 3
Dependent Claim
15 words
The hematology control or calibrator of claim 1 wherein the aliphatic unsaturated aldehyde is propenal.
Claim 4
Dependent Claim
48 words
The hematology control or calibrator of claim 2 wherein the treated erythrocytes have been exposed to propenal at a concentration of about 0.01% to about 1.0% for a time of about 5 minutes to about 24 hours at a temperature from about 0° C. to about 30° C.
Claim 5
Dependent Claim
29 words
The hematology control or calibrator of claim 3 wherein the temperature is about 20°-25° C., the time is about 1 to 4 hours and the concentration is about 0.02-0.2%.
Claim 6
Dependent Claim
27 words
The hematology control or calibrator of claim 3 wherein the temperature is about 0°-5° C., the time is about 15-30 hours and the concentration is about 0.02-0.2%.
Claim 7
Dependent Claim
90 words
A process for preparing a hematology control or calibrator comprising the steps: (a) exposing washed erythrocytes to an unsaturated aldehyde at a treatment concentration and temperature for a treatment time period, (b) after the treatment time period, washing the treated erythrocytes, and (c) suspending the treated and washed erythrocytes in a stabilizing suspending medium the treatment concentration, temperature and time being sufficient to increase the stability of the treated erythrocytes in suspending medium without impairing the ability of a lysing reagent to lyse the treated erythrocytes in a hematology analyzer.
Claim 8
Dependent Claim
90 words
A process for preparing a hematology control or calibrator comprising the steps: (a) exposing washed erythrocytes to an unsaturated aldehyde at a treatment concentration and temperature for a treatment time period, (b) after the treatment time period, washing the treated erythrocytes, and (c) suspending the treated and washed erythrocytes in a stabilizing suspending medium the treatment concentration, temperature and time being sufficient to increase the stability of the treated erythrocytes in suspending medium without impairing the ability of a lysing reagent to lyse the treated erythrocytes in a hematology analyzer.
Claim 9
Dependent Claim
12 words
The process of claim 6 wherein the aliphatic unsaturated aldehyde is propenal.
Claim 10
Dependent Claim
38 words
The process of claim 7 wherein the treatment concentration is about 0.01% to about 0.5%, the treatment temperature is about 0° C. to about 30° C. and the treatment time is about 5 minutes to about 24 hours.
Claim 11
Dependent Claim
36 words
The process of claim 8 wherein the treatment concentration is about 0.02 to about 0.2%, the treatment temperature is about 20° C. to about 25° C. and the treatment time is about 1 to 4 hours.
Claim 12
Dependent Claim
34 words
The process of claim 8 wherein the treatment concentration is about 0.02% to about 0.2%, the treatment temperature is about 0° C. to about 5° C. and the treatment time is about 15-30 hours.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-10-11 | 1992-04-11 | 1992-10-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-10-11 | 1996-04-11 | 1996-10-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-10-11 | 2000-04-11 | 2000-10-11 | $7700.00 | Window Closed / Lapsed |